Results 11 to 20 of about 22,071,005 (304)

Detection and quantification of Verticillium dahliae and V. longisporum by droplet digital PCR versus quantitative real-time PCR

Frontiers in Cellular and Infection Microbiology, 2022
Vascular wilt, caused by Verticillium dahliae and V. longisporum, limits the quality and yield of agricultural crops. Although quantitative real-time PCR (qPCR) has greatly improved the diagnosis of these two pathogens over traditional, time-consuming ...
Di Wang, Xinya Jiao, Haijiang Jia, Shumei Cheng, Xi Jin, Youhua Wang, Yunhua Gao, Xiaofeng Su   +7 more
doaj   +3 more sources

Real-Time Quantitative PCR: Primer Design, Reference Gene Selection, Calculations and Statistics. [PDF]

Metabolites, 2023
Chen Z, Halford NG, Liu C.
europepmc   +2 more sources

Real-time quantitative PCR primers.

, 2021
Real-time quantitative PCR primers.
Jolene Carlson (4044971), Jens Peter Teifke (10136422), Gregor Meyers (528376), Christiane Pfarrer (3135114), Julia Sehl-Ewert (11812757), Robert Kammerer (72621)   +5 more
core   +1 more source

Real-time quantitative RT-PCR primers.

, 2021
Real-time quantitative RT-PCR primers.
Gabriel O. Akerele (11714547), Revathi Shanmugasundaram (176342), Sankar Renu (6983570), Ramesh K. Selvaraj (10305301), Renukaradhy J. Gourapura (11714550), Keila Y. Acevedo-Villanueva (8763321)   +5 more
core   +1 more source

A Parylene Real Time PCR Microdevice [PDF]

, 2010
The polymerase chain reaction (PCR) is a powerful biochemical assay that is used in virtually all biochemical labs. By specifically amplifying a small sample of DNA, this technique is useful in the fields of paternity testing, forensics, and virus ...
Quach, Quoc Chan (Brandon)
core   +1 more source

A real-time PCR assay to estimate Leishmania chagasi load in its natural sand fly vector Lutzomyia longipalpis. [PDF]

, 2008
Leishmania chagasi, transmitted mainly by Lutzomyia longipalpis sand flies, causes visceral leishmaniasis and atypical cutaneous leishmaniasis in Latin America.
Ranasinghe, Shalindra, Ranasinghe, S., Rogers, Matthew E., Hamilton, J. G. C., Maingon, Rhayza DC, Rogers, Matthew E, Bates, Paul, Hamilton, James GC, Maingon, R. D. C., Bates, Paul A   +9 more
core   +1 more source

Comparison of different real-time PCR chemistries and their suitability for detection and quantification of genetically modified organisms [PDF]

, 2008
Background The real-time polymerase chain reaction is currently the method of choice for quantifying nucleic acids in different DNA based quantification applications. It is widely used also for detecting and quantifying genetically modified components in
Cankar, K., Buh Gašparič, Meti, Gasparic, M.B., Gruden, Kristina, Cankar, Katarina, Gruden, K., Zel, J., Žel, Jana, Kristina Gruden, Katarina Cankar, Meti Buh Gašparič, Jana Žel   +11 more
core   +1 more source

Presence of SARS-CoV-2 in human tears detected by quantitative real time PCR (qRT-PCR)

Medical Journal of Dr. D.Y. Patil Vidyapeeth, 2022
Background: COVID-19 has caused a pandemic since the end of the year 2019. Controversy regarding the presence of the SARS-CoV-2 in tears and conjunctival sac has created suspense throughout.
Neeta Mishra, Chandrashekhar Raut, Neelam Prasad, J S Bhawalkar, Safia Farooqui, Shahzad Mirza, Veshal V Madan   +6 more
doaj   +1 more source

Quantitative real-time RT-PCR – a perspective [PDF]

Journal of Molecular Endocrinology, 2005
Abstract The real-time reverse transcription polymerase chain reaction (RT-PCR) uses fluorescent reporter molecules to monitor the production of amplification products during each cycle of the PCR reaction. This combines the nucleic acid amplification and detection steps into one homogeneous assay and obviates the need for gel ...
S A, Bustin, V, Benes, T, Nolan, M W, Pfaffl   +3 more
openaire   +2 more sources

A quantitative real-time PCR method for absolute telomere length

BioTechniques, 2008
Telomere shortening is an important risk factor for cancer and accelerated aging. Here we describe the development of a simple and reproducible method to measure absolute telomere length. Based on Cawthon's quantitative real-time PCR (qRT-PCR) assay, our
Nathan J. O'Callaghan, Varinderpal S. Dhillon, Philip Thomas, Michael Fenech   +3 more
doaj   +1 more source