Results 91 to 100 of about 1,295,509 (307)
Zhongguo shiyan zhenduanxue, 2014 目的本试验检测自发性高血压大鼠（SHR）血液中miR-448-5p、miR-128、miR-146a和miR-24-2*四种小分子RNA（miRNA）的表达,探讨高血压疾病与miRNA表达水平相关性,为人类高血压诊断及治疗提供新的靶标。方法应用荧光定量PCR方法对SHR大鼠和正常大鼠血液中miR-448-5p、miR-128、miR-146a和miR-24-2*表达量进行检测。结果与正常对照组相比,SHR大鼠血液中miR-448-5p、miR-128、miR-146a和miR-24-2 ...
鞠传静 +4 more
doaj
Biotechnology & Biotechnological Equipment, 2017 Extraction of RNA of high quality and integrity is essential for gene expression studies and all downstream RNA-based techniques. The leaves of 16 merit Malaysian rice varieties were used to isolate total RNA using five different methods.
Parisa Azizi +7 more
doaj +1 more source
Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos
BMC Developmental Biology, 2005 BackgroundReal-time quantitative PCR is a sensitive and very efficient technique to examine gene transcription patterns in preimplantation embryos, in order to gain information about embryo development and to optimize assisted reproductive technologies ...
K. Goossens +5 more
semanticscholar +1 more source
Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA
FEBS Open Bio, EarlyView.Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley +1 more source
Selection and Validation of Reference Genes for Quantitative Real-time PCR in Gentiana macrophylla
Frontiers in Plant Science, 2016 Real time quantitative PCR (RT-qPCR or qPCR) has been extensively applied for analyzing gene expression because of its accuracy, sensitivity, and high throughput.
Yi-Han He +4 more
semanticscholar +1 more source
Experimental & molecular medicine, 2002 So far, quantitative techniques, such as PCR and FISH, have been used to detect of DNA and RNA. However, it is difficult to measure and compare the exact amount of amplified products with the results of endpoint analysis in conventional PCR techniques.
openaire +1 more source
Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound
FEBS Open Bio, EarlyView.We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda +4 more
wiley +1 more source
Frontiers in Veterinary ScienceIntroductionThe African swine fever (ASF) is a viral disease of significant socio-economic and animal health impact, commonly diagnosed using molecular techniques.
Silvia Dei Giudici +7 more
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Letters in Applied Microbiology, 2007 Aims: The aim of the study was the development of a sensitive human‐specific quantitative real‐time PCR assay for microbial faecal source tracking (MST) in alpine spring water.
G. Reischer +4 more
semanticscholar +1 more source