Results 31 to 40 of about 22,071,005 (304)

Quantitative real-time PCR primers.

, 2013
Quantitative real-time PCR primers.
Qi Jin (12159), Jianmin Wang (59025), Zhiqiang Wu (102384)   +2 more
core   +2 more sources

Quantitative real-time PCR primers.

, 2014
Quantitative real-time PCR primers.
Elena G. Ruano (461847), Ramon Gomis (35888), Felicia A. Hanzu (575738), Dulce Momblan (653824), Francesc Carmona (653825), Ana Lucia C. Figueroa (437723), Gloria Aranda (653823), Elaine Vieira (174928), Josep Vidal (631949)   +8 more
core   +2 more sources

Quantitative Real-Time PCR

, 2006
Changes in mRNA expression levels occur during physiological and pathological processes in the cardiovascular system. An increase inDNAtranscription results in increasedmRNAlevels and will subsequently result in increased protein levels that regulate processes inside and outside the cell.
Sluijter, J. P. G., Pasterkamp, G., de Kleijn, D. P. V.   +2 more
openaire   +1 more source

Physical Simulation-Based Calibration for Quantitative Real-Time PCR

Applied Sciences
The fluorescence quantitative polymerase chain reaction (qPCR) instrument has been widely used in molecular biology applications, where the reliability of the qPCR performance directly affects the accuracy of its detection results.
Tianyu Zhu, Xin Liu, Xinqing Xiao
doaj   +1 more source

Development of a droplet digital PCR method for detection of porcine circovirus 4

BMC Veterinary Research, 2023
Background Porcine circovirus 4 (PCV4), a newly emerging virus that was first discovered in 2019, may pose a potential threat to the pig industry. Droplet digital PCR (ddPCR) is an absolute quantitative method that has high sensitivity and accuracy.
Yangkun Liu, Xinru Zhang, Xueying Han, Jiaxing Liu, Lunguang Yao   +4 more
doaj   +1 more source

Evaluation and validation of reference genes in Cymbidium faberi for real-time quantitative PCR

BioTechniques, 2022
For all organs at all Cymbidium faberi stages, ACT, UBQ3 and GAPDH can be selected as reference genes. For organs of the vegetative stage, UBQ2 and UBQ3 can be chosen for analysis of normalized gene expression. For the bud stage, ACT and UBQ3 can be used
Yunfang Tian, Zhigang Chu, Linqing Wang, Huiyu Wang, Xiuyun Yuan, Si Wu, Yuzhen Yang   +6 more
doaj   +1 more source

A novel real-time PCR for Listeria monocytogenes that monitors analytical performance via an internal amplification control [PDF]

, 2005
We describe a novel quantitative real-time (Q)-PCR assay for Listeria monocytogenes based on the coamplification of a target hly gene fragment and an internal amplification control (IAC). The IAC is a chimeric double-stranded DNA containing a fragment of
Vázquez-Boland, JA, Pla, Maria, Monzó, HJ, Rodríguez-Lázaro, AD, Scortti, MM, Rodríguez-Lázaro, David, Monzó, Hector J, Pla, M, Scortti, Mariela, Vazquez-Boland, Jose A   +9 more
core  

Construction of an adult barnacle (Balanus amphitrite) cDNA library and selection of reference genes for quantitative RT-PCR studies [PDF]

, 2009
De Gregoris TB, Borra M, Biffali E, et al. Construction of an adult barnacle (Balanus amphitrite) cDNA library and selection of reference genes for quantitative RT-PCR studies. BMC Molecular Biology.
De Gregoris, Tristano Bacchetti, Clare, Anthony S., Biffali, Elio, Elio Biffali, Thomas Bekel, Bekel, Thomas, Richard R Kirby, Borra, Marco, J Grant Burgess, Marco Borra, Burgess, J. Grant, Bacchetti De Gregoris, Tristano, Anthony S Clare, Kirby, Richard R., Tristano Bacchetti De Gregoris   +14 more
core   +1 more source

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source