Results 31 to 40 of about 494,714 (299)

Detection of ROS1 gene rearrangement in lung adenocarcinoma: comparison of IHC, FISH and real-time RT-PCR. [PDF]

PLoS ONE, 2015
AIMS:To compare fluorescence in situ hybridization (FISH), immunohistochemistry (IHC) and quantitative real-time reverse transcription-PCR (qRT-PCR) assays for detection of ROS1 fusion in a large number of ROS1-positive lung adenocatcinoma (ADC) patients.
Ling Shan, Fang Lian, Lei Guo, Tian Qiu, Yun Ling, Jianming Ying, Dongmei Lin   +6 more
doaj   +1 more source

RNA interference in marine and freshwater sponges [PDF]

, 2011
Background: The marine sponge Tethya wilhelma and the freshwater sponge Ephydatia muelleri are emerging model organisms to study evolution, gene regulation, development, and physiology in non-bilaterian animal systems. Thus far, functional methods (i.e.,
Cieniewicz, Brandon, Danka, Elizabeth S., Haen, Karri M., Hammel, Jörg U., Hill, April L., Hill, Malcolm S., Knight, Scott W., Lavrov, Dennis V., Nickel, Michael, Posfai, Dora, Rivera, Ajna S., Winters, Ian P., Wörheide, Gert   +12 more
core   +1 more source

Her2 assessment using quantitative reverse transcriptase polymerase chain reaction reliably identifies Her2 overexpression without amplification in breast cancer cases

Journal of Translational Medicine, 2017
Background Immunohistochemistry (IHC) and fluorescent-in situ hybridization (FISH) are standard methods to assess human epidermal growth factor receptor 2 (HER2) status in breast cancer (BC) patients. Real-time quantitative polymerase-chain-reaction (qRT-
Gabriele Zoppoli, Anna Garuti, Gabriella Cirmena, Ludovica Verdun di Cantogno, Cristina Botta, Maurizio Gallo, Domenico Ferraioli, Enrico Carminati, Paola Baccini, Monica Curto, Piero Fregatti, Edoardo Isnaldi, Michela Lia, Roberto Murialdo, Daniele Friedman, Anna Sapino, Alberto Ballestrero   +16 more
doaj   +1 more source

ER and HER2 expression are positively correlated in HER2 non-overexpressing breast cancer [PDF]

, 2012
PMCID: PMC3446380This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided ...
A Borg, AC Wolff, Adrian Harris, C Wright, DA Budwit-Novotny, DJ Slamon, DJ Slamon, E Tagliabue, EA Musgrove, EHR Perez, G Arpino, G Konecny, GD Lewis Phillips, Georges Sumo, IL Andrulis, Isabel Pinhel, Ivana Sestak, J Cuzick, J Vandesompele, JA Sparano, Jack Cuzick, Janine Salter, JC Brase, JM Harvey, Judith M Bliss, M Dowsett, M Dowsett, M Harigopal, Margaret Hills, ME Hammond, MF Press, Mitch Dowsett, MJ Ellis, MJ Ellis, MJ Piccart-Gebhart, N Quenel, P Royston, Peter Barrett-Lee, RL Camp, Roger A'Hern, S Drury, S Johnston, S Paik, SJ Houston, SS Badve, Suzanne Drury, The Adjuvant Breast Cancer Trials Collaborative Group, V Guarneri, VS Perez   +48 more
core   +5 more sources

Development of a panel of three multiplex allele-specific qRT-PCR assays for quick differentiation of recombinant variants and Omicron subvariants of SARS-CoV-2

Frontiers in Cellular and Infection Microbiology, 2022
Quick differentiation of the circulating variants and the emerging recombinant variants of SARS-CoV-2 is essential to monitor their transmission.
Jianguo Li, Zefeng Gao, Jing Chen, Ruiling Cheng, Jiahui Niu, Jialei Zhang, You Yang, Ximei Yuan, Juan Xia, Guoli Mao, Hulong Liu, Yongkang Dong, Changxin Wu   +12 more
doaj   +1 more source

Expression of μ-protocadherin is negatively regulated by the activation of the β-catenin signaling pathway in normal and cancer colorectal enterocytes. [PDF]

, 2016
Mu-protocadherin (MUCDHL) is an adhesion molecule predominantly expressed by colorectal epithelial cells which is markedly downregulated upon malignant transformation.
Calabretta, Bruno, Ferrari, Stefano, Ferrarini, Fabrizio, Gemelli, Claudia, Grande, Alexis, Losi, Lorena, Manco, Gianrocco, Montorsi, Lucia, Parenti, Sandra, Rossi, A, Tagliafico, Enrico, Zanocco-Marani, Tommaso   +11 more
core   +2 more sources

Evaluation of bacterial internalization using qRT-PCR [PDF]

GERMS, 2014
Bacterial resistance to antibiotics currently represents a topic of high interest. Unfortunately, studies addressing their adaptation and multidrug tolerance latency1 are not sufficient for a comprehensive description of the pathogen-host molecular interaction mechanisms (interactome) and the bacterial internalization into human cells, which leads to ...
openaire   +2 more sources

RNA isolation and qRT-PCR v1

, 2023
This protocol describes the RNA isolation from cultured cells and quantitative RT PCR.
Narayana Yadavalli, Shawn M. Ferguson
openaire   +1 more source

Drought tolerance conferred to sugarcane by association with Gluconacetobacter diazotrophicus: a transcriptomic view of hormone pathways [PDF]

, 2014
Sugarcane interacts with particular types of beneficial nitrogen-fixing bacteria that provide fixed-nitrogen and plant growth hormones to host plants, promoting an increase in plant biomass.
de Carvalho, Thais G, Farrinelli, Laurent, Ferreira, Paulo CG, Hemerly, Adriana S, Mota Filho, José P, Rojas, Cristian A, Santa Brigida, Ailton B, Van Bel, Michiel, Vandepoele, Klaas, Vaneechoutte, Dries, Vargas, Lívia   +10 more
core   +4 more sources

Assessment of mRNA Splice Variants by qRT-PCR [PDF]

, 2013
Alternative splicing is an essential process for the generation of protein diversity. The physiological role, cellular localization, and abundance of splice variant products compared to the wild-type protein may be completely different. This is illustrated by the five splice variants of the antiapoptotic protein survivin that are more abundant in ...
Ileabett M Echevarria, Vargas, Pablo E, Vivas-Mejía   +1 more
openaire   +2 more sources