Results 31 to 40 of about 21,194,347 (300)

Exogenous Reference RNA for Normalization of Real-Time Quantitative PCR

BioTechniques, 2003
We have utilized an in vitro transcribed 3′ mRNA fragment of the plant gene ribulose bisphosphate carboxylase (RuBisCO) as an exogenous standard for normalization of quantitative PCR data. Both K562 cells and primary erythroid CD34+ progenitor cells were
Reginald D. Smith, Benjamin Brown, Pranvera Ikonomi, Alan N. Schechter   +3 more
doaj   +1 more source

Detection of Tumor Cell-Specific mRNA in the Peripheral Blood of Patients with Breast Cancer-Evaluation of Several Markers with Real-Time Reverse Transcription-PCR [PDF]

, 2013
It is widely known that cells from epithelial tumors, e. g., breast cancer, detach from their primary tissue and enter blood circulation. We show that the presence of circulating tumor cells (CTCs) in samples of patients with primary and metastatic ...
Simone Hofmann, Kölbl, Alexandra C., Jeschke, Udo, Klaus Friese, Andergassen, Ulrich, Neugebauer, Julia, Alexandra Kölbl, Udo Jeschke, Matthias Ilmer, Julia Neugebauer, Ilmer, Matthias, Ulrich Andergassen, Engelstädter, Verena, Schindlbeck, Christian, Hofmann, Simone, Hutter, Stefan, Stefan Hutter, Ra C. Kölbl, Friese, Klaus, Verena Engelstädter, Christian Schindlbeck   +20 more
core   +1 more source

FungiQuant: A broad-coverage fungal quantitative real-time PCR assay

BMC Microbiology, 2012
Background Fungal load quantification is a critical component of fungal community analyses. Limitation of current approaches for quantifying the fungal component in the human microbiome suggests the need for new broad-coverage techniques.
Liu Cindy M, Kachur Sergey, Dwan Michael G, Abraham Alison G, Aziz Maliha, Hsueh Po-Ren, Huang Yu-Tsung, Busch Joseph D, Lamit Louis J, Gehring Catherine A, Keim Paul, Price Lance B   +11 more
doaj   +1 more source

Development of a Real-Time Quantitative PCR Based on a TaqMan-MGB Probe for the Rapid Detection of Theileria haneyi

Microorganisms, 2023
Equine piroplasmosis (EP) is a parasitic disease caused by Theileria equi (T. equi), Babesia caballi (B. caballi) and Theileria haneyi (T. haneyi). This disease is considered to be reportable by the World Organization for Animal Health (WOAH).
Bingqian Zhou, Guangpu Yang, Zhe Hu, Kewei Chen, Wei Guo, Xiaojun Wang, Cheng Du   +6 more
doaj   +1 more source

Numerical and experimental study of a droplet-based PCR chip [PDF]

, 2007
A two-temperature continuous-flow polymerase chain reaction (PCR) polymer chip has been constructed that takes advantage of droplet technology to avoid sample contamination and adsorption at the surface.
MacAskill, Alexandra, Goddard, N.J., Emerson, David, Macaskill A, Emerson DR, Day, P. J R, Fielden, P. R., Emerson, D. R., Zhang, Y. H., Mohr S, Day, Philip; id_orcid, Day PJR, Barber RW, Fielden, P.R., Barber, R. W., Zhang YH, Zhang, Yonghao, Goddard NJ, Goddard, N. J., Barber, Robert W., Macaskill, A., Mohr, S., Fielden PR, Day, P.J.R.   +23 more
core   +4 more sources

DETERMINAZIONI VIRALI QUANTITATIVE MEDIANTE “PCR REAL TIME” CON IL SISTEMA “ROTOR GENETM”

Microbiologia Medica, 2005
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M.G. Marin, P. Turconi, F. Poma, F. Calamani, P. Guarneri, L. Berardi, L. Lattuada   +6 more
doaj   +1 more source

The performance of semi-quantitative differential PCR is similar to that of real-time PCR for the detection of the MYCN gene in neuroblastomas

Brazilian Journal of Medical and Biological Research, 2009
Amplification of the MYCN gene in neuroblastomas is a potent biological marker of highly aggressive tumors, which are invariably fatal unless sound clinical management is applied. To determine the usefulness of semi-quantitative differential PCR (SQ-PCR)
A.C.M.F. Souza, D.R.V. Souza, S.S. Sanabani, R.R. Giorgi, I. Bendit   +4 more
doaj   +1 more source

Detection and quantification of Verticillium dahliae and V. longisporum by droplet digital PCR versus quantitative real-time PCR

Frontiers in Cellular and Infection Microbiology, 2022
Vascular wilt, caused by Verticillium dahliae and V. longisporum, limits the quality and yield of agricultural crops. Although quantitative real-time PCR (qPCR) has greatly improved the diagnosis of these two pathogens over traditional, time-consuming ...
Di Wang, Xinya Jiao, Haijiang Jia, Shumei Cheng, Xi Jin, Youhua Wang, Yunhua Gao, Xiaofeng Su   +7 more
doaj   +1 more source

Real-time quantitative RT-PCR primers.

, 2021
Real-time quantitative RT-PCR primers.
Gabriel O. Akerele (11714547), Revathi Shanmugasundaram (176342), Sankar Renu (6983570), Ramesh K. Selvaraj (10305301), Renukaradhy J. Gourapura (11714550), Keila Y. Acevedo-Villanueva (8763321)   +5 more
core   +1 more source

Real-time quantitative PCR primers.

, 2020
Real-time quantitative PCR primers.
Samantha A. Brooks (8693760), Nathan B. Sutter (7388264), Sara E. Kalla (3310455), Heather Marie Holl (8693757), Mohammed Ali Al Abri (8693754)   +4 more
core   +1 more source