Results 11 to 20 of about 877,269 (241)

Comprehensive multiplex one-step real-time TaqMan qRT-PCR assays for detection and quantification of hemorrhagic fever viruses. [PDF]

PLoS ONE, 2014
BACKGROUND: Viral hemorrhagic fevers (VHFs) are a group of animal and human illnesses that are mostly caused by several distinct families of viruses including bunyaviruses, flaviviruses, filoviruses and arenaviruses.
Zheng Pang, Aqian Li, Jiandong Li, Jing Qu, Chengcheng He, Shuo Zhang, Chuan Li, Quanfu Zhang, Mifang Liang, Dexin Li   +9 more
doaj   +5 more sources

Detection and Profiling of Diarrheic Marine Biotoxins in Shellfish by mRNA Analysis of Exposed Caco-2 Cells Using qRT-PCR and Multiplex Magnetic Bead-Based Assays

ALTEX: Alternatives To Animal Experimentation, 2019
The mouse bioassay for the detection of marine biotoxins in shellfish products is 40 years old and still in use. A full ban or total replacement of this in vivo test has been postponed because of the fear that current chemical-based detection methods ...
Peter J Hendriksen, Toine F H Bovee
exaly   +6 more sources

Application of Novel Dark-Quencher Labeled Probes in Multiplex qRT-PCR Assays for Rapid Detection of SARS-CoV-2 Variants [PDF]

Journal of Thoracic Disease
Coronavirus disease 2019 (COVID-19) is an acute infectious disease caused by the new coronavirus, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Because SARS-CoV-2 frequently mutates, it creates a number of variants that must be distinguished and tracked using a rapid detection technique.
Kui Zheng, Mengling Jiang, Zhiqi Zeng
exaly   +4 more sources

High Throughput microRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic arrayTM IFCs. [PDF]

Journal of Visualized Experiments, 2011
The broad involvement of miRNAs in critical processes underlying development, tissue homoeostasis and disease has led to a surging interest among the research and pharmaceutical communities. To study miRNAs, it is essential that the quantification of microRNA levels is accurate and robust.
Robert Blelloch
exaly   +5 more sources

Advancing IBDV Diagnostics: A One-Step Multiplex Real-Time qRT-PCR for Discriminating Between vvIBDV and Non-vvIBDV Viruses, Including the Newly Emerged IBDV Variant

Frontiers in Veterinary Science
The very virulent infectious bursal disease virus (vvIBDV) induces an acute, highly contagious and immunosuppressive disease in younger chicken causing massive economic losses globally.
Ahmed Samy, Ali Zanaty, Naglaa Hagag
exaly   +5 more sources

Development of a competition assay to assess the in vitro fitness of dengue virus serotypes using an optimized serotype-specific qRT-PCR. [PDF]

PLoS ONE
BackgroundComparing the in vitro fitness of dengue virus (DENV) isolates is a pivotal approach to assess the contribution of DENV strains' replicative fitness to epidemiological contexts, including serotype replacements.
Anne-Fleur Griffon, Loeïza Rault, Clément Tanvet, Etienne Simon-Lorière, Myrielle Dupont-Rouzeyrol, Catherine Inizan   +5 more
doaj   +2 more sources

Epidemiology of viral and atypical bacterial respiratory infections among children hospitalised at a Kenyan tertiary referral centre: a cross sectional multiplex PCR study [PDF]

Frontiers in Pediatrics
BackgroundSevere acute respiratory infections (SARIs) cause high paediatric morbidity and mortality particularly in sub-Saharan Africa. Understanding contemporary trends in SARI aetiology is needed to inform prevention strategies.
Rohini Kalagouda Patil, Anne Irungu, John Chelanga Supra, Rahul Zode, Koigi Paul Kamau, Joseph K. Mbuthia, Diana Marangu-Boore, Diana Marangu-Boore   +7 more
doaj   +2 more sources

Development of novel AllGlo-probe-based one-step multiplex qRT-PCR assay for rapid identification of avian influenza virus H7N9

Archives of Virology, 2014
Recently, human deaths have resulted from infection with low-pathogenicity avian influenza virus H7N9 strains that have emerged recently in China. To strengthen H7N9 surveillance and outbreak control, rapid and reliable diagnostic methods are needed.
Zhang, Y., Mao, H., Yan, J., Wang, X., Zhang, L., Koch, G., Li, H., Li, Z., Chen, Y., Gong, L., Chen, Z., Xia, S.   +11 more
openaire   +4 more sources

Development of a multiplex qRT-PCR assay for detection of African swine fever virus, classical swine fever virus and porcine reproductive and respiratory syndrome virus. [PDF]

J Vet Sci, 2021
African swine fever virus (ASFV), classical swine fever virus (CSFV), and porcine reproductive and respiratory syndrome virus (PRRSV) are still prevalent in many regions of China. Co-infections make it difficult to distinguish their clinical symptoms and pathological changes.
Chen Y, Shi K, Liu H, Yin Y, Zhao J, Long F, Lu W, Si H.   +7 more
europepmc   +3 more sources

3D microdevices that perform sample purification and multiplex qRT-PCR for early cancer detection with confirmation of specific RNAs. [PDF]

Sci Rep, 2018
Abstract MicroRNA expression analysis is an important screening tool for the early detection of cancer. In this study, we developed two portable three-dimensional microdevices for multiple singleplex RNA expression analysis by microRNA purification and qRT-PCR as a prototype for point-of-care testing.
Kimura Y, Ikeuchi M, Inoue Y, Ikuta K.
europepmc   +4 more sources