Results 21 to 30 of about 1,295,509 (307)

A quantitative real-time PCR method for absolute telomere length

BioTechniques, 2008
Telomere shortening is an important risk factor for cancer and accelerated aging. Here we describe the development of a simple and reproducible method to measure absolute telomere length. Based on Cawthon's quantitative real-time PCR (qRT-PCR) assay, our
Nathan J. O'Callaghan, Varinderpal S. Dhillon, Philip Thomas, Michael Fenech   +3 more
doaj   +1 more source

Low-Cost Battery-Powered and User-Friendly Real-Time Quantitative PCR System for the Detection of Multigene

Micromachines, 2020
Real-time polymerase chain reaction (PCR) is the standard for nucleic acid detection and plays an important role in many fields. A new chip design is proposed in this study to avoid the use of expensive instruments for hydrophobic treatment of the ...
Junru An, Yangyang Jiang, Bing Shi, Di Wu, Wenming Wu   +4 more
doaj   +1 more source

Exogenous Reference RNA for Normalization of Real-Time Quantitative PCR

BioTechniques, 2003
We have utilized an in vitro transcribed 3′ mRNA fragment of the plant gene ribulose bisphosphate carboxylase (RuBisCO) as an exogenous standard for normalization of quantitative PCR data. Both K562 cells and primary erythroid CD34+ progenitor cells were
Reginald D. Smith, Benjamin Brown, Pranvera Ikonomi, Alan N. Schechter   +3 more
doaj   +1 more source

MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments

BMC Molecular Biology, 2010
The conclusions of thousands of peer-reviewed publications rely on data obtained using fluorescence-based quantitative real-time PCR technology. However, the inadequate reporting of experimental detail, combined with the frequent use of flawed protocols ...
S. Bustin, J. Beaulieu, J. Huggett, R. Jaggi, F. Kibenge, P. Olsvik, L. Penning, S. Toegel   +7 more
semanticscholar   +1 more source

DETERMINAZIONI VIRALI QUANTITATIVE MEDIANTE “PCR REAL TIME” CON IL SISTEMA “ROTOR GENETM”

Microbiologia Medica, 2005
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M.G. Marin, P. Turconi, F. Poma, F. Calamani, P. Guarneri, L. Berardi, L. Lattuada   +6 more
doaj   +1 more source

Does the human placenta delivered at term have a microbiota? Results of cultivation, quantitative real-time PCR, 16S rRNA gene sequencing, and metagenomics

American Journal of Obstetrics and Gynecology, 2019
Background The human placenta has been traditionally viewed as sterile, and microbial invasion of this organ has been associated with adverse pregnancy outcomes.
K. Theis, R. Romero, Andrew D. Winters, Jonathan M. Greenberg, N. Gomez-Lopez, Ali Alhousseini, J. Bieda, E. Maymon, P. Pacora, J. Fettweis, G. Buck, K. Jefferson, J. Strauss, O. Erez, S. Hassan   +14 more
semanticscholar   +1 more source

An overview of technical considerations when using quantitative real-time PCR analysis of gene expression in human exercise research

PLoS ONE, 2018
Gene expression analysis by quantitative PCR in skeletal muscle is routine in exercise studies. The reproducibility and reliability of the data fundamentally depend on how the experiments are performed and interpreted. Despite the popularity of the assay,
J. Kuang, Xu Yan, A. Genders, C. Granata, D. Bishop   +4 more
semanticscholar   +1 more source

FungiQuant: A broad-coverage fungal quantitative real-time PCR assay

BMC Microbiology, 2012
Background Fungal load quantification is a critical component of fungal community analyses. Limitation of current approaches for quantifying the fungal component in the human microbiome suggests the need for new broad-coverage techniques.
Liu Cindy M, Kachur Sergey, Dwan Michael G, Abraham Alison G, Aziz Maliha, Hsueh Po-Ren, Huang Yu-Tsung, Busch Joseph D, Lamit Louis J, Gehring Catherine A, Keim Paul, Price Lance B   +11 more
doaj   +1 more source

Development of a Real-Time Quantitative PCR Based on a TaqMan-MGB Probe for the Rapid Detection of Theileria haneyi

Microorganisms, 2023
Equine piroplasmosis (EP) is a parasitic disease caused by Theileria equi (T. equi), Babesia caballi (B. caballi) and Theileria haneyi (T. haneyi). This disease is considered to be reportable by the World Organization for Animal Health (WOAH).
Bingqian Zhou, Guangpu Yang, Zhe Hu, Kewei Chen, Wei Guo, Xiaojun Wang, Cheng Du   +6 more
doaj   +1 more source

Validation and Evaluation of Reference Genes for Quantitative Real-Time PCR in Macrobrachium Nipponense

International Journal of Molecular Sciences, 2018
Quantitative real-time PCR (qPCR) is widely used in molecular biology, although the accuracy of the quantitative results is determined by the stability of the reference genes used.
Yu-Ning Hu, H. Fu, H. Qiao, Sheng-Ming Sun, Wen-Yi Zhang, Shu-Bo Jin, Su-Fei Jiang, Yong-Sheng Gong, Yi-Wei Xiong, Yan Wu   +9 more
semanticscholar   +1 more source