Results 11 to 20 of about 6,818,443 (298)
A general approach for stabilizing nanobodies for intracellular expression
eLife, 2022 Conventional antibodies and their derived fragments are difficult to deploy against intracellular targets in live cells, due to their bulk and structural complexity.
John G Dingus +4 more
doaj +3 more sources
The effect of oxidation on the stability of G-quadruplex DNA : implications for oncogene expression [PDF]
, 2011 G-quadruplexes (G4-DNA) are a class of secondary structures formed from Guanine rich sequences. In recent years these structures have been implicated in both telomere maintenance and oncogene expression, and have been shown to be abundant in upstream ...
Stebbeds, William Joshua David
core +7 more sources
Stability of expression of Neisseria gonorrhoeae lipooligosaccharides [PDF]
Infection and Immunity, 1986 We compared multiple lipooligosaccharide (LOS) extracts from individual strains of Neisseria gonorrhoeae. Each of the extracts was prepared from single mass cultures grown on solid media under similar conditions but separated by time. We found only subtle variations in the number, electrophoretic mobility, and concentration of components of the LOSs ...
H, Schneider +3 more
openaire +2 more sources
Journal of Integrative Agriculture, 2022 Tomato (Solanumlycopersicum) is a model plant for research on fruit development and stress response, in which gene expression analysis is frequently conducted.
Yao-ke DUAN +6 more
doaj +1 more source
Hprt Serves as an Ideal Reference Gene for qRT-PCR Normalization in Rat DRG Neurons
Journal of Integrative Neuroscience, 2023 Objective: To identify suitable reference genes for gene expression studies in rat dorsal root ganglia (DRG) neurons. Methods: The raw cycle threshold (Ct) values of 12 selected reference genes were obtained via quantitative real-time reverse ...
Fenglan Liu +3 more
doaj +1 more source
Contractile acto-myosin network on nuclear envelope remnants positions human chromosomes for mitosis
eLife, 2019 To ensure proper segregation during mitosis, chromosomes must be efficiently captured by spindle microtubules and subsequently aligned on the mitotic spindle.
Alexander JR Booth +6 more
doaj +1 more source
Frontiers in Physiology, 2021 Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is an accurate and convenient technique for quantifying expression levels of the target genes.
Jiaoxin Xie +7 more
doaj +1 more source
Frontiers in Physiology, 2023 Aphidoletes aphidimyza is a predator that is an important biological agent used to control agricultural and forestry aphids. Although many studies have investigated its biological and ecological characteristics, few molecular studies have been reported ...
Xiu-Xian Shen +7 more
doaj +1 more source
Coronaviruses as Vectors: Stability of Foreign Gene Expression [PDF]
Journal of Virology, 2005 ABSTRACT Coronaviruses are enveloped, positive-stranded RNA viruses considered to be promising vectors for vaccine development, as (i) genes can be deleted, resulting in attenuated viruses; (ii) their tropism can be modified by manipulation of their spike protein; and (iii) heterologous genes can be expressed by simply inserting ...
de Haan, C.A.M. +4 more
openaire +4 more sources
Assessment of suitable reference genes for qRT-PCR analysis in Adelphocoris suturalis
Journal of Integrative Agriculture, 2018 Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is the most commonly-used tool for measurement of gene expression, but its accuracy and reliability depend on appropriate data normalization with the use of one or more stable ...
Jing LUO +8 more
doaj +1 more source