Multiplex qRT-PCR for the Detection of Western Equine Encephalomyelitis, St. Louis Encephalitis, and West Nile Viral RNA in Mosquito Pools (Diptera: Culicidae). [PDF]
J Med Entomol, 2015 Following the introduction of West Nile virus into California during the summer of 2003, public health and vector control programs expanded surveillance efforts and were in need of diagnostics capable of rapid, sensitive, and specific detection of arbovirus infections of mosquitoes to inform decision support for intervention. Development of a multiplex
Brault AC, Fang Y, Reisen WK.
europepmc +4 more sources
Differentiating cutaneous squamous cell carcinoma and pseudoepitheliomatous hyperplasia by multiplex qRT-PCR [PDF]
Modern Pathology, 2013 Squamous cell carcinoma is the second most common cutaneous malignancy. The diagnosis can occasionally be difficult as there are many lesions that are mimics, clinically and on pathologic examination. One of the most challenging lesions to differentiate from squamous cell carcinoma is pseudoepitheliomatous hyperplasia, a reactive proliferation of the ...
Albert, Su +4 more
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One-step multiplex real-time RT-PCR assay for detecting and genotyping wild-type group A rotavirus strains and vaccine strains (Rotarix® and RotaTeq®) in stool samples [PDF]
PeerJ, 2016 Background. Group A rotavirus (RVA) infection is the major cause of acute gastroenteritis (AGE) in young children worldwide. Introduction of two live-attenuated rotavirus vaccines, RotaTeq® and Rotarix®, has dramatically reduced RVA associated AGE and ...
Rashi Gautam +5 more
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Frontiers in Veterinary Science, 2022 African swine fever (ASF), classical swine fever (CSF), and porcine reproductive and respiratory syndrome (PRRS) are highly infectious diseases of domestic pigs and wild boars.
Kaichuang Shi +8 more
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Multiplexing primer/probe sets for detection of SARS-CoV-2 by qRT-PCR
Journal of Clinical Virology, 2020 The novel respiratory virus SARS-CoV-2, responsible for over 380,000 COVID-19 related deaths, has caused significant strain on healthcare infrastructure and clinical laboratories globally. The pandemic's initial challenges include broad diagnostic testing, consistent reagent supply lines, and access to laboratory instruments and equipment.
Perchetti, Garrett A. +4 more
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Microfluidic-Based Multiplex qRT-PCR Identifies Diagnostic and Prognostic microRNA Signatures in the Sera of Prostate Cancer Patients [PDF]
Cancer Research, 2011 Abstract Recent prostate-specific antigen–based screening trials indicate an urgent need for novel and noninvasive biomarker identification strategies to improve the prediction of prostate cancer behavior. Noncoding microRNAs (miRNA) in the serum and plasma have been shown to have potential as noninvasive markers for physiologic and ...
Moltzahn, Felix +9 more
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Forensic Body Fluid Identification by Analysis of Multiple RNA Markers Using NanoString Technology [PDF]
Genomics & Informatics, 2013 RNA analysis has become a reliable method of body fluid identification for forensic use. Previously, we developed a combination of four multiplex quantitative PCR (qRT-PCR) probes to discriminate four different body fluids (blood, semen, saliva, and ...
Jong-Lyul Park +6 more
doaj +1 more source
Detection of Brucella organisms from Egyptian raw milk using cultural and molecular techniques [PDF]
Kafrelsheikh Veterinary Medical Journal, 2020 Aim: This study aimed to detect Brucella organisms in Egyptian raw milk using cultural and molecular techniques.Methods: Two hundred bulk milk samples were collected from 2 cities at El-Gharbia and Kafer El-Sheikh Governorates during the summer and ...
Ahmed EL-Gushi +3 more
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Iranian Journal of Parasitology, 2020 Background: Malaria is threatening more than half of Afghanistan population. Asymptomatic malaria is notable problem against malaria controlling strategies.
Sayed Hussain MOSAWI +4 more
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