Results 21 to 30 of about 21,194,347 (300)

Quantitative real-time RT-PCR – a perspective [PDF]

Journal of Molecular Endocrinology, 2005
Abstract The real-time reverse transcription polymerase chain reaction (RT-PCR) uses fluorescent reporter molecules to monitor the production of amplification products during each cycle of the PCR reaction. This combines the nucleic acid amplification and detection steps into one homogeneous assay and obviates the need for gel ...
S A, Bustin, V, Benes, T, Nolan, M W, Pfaffl   +3 more
openaire   +2 more sources

Evaluating an MRNA based body fluid identification test using sybr green fluorescent dye and real-time PCR [PDF]

, 2011
The requirement to have more definitive and wider ranging body fluid identification (BFI) tests has resulted in a range of mRNA based real-time PCR BFI assays utilising Taqman fluorescent dye.
Connolly, Jo-Ann, Williams, Graham
core   +3 more sources

Preliminary screening of intestinal barrier genes associated with porcine epidemic diarrhea virus infection in pigs

Revista Brasileira de Zootecnia, 2020
To screen intestinal barrier genes associated with porcine epidemic diarrhea virus (PEDV) infection, in the present study we first detected PEDV-infected piglets ( Sus scrofa ) with intestinal damage using quantitative real-time PCR (qPCR) and ...
Xiaoyi Qi, Yafei Xu, Weiyun Qin, Haifei Wang, Shenglong Wu, Wenbin Bao   +5 more
doaj   +1 more source

Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples. [PDF]

, 2008
peer-reviewedBackground: A real-time multiplex PCR assay was developed for the detection of multiple Salmonella serotypes in chicken samples. Poultry-associated serotypes detected in the assay include Enteritidis, Gallinarum, Typhimurium, Kentucky and ...
Sheila McGuinness, McCabe, Evonne, Paul Whyte, Geraldine Duffy, McGuinness, Sheila, Barry, Thomas, Thomas Barry, Edel O'Regan, Séamus Fanning, Duffy, Geraldine, Whyte, Paul, O'Regan, Edel, Evonne McCabe, Catherine Burgess, Burgess, Catherine, Fanning, Seamus   +15 more
core   +1 more source

Low-Cost Battery-Powered and User-Friendly Real-Time Quantitative PCR System for the Detection of Multigene

Micromachines, 2020
Real-time polymerase chain reaction (PCR) is the standard for nucleic acid detection and plays an important role in many fields. A new chip design is proposed in this study to avoid the use of expensive instruments for hydrophobic treatment of the ...
Junru An, Yangyang Jiang, Bing Shi, Di Wu, Wenming Wu   +4 more
doaj   +1 more source

Presence of SARS-CoV-2 in human tears detected by quantitative real time PCR (qRT-PCR)

Medical Journal of Dr. D.Y. Patil Vidyapeeth, 2022
Background: COVID-19 has caused a pandemic since the end of the year 2019. Controversy regarding the presence of the SARS-CoV-2 in tears and conjunctival sac has created suspense throughout.
Neeta Mishra, Chandrashekhar Raut, Neelam Prasad, J S Bhawalkar, Safia Farooqui, Shahzad Mirza, Veshal V Madan   +6 more
doaj   +1 more source

Construction of an adult barnacle (Balanus amphitrite) cDNA library and selection of reference genes for quantitative RT-PCR studies [PDF]

, 2009
De Gregoris TB, Borra M, Biffali E, et al. Construction of an adult barnacle (Balanus amphitrite) cDNA library and selection of reference genes for quantitative RT-PCR studies. BMC Molecular Biology.
De Gregoris, Tristano Bacchetti, Clare, Anthony S., Biffali, Elio, Elio Biffali, Thomas Bekel, Bekel, Thomas, Richard R Kirby, Borra, Marco, J Grant Burgess, Marco Borra, Burgess, J. Grant, Bacchetti De Gregoris, Tristano, Anthony S Clare, Kirby, Richard R., Tristano Bacchetti De Gregoris   +14 more
core   +1 more source

Real-time quantitative PCR primers.

, 2021
Real-time quantitative PCR primers.
Jolene Carlson (4044971), Jens Peter Teifke (10136422), Gregor Meyers (528376), Christiane Pfarrer (3135114), Julia Sehl-Ewert (11812757), Robert Kammerer (72621)   +5 more
core   +1 more source

A quantitative real-time PCR method for absolute telomere length

BioTechniques, 2008
Telomere shortening is an important risk factor for cancer and accelerated aging. Here we describe the development of a simple and reproducible method to measure absolute telomere length. Based on Cawthon's quantitative real-time PCR (qRT-PCR) assay, our
Nathan J. O'Callaghan, Varinderpal S. Dhillon, Philip Thomas, Michael Fenech   +3 more
doaj   +1 more source

Real-time quantitative PCR for analysis of candidate fungal biopesticides against malaria: Technique validation and first applications [PDF]

, 2009
Recent research has indicated that fungal biopesticides could augment existing malaria vector control tools. Here we present a set of methodologies to monitor the in vivo kinetics of entomopathogenic fungi in Anopheles in the presence or absence of ...
Thomas, Matthew B., Bell, Andrew S., Jenkins, Nina, Nina Jenkins, Blanford, Simon, Matthew B. Thomas, Andrew S. Bell, Simon Blanford, Read, Andrew F., Andrew F. Read   +9 more
core   +1 more source