Results 1 to 10 of about 6,818,443 (298)

Improving Protein Expression, Stability, and Function with ProteinMPNN. [PDF]

J Am Chem Soc, 2023
Abstract Natural proteins are highly optimized for function, but are often difficult to produce at a scale suitable for biotechnological applications due to poor expression in heterologous systems, limited solubility, and sensitivity to temperature.
Sumida KH, Núñez-Franco R, Kalvet I, Pellock SJ, Wicky BIM, Milles LF, Dauparas J, Wang J, Kipnis Y, Jameson N, Kang A, De La Cruz J, Sankaran B, Bera AK, Jiménez-Osés G, Baker D.   +15 more
europepmc   +7 more sources

Nanoparticles Affect the Expression Stability of Housekeeping Genes in Plant Cells [PDF]

Nanotechnology, Science and Applications, 2020
Rajendran K Selvakesavan, Gregory Franklin Institute of Plant Genetics of the Polish Academy of Sciences, Poznan 60-479, PolandCorrespondence: Gregory Franklin Tel +48 61 655 0266Email fgre@igr.poznan.plPurpose: We report on the expression stability of ...
Selvakesavan RK, Franklin G
doaj   +2 more sources

Stable Reference Genes for RT‐qPCR in House Dust Mite Dermatophagoides farinae (Acari: Pyroglyphidae) at Different Developmental Stages [PDF]

Ecology and Evolution
To investigate the growth and development mechanisms in Dermatophagoides farinae, it is essential to understand its gene expression profiles across different life stages, which are typically assessed by transcriptional profiling based on RT‐qPCR ...
Feng Jiang, Mingxia Ge, Zeyu Jin, Haoting Zhou, Mengmeng Zhang, Tong Guan, Haoyuan Hu, Jianhai Yin, Ying Liu   +8 more
doaj   +2 more sources

Identification of Reference Genes and Their Application in Analysis of Ginsenoside Biosynthesis Genes in American Ginseng Under Abiotic Stresses and Hormone Treatments [PDF]

Plants
Quantitative real-time PCR (qRT-PCR) is a dominant technique for gene expression analysis in medicinal plants, and accurate qRT-PCR data normalization strictly relies on stably expressed reference genes (RGs).
Fangzhou Zhao, Jiaxin Zhang, Yujuan Zhang, Qingshuai Liu, Debao Huang, Yanwei Zhu, Jinlong Han, Xianchang Wang, Feng Zhang, Shuang Wu, Beijing Tian, Chenggang Shan   +11 more
doaj   +2 more sources

Selection and Validation of Reference Genes for qRT-PCR Analysis of Gene Expression in Tropaeolum majus (Nasturtium)

Horticulturae, 2023
Tropaeolum majus (nasturtium) is an important ornamental and medicinal plant due to its colorful flowers, shield-shaped leaves, and richness in mineral elements and bioactive compounds. However, the key genes related to these important biological traits,
Qing Tang, Guang-Can Zhou, Si-Jie Liu, Wen Li, Yi-Lei Wang, Gao-Ying Xu, Teng-Fei Li, Guo-Qing Meng, Jia-Yu Xue   +8 more
doaj   +1 more source

Evaluation of Reference Genes for Quantitative Real-Time PCR Analysis in the Bean Bug, Riptortus pedestris (Hemiptera: Alydidae)

Insects, 2023
Quantitative real-time PCR (qRT-PCR) is widely accepted as a precise and convenient method for quantitatively analyzing the expression of functional genes. The data normalization strongly depends upon stable reference genes.
Liuyang Wang, Qingyu Liu, Pei Guo, Zhanlin Gao, Dan Chen, Tao Zhang, Jun Ning   +6 more
doaj   +1 more source

Stable Reference Gene Selection for qRT-PCR Normalization in Strawberry (Fragaria × ananassa) Leaves under Different Stress and Light-Quality Conditions

Horticulturae, 2021
Selecting an appropriate reference gene is of crucial importance for improving the accuracy of qRT-PCR analyses. In this study, strawberry (Fragaria ananassa) seedlings were subjected to different environmental conditions including heat, cold, drought ...
Yuntian Ye, Yang Lu, Guangyi Wang, Yongqiang Liu, Yunting Zhang, Haoru Tang   +5 more
doaj   +1 more source

Assessment of Suitable Reference Genes for qRT-PCR Normalization in Eocanthecona furcellata (Wolff)

Insects, 2022
Quantitative reverse transcription–polymerase chain reaction (qRT–PCR) is a widely used tool for measuring gene expression; however, its accuracy relies on normalizing the data to one or more stable reference genes.
Ying-Na Pan, Ru-Na Zhao, Di Fu, Chun Yu, Chun-Ni Pan, Wei Zhou, Wen-Long Chen   +6 more
doaj   +1 more source

Reference Genes for Expression Analysis Using RT-qPCR in Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)

Insects, 2022
Cnaphalocrocis medinalis is a destructive migratory rice pest. Although many studies have investigated its behavioral and physiological responses to environmental changes and migration-inducing factors, little is known about its molecular mechanisms ...
Xiaoyu Zhao, Jiawen Guo, Yanhui Lu, Tianyi Sun, Junce Tian, Jianlei Huang, Hongxing Xu, Zhengliang Wang, Zhongxian Lu   +8 more
doaj   +1 more source

Validation of Potential Reference Genes for Real-Time qPCR Analysis in Pharaoh Ant, Monomorium pharaonis (Hymenoptera: Formicidae)

Frontiers in Physiology, 2022
Ants are highly diverse social insects living in colonies consisted of up to millions of individuals with reproductive division of labors. Due to the interests in disclosing the genetic and epigenetic regulation mechanisms underlying the distinct ...
Guo Ding, Guo Ding, Guo Ding, Qionghua Gao, Qionghua Gao, Jun Chen, Jie Zhao, Guojie Zhang, Guojie Zhang, Guojie Zhang, Weiwei Liu   +10 more
doaj   +1 more source