Results 61 to 70 of about 1,108,829 (262)
qRT-PCR primers and associated genes.
, 2016 qRT-PCR primers and associated genes.
Michael A. Cotten (2634094) +3 more
core +1 more source
qRT-PCR validation of mRNA expression changes.
, 2023 (a, b) A subset of tested genes were selected for validation using quantitative Reverse Transcription–Polymerase Chain Reaction (qRT-PCR). 8 genes were selected for a temporal analysis from 0-72h post Mtz in either the NTR-rod (A) or NTR-bipolar (B ...
David T. White (5648471) +12 more
core +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
qRT-PCR validation of the differentially expressed miRNAs and mRNAs.
, 2018 A: Comparison of the mRNA sequencing and qRT-PCR results. B: Comparison of the miRNA sequencing and qRT-PCR results.
Na Li (6550) +8 more
core +1 more source
FEBS Open Bio, EarlyView.The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source
qRT-PCR validation of RNA-seq results.
, 2016 qRT-PCR validation of RNA-seq results.
Li Kang (494757) +9 more
core +1 more source
BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells
FEBS Open Bio, EarlyView.BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley +1 more source
qRT-PCR primers used in this study.
, 2017 qRT-PCR primers used in this study.
Karoline Maria Vieira Nogueira (3686413) +8 more
core +1 more source
MARK4 enhances stress granule formation under oxidative stress and increases tau accumulation
FEBS Open Bio, EarlyView.MARK4 (red dots) localizes to stress granules (orange dots) and promotes their formation under oxidative stress by modulating TIA1 (blue dots). MARK4 and TIA1 synergistically increase tau (purple) accumulation, and the reduction of the TIA1 ortholog suppresses neurodegeneration in a fly model.
Sho Nakajima +8 more
wiley +1 more source
QRT-PCR validation of 7 miRNAs.
, 2018 (A) The small RNA-Seq log2 value (expression ratios of S03-RPKM/S04-RPKM) and the qRT-PCR log2 value (expression ratios of S03/S04) of three important miRNAs (bna-miR156e, conservative-chrA05-439469 and unconservative-chrCnn-random-3253054) which the ...
Zhipeng Niu (3519488) +7 more
core +1 more source