The International Journal of Biological Markers, 2006 The assessment of ERα, PgR and HER2 status is routinely performed today to determine the endocrine responsiveness of breast cancer samples. Such determination is usually accomplished by means of immunohistochemistry and in case of HER2 amplification by ...
M. Labuhn +8 more
doaj +2 more sources
Real-Time Quantitative PCR: Primer Design, Reference Gene Selection, Calculations and Statistics. [PDF]
Metabolites, 2023 Real-time quantitative PCR is a technique that can measure the content of the target nucleic acid sequence of interest in a given sample. It is mainly divided into absolute and relative quantitative methods.
Chen Z, Halford NG, Liu C.
europepmc +2 more sources
A Parylene Real Time PCR Microdevice [PDF]
, 2010 The polymerase chain reaction (PCR) is a powerful biochemical assay that is used in virtually all biochemical labs. By specifically amplifying a small sample of DNA, this technique is useful in the fields of paternity testing, forensics, and virus ...
Quach, Quoc Chan (Brandon)
core +1 more source
A real-time PCR assay to estimate Leishmania chagasi load in its natural sand fly vector Lutzomyia longipalpis [PDF]
, 2008 Leishmania chagasi, transmitted mainly by Lutzomyia longipalpis sand flies, causes visceral leishmaniasis and atypical cutaneous leishmaniasis in Latin America.
Bates, Paul A. +14 more
core +1 more source
Internally controlled real-time PCR method for quantitative species-specific detection and vapA genotyping of Rhodococcus equi [PDF]
, 2006 We developed a novel quantitative real-time PCR (Q-PCR) method for the soil actinomycete Rhodococcus equi, an important horse pathogen and emerging human pathogen.
Makrai, L +26 more
core +1 more source
Biologia Plantarum, 2012 Germination of Lepidium apetalum Wild. seeds is invariably arrested by cold stress. cDNA-amplified fragment length polymorphism (AFLP) technique was used to isolate genes relevant to chilling stress (4 °C) during seedling emergence. 43 transcript-derived
H. X. Zhao, Q. Li, G. Li, Y. Du
doaj +1 more source
Detection of Salmonella Typhi in Bile by Quantitative Real-Time PCR
Microbiology Spectrum, 2022 In countries where the incidence of typhoid fever is high, fecal material from short-term carriers of Salmonella Typhi contaminates inadequately treated water supplies.
Ellen E. Higginson +9 more
doaj +1 more source
Comparison of different real-time PCR chemistries and their suitability for detection and quantification of genetically modified organisms [PDF]
, 2008 Background The real-time polymerase chain reaction is currently the method of choice for quantifying nucleic acids in different DNA based quantification applications. It is widely used also for detecting and quantifying genetically modified components in
Žel Jana +11 more
core +1 more source
Quantification of Fusarium graminearum and Fusarium culmorum by real-time PCR system and zearalenone assessment in maize [PDF]
, 2012 Zearalenone (ZEA) is a mycotoxin produced by some species of Fusarium, especially by Fusarium graminearum and F. culmorum. ZEA induces hyperoestrogenic responses in mammals and can result in reproductive disorders in farm animals. In the present study, a
Atoui, Ali +3 more
core +1 more source
Detection of Pork-Derived Ingredients Based on Real-time Quantitative PCR
Shipin gongye ke-ji, 2022 In order to accurately and reliably quantify pork-derived ingredients in meat products, single-copy gene (carcinoembryonic antigen-related cell adhesion molecule 2-like, CACA) in pig cell nucleus was screened by bioinformatics methods, with CACA gene as ...
Yao YANG +5 more
doaj +1 more source