Results 71 to 80 of about 1,108,829 (262)

qRT-PCR amplification of viral RNA.

, 2021
Methods from the experiment shown in S4 Fig. After centrifugation, 200 μl of supernatant were extracted using the « High Pure Viral Nucleic Acid » kit from Roche following the supplier’s recommendations. qRT-PCR was performed following indications of the
Paul O. Verhoeven (9155079), Gérard Baldacchino (11401976), Simon Hemour (11401979), Wahbi Jomaa (11401973), Fréderic Becquart (11401970), Marie-Line Andreola (230436)   +5 more
core   +1 more source

Relationship Between Neurologic Symptoms and Signs and FMR1 Genotype in Premutation Carriers

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background and Objectives Fragile X‐associated Tremor/Ataxia Syndrome (FXTAS) is the most severe late‐onset condition caused by a premutation in the FMR1 gene, characterized by expanded CGG triplet repeats of 55–200. Clinical presentations of FXTAS, including gait ataxia, kinetic tremor, cognitive decline, and rare Parkinsonism, are linked to ...
Flora Tassone, Freddy Chafota, Miguel E. Rentería, Randi J. Hagerman, Ellery Santos, Anna Atkinson, Nicholas G. Martin, Elsdon Storey, Danuta Z. Loesch   +8 more
wiley   +1 more source

qRT-PCR validation for 9 candidate transcripts.

, 2017
Log2FC means log2 fold change in RNA-Seq experiment while RNE means relative normalized expression (2-ΔΔCt) in qRT-PCR experiment. Error bar represents the standard deviation.
Jianhua Miao (3530219), Changming Mo (3611324), Longhua Bai (3530222), Shugen Wei (3611318), Zhonglian Zhang (3611321), Hao Huang (111923), Limei Pan (3611330), Jine Fu (3611333), Yanhong Guan (3611327), Xiaojun Ma (231882), Maoshan Chen (194424)   +10 more
core   +1 more source

Integrated PANoptosis Profiling Identifies Immunosuppressive Subtypes and a Prognostic Signature With Functional Validation of MLKL in Glioblastoma

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective The prognosis of glioblastoma (GBM) remains highly unfavorable, largely due to high tumor heterogeneity and an immunosuppressive microenvironment. However, the functional role of PANoptosis in this context is poorly understood. Methods Patients were stratified via K‐means clustering. A risk score model was constructed using prognosis‐
Langfei Tian, Minghui Zhao, Yuanbo Hu, Kaiyue Wang, Haiguang Liu, Zetong Bai, Kebin Zheng   +6 more
wiley   +1 more source

qRT-PCR validation of candidate genes.

, 2017
Four candidate genes were chosen from each of the epidermis and dermis for validation by qRT-PCR. The bar graphs represent the qRT-PCR data for the microdissected keloid sites and normal skin and the line graph represents the associated microarray fold ...
T. Hodgkinson (3812695), R. Paus (3812698), N. Jumper (3812692), A. Bayat (3812689)   +3 more
core   +1 more source

Patient‐Derived 3D Heart‐On‐a‐Chip Model of Dilated Cardiomyopathy With Embedded Bead‐Based Mapping of Tissue Contractility

Advanced Healthcare Materials, EarlyView.
A heart‐on‐a‐chip model of dilated cardiomyopathy is developed from patient‐derived induced pluripotent stem cells. The model recapitulates key disease phenotypes and enables functional assessment through integrated bead‐based tracking and pillar deflection measurements.
Ali Mousavi, Ludovic Mouttet, Shihao Cui, Yasaman Hekmatnia, Mehran Mottahedi, Ida Derish, Naimeh Rafatian, Mark Aurousseau, Gregor Andelfinger, Renzo Cecere, Houman Savoji   +10 more
wiley   +1 more source

Spatially Controlled Embedded Bioprinting of Prevascularized Spheroids‐Containing Biopatch Enables Functional Skin Regeneration

Advanced Healthcare Materials, EarlyView.
A spatially controlled embedded bioprinting strategy enables precise engineering of prevascularized multicellular spheroids within skin‐derived dECM bioinks. Optimized spheroid size, composition, and spacing promote vascular maturation, paracrine signaling, and tissue integration, resulting in enhanced angiogenesis, blood perfusion, and functional skin
Minjun Ahn, Gyu Tae Park, Dongjun Lee, Sik Yoon, Sae‐Ock Oh, Jae Ho Kim, Byoung Soo Kim   +6 more
wiley   +1 more source

SAGE and quantitative PCR (qRT-PCR) analysis of select genes: (A) Genes found to have reversible expression upon smoking cessation

, 2011
Copyright information:Taken from "Effect of active smoking on the human bronchial epithelium transcriptome"http://www.biomedcentral.com/1471-2164/8/297BMC Genomics 2007;8():297-297.Published online 29 Aug 2007PMCID:PMC2001199.
Kim M Lonergan (31722), Raj Chari (23305), Calum MacAulay (2879), Wan L Lam (2880), Stephen Lam (61568), Raymond T Ng (62483)   +5 more
core   +1 more source

Engineered Nanoplatforms Targeting Activated Hepatic Stellate Cells for Liver Fibrosis Therapy via TGF‐β Axis Inhibition and Ferroptosis Induction

Advanced Healthcare Materials, EarlyView.
FeS‐VA@lanifibranor (FVL), an activated hepatic stellate cell (aHSC)‐targeted nanoplatform comprising vitamin A‐functionalized two‐dimensional iron sulfide nanosheets and lanifibranor, is engineered for precision therapy of liver fibrosis. FVL suppresses the TGF‐β signaling pathway by inhibiting SMAD2/3 phosphorylation and induces ferroptosis in aHSCs,
Yinuo Yang, Mengyao Mu, Ke Ren, Hui Liu, Pengju Ma, Yingqi Wang, Mengmeng Zhang, Xueli Xu, Wenzhe Li, Wenshuang Gao, Xiao Sun, Qiang Zhu   +11 more
wiley   +1 more source

qRT-PCR validation of DEGs identified by RNA-Seq.

, 2018
Sixteen DEGs involved in the biosynthesis and metabolism of indican were randomly selected for qRT-PCR confirmation. The blue bars and red lines represent RNA-Seq RPKM data and qRT-PCR relative expression levels, respectively.
Shuju Ning (5484932), Wei Huang (36889), Xiaohua Wang (255149), Daozhi Wei (5484926), Wenjin Lin (5484929), Qi Ye (243032)   +5 more
core   +1 more source