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A Novel Multiplex qRT-PCR Assay to Detect SARS-CoV-2 Infection: High Sensitivity and Increased Testing Capacity [PDF]

open access: yesMicroorganisms, 2020
Rapid and sensitive screening of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential to limit the spread of the global pandemic we are facing.
Rossana Cavallo   +2 more
exaly   +9 more sources

Development of a Multiplex qRT-PCR Assay for Detection of Classical Swine Fever Virus, African Swine Fever Virus, and Erysipelothrix rhusiopathiae [PDF]

open access: yesFrontiers in Veterinary Science, 2023
Classical swine fever virus (CSFV), African swine fever virus (ASFV), and Erysipelothrix rhusiopathiae (E. rhusiopathiae) remain endemic in many parts of China. Co-infections make distinguishing their clinical symptoms and pathological changes difficult.
Xiao-Hui Wen, Dian-Hong Lv, Qi Zhai
exaly   +6 more sources

Development of a Multiplex qRT-PCR Assay for the Detection of Porcine Epidemic Diarrhea Virus, Porcine Transmissible Gastroenteritis Virus and Porcine Deltacoronavirus [PDF]

open access: yesFrontiers in Veterinary Science, 2023
Currently, porcine coronaviruses are prevalent in pigs, and due to the outbreak of COVID-19, porcine coronaviruses have become a research hotspot.
Chun-Ling Li
exaly   +6 more sources

Validation of a Multiplex qRT-PCR Assay for the Detection of RSV, Influenza A/B Virus and SARS-CoV-2 [PDF]

open access: yesBMC Microbiology
The significant burden of viral respiratory diseases necessitates rapid detection of key pathogens. Simultaneous testing for SARS-CoV-2, RSV, and influenza A/B as an initial step, followed by broader panels as needed, offers a cost-effective diagnostic ...
Ayca A Sayiner, Alihan Bulgurcu
exaly   +5 more sources

Development of a Multiplex Real-Time qRT-PCR for Discriminating the Predominant Epidemic Variant IBDV and Very Virulent IBDV [PDF]

open access: yesFrontiers in Veterinary Science
Infectious bursal disease (IBD) is an important immunosuppressive disease of chicken caused by infectious bursal disease virus (IBDV). At present, the newly emerging novel variant IBDV (varIBDV) and the persistently prevalent very virulent IBDV (vvIBDV ...
Ziwen Wu, Runhang Liu, Yulu Duan
exaly   +6 more sources

Development of a one-step multiplex qRT–PCR assay for the detection of African swine fever virus, classical swine fever virus and atypical porcine pestivirus [PDF]

open access: yesBMC Veterinary Research, 2022
Background African swine fever virus (ASFV), classical swine fever virus (CSFV) and atypical porcine pestivirus (APPV) have caused great economic losses to the swine industry in China. Since coinfections of ASFV, CSFV and APPV occur in certain pig herds,
Huixin Liu   +8 more
doaj   +6 more sources

Detection of 22 common leukemic fusion genes using a single-step multiplex qRT-PCR-based assay [PDF]

open access: yesDiagnostic Pathology, 2017
Background Fusion genes generated from chromosomal translocation play an important role in hematological malignancies. Detection of fusion genes currently employ use of either conventional RT-PCR methods or fluorescent in situ hybridization (FISH), where
Xiaodong Lyu   +7 more
doaj   +5 more sources

Development of a Panel of Three Multiplex Allele-Specific qRT-PCR Assays for Quick Differentiation of Recombinant Variants and Omicron Subvariants of SARS-CoV-2

open access: yesFrontiers in Cellular and Infection Microbiology, 2022
Quick differentiation of the circulating variants and the emerging recombinant variants of SARS-CoV-2 is essential to monitor their transmission.
You Yang
exaly   +5 more sources

Development of a Multiplex Crystal Digital RT-PCR for Differential Detection of Classical, Highly Pathogenic, and NADC30-Like Porcine Reproductive and Respiratory Syndrome Virus

open access: yesAnimals, 2023
Porcine reproductive and respiratory syndrome virus (PRRSV) type 1 (European genotype) and PRRSV type 2 (North American genotype) are prevalent all over the world.
Hongbin Si, Kaichuang Shi, Yating Chen
exaly   +3 more sources

A Quadruplex qRT-PCR for Differential Detection of Four Porcine Enteric Coronaviruses

open access: yesVeterinary Sciences, 2022
Porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea syndrome coronavirus (SADS-CoV) are four identified porcine enteric coronaviruses.
Kaichuang Shi, Zongqiang Li, Yanwen Yin
exaly   +3 more sources

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