Results 31 to 40 of about 1,108,829 (262)

Development of a qRT - PCR Diagnostic Kit for the Detection of FCoV

Cyprus Journal of Medical Sciences
BACKGROUND/AIMS: In recent years, feline infectious peritonitis (FIP), which led to the death of more than 3,000 cats on the island of Cyprus, has become a significant concern. FIP is a fatal disease in cats caused by feline coronavirus (FCoV).
Gülten Tuncel Dereboylu, Gökçe Akan, Melis Kalaycı, Cenk Serhan Özverel, Tamer Şanlıdağ   +4 more
doaj   +1 more source

Evaluation of the performance of a multiplex reverse transcription polymerase chain reaction kit as a potential diagnostic and surveillance kit for rotavirus in Kenya

Tropical Diseases, Travel Medicine and Vaccines, 2019
Background Diarrhea is a serious concern worldwide, especially in developing countries. Rotavirus is implicated in approximately 400,000 infant deaths annually.
Cliff Odhiambo Philip, Margaret Koech, Nancy Kipkemoi, Ronald Kirera, Janet Ndonye, Abigael Ombogo, Mary Kirui, Erick Kipkirui, Brook Danboise, Christine Hulseberg, Stacey Bateman, Alexander Flynn, Brett Swierczewski, Esther Magiri, Elizabeth Odundo   +14 more
doaj   +1 more source

Analytical comparisons of SARS-COV-2 detection by qRT-PCR and ddPCR with multiple primer/probe sets

Emerging Microbes and Infections, 2020
Different primers/probes sets have been developed all over the world for the nucleic acid detection of SARS-CoV-2 by quantitative real time polymerase chain reaction (qRT-PCR) as a standard method.
Xinjin Liu, Jiangpeng Feng, Qiuhan Zhang, Dong Guo, Lu Zhang, Tao Suo, Wenjia Hu, Ming Guo, Xin Wang, Zhixiang Huang, Yong Xiong, Guozhong Chen, Yu Chen, Ke Lan   +13 more
doaj   +1 more source

M gene targeted qRT-PCR approach for SARS-CoV-2 virus detection

Scientific Reports, 2023
Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) is the gold standard method for SARS-CoV-2 detection, and several qRT-PCR kits have been established targeting different genes of the virus.
Md. Murshed Hasan Sarkar, Showti Raheel Naser, Sanjana Fatema Chowdhury, Md. Salim Khan, Md. Ahashan Habib, Shahina Akter, Tanjina Akhtar Banu, Barna Goswami, Iffat Jahan, Maksudur Rahman Nayem, Md. Akibul Hassan, Md. Imran Khan, Mohammad Fazle Alam Rabbi, Chowdhury Rafiqul Ahsan, Md. Ibrahim Miah, Afzalun Nessa, S. M. Rashed Ul Islam, Mohammed Atiqur Rahman, Md. Aftab Ali Shaikh, Md. Sharfuddin Ahmed   +19 more
doaj   +1 more source

qRT-PCR primers.

, 2017
qRT-PCR primers.
Qian Mu (779498), Haifeng Jia (3296727), Kekun Zhang (3694714), Xiang Fang (147347), Yiqun Bao (2602384), Jinggui Fang (211817), Lingfei Shangguan (211786), Xiaoying Li (147528)   +7 more
core   +1 more source

Detection of 22 common leukemic fusion genes using a single-step multiplex qRT-PCR-based assay

Diagnostic Pathology, 2017
Background Fusion genes generated from chromosomal translocation play an important role in hematological malignancies. Detection of fusion genes currently employ use of either conventional RT-PCR methods or fluorescent in situ hybridization (FISH), where
Xiaodong Lyu, Xianwei Wang, Lina Zhang, Zhenzhu Chen, Yu Zhao, Jieying Hu, Ruihua Fan, Yongping Song   +7 more
doaj   +1 more source

Reference gene selection for quantitative real-time PCR (qRT-PCR) expression analysis in Galium aparine L.

PLoS ONE, 2020
To accurately evaluate expression levels of target genes, stable internal reference genes is required for normalization of quantitative real-time PCR (qRT-PCR) data.
Xu Su, Liuyang Lu, Yashe Li, Congai Zhen, Guilei Hu, Kun Jiang, Yawei Yan, Yanbo Xu, Geng Wang, Mingwang Shi, Xiling Chen, Baizhong Zhang   +11 more
doaj   +1 more source

One-step multiplex real-time RT-PCR assay for detecting and genotyping wild-type group A rotavirus strains and vaccine strains (Rotarix® and RotaTeq®) in stool samples [PDF]

PeerJ, 2016
Background. Group A rotavirus (RVA) infection is the major cause of acute gastroenteritis (AGE) in young children worldwide. Introduction of two live-attenuated rotavirus vaccines, RotaTeq® and Rotarix®, has dramatically reduced RVA associated AGE and ...
Rashi Gautam, Slavica Mijatovic-Rustempasic, Mathew D. Esona, Ka Ian Tam, Osbourne Quaye, Michael D. Bowen   +5 more
doaj   +2 more sources

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

FEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg, Martine Hjeltnes, Josephine Krieger, Joshua E. Elias, Maria Stensland, Kristian Holm, Xiaojun Jiang, Chuki Khangsar, Tuula A. Nyman, Arthur Kaser, Johannes R. Hov, Tom H. Karlsen, Nicole C. Kaneider, Frode L. Jahnsen, Georg Schneditz   +14 more
wiley   +1 more source

Standardization of Gene Expression Quantification by Absolute Real-Time qRT-PCR System Using a Single Standard for Marker and Reference Genes

Biomarker Insights, 2010
In the last decade, genome-wide gene expression data has been collected from a large number of cancer specimens. In many studies utilizing either microarray-based or knowledge-based gene expression profiling, both the validation of candidate genes and ...
Yi-Hong Zhou, Vinay R. Raj, Eric Siegel, Liping Yu   +3 more
doaj   +2 more sources