Results 21 to 30 of about 1,108,829 (262)

qRT-PCR using standard curves.

, 2021
(A) qRT-PCR standard curves for the individual standard RNAs. The molar amount of the respective RNA fragment introduced into the qRT-PCR reaction is given on the x-axis in logarithmic scale.
Nadine Biedenkopf (2834705), Lennart Kämper (11608114), Stephan Becker (146493), Roland K. Hartmann (9320167), Marcus Lechner (618023), Paul Klemm (6262502), Jana-Christin Demper (9339743), Julia Schlereth (2834699), Simone Bach (9339740), Friedemann Weber (45518), Andreas Schoen (11608111)   +10 more
core   +1 more source

No control genes required: Bayesian analysis of qRT-PCR data.

PLoS ONE, 2013
BackgroundModel-based analysis of data from quantitative reverse-transcription PCR (qRT-PCR) is potentially more powerful and versatile than traditional methods.
Mikhail V Matz, Rachel M Wright, James G Scott   +2 more
doaj   +1 more source

qRT-PCR analysis.

, 2023
Randomly selected eight differential genes and calculated relative gene expressions by qRT-PCR using the 2-ΔΔCt method. Bar graphs indicate the fold difference in transcript levels between the sisal and control under Cd stress; * indicates ...
Tao Chen (75911), Shibei Tan (7470635), Kexian Yi (652533), Jing Li (10611), Weihuai Wu (6896720), Lifang Ge (13907871), Helong Chen (652531), Jingen Xi (652538), Xing Huang (129439), Zhaoxue Huang (17479295), Ping Liu (156158)   +10 more
core   +1 more source

Comparison of 2 Assays for Diagnosing Rotavirus and Evaluating Vaccine Effectiveness in Children with Gastroenteritis

Emerging Infectious Diseases, 2013
We compared rotavirus detection rates in children with acute gastroenteritis (AGE) and in healthy controls using enzyme immunoassays (EIAs) and semiquantitative real-time reverse transcription PCR (qRT-PCR).
Jacqueline E. Tate, Slavica Mijatovic-Rustempasic, Ka Ian Tam, Freda C. Lyde, Daniel C. Payne, Peter Szilagyi, Kathryn Edwards, Mary Allen Staat, Geoffrey A. Weinberg, Caroline B. Hall, James Chappell, Monica McNeal, Jon R. Gentsch, Michael D. Bowen, Umesh D. Parashar   +14 more
doaj   +1 more source

Detection of ROS1 gene rearrangement in lung adenocarcinoma: comparison of IHC, FISH and real-time RT-PCR. [PDF]

PLoS ONE, 2015
AIMS:To compare fluorescence in situ hybridization (FISH), immunohistochemistry (IHC) and quantitative real-time reverse transcription-PCR (qRT-PCR) assays for detection of ROS1 fusion in a large number of ROS1-positive lung adenocatcinoma (ADC) patients.
Ling Shan, Fang Lian, Lei Guo, Tian Qiu, Yun Ling, Jianming Ying, Dongmei Lin   +6 more
doaj   +1 more source

qRT-PCR verification of differential genes.

, 2022
qRT-PCR verification of differential genes.
Genying Fu (13804656), Chuang Huang (4520347), Shuang Yu (181261), Zhiwei Wang (120259), Peixia Sun (13804653), Liping Zhang (177396), Wei Zhang (405), Pingwu Liu (12697711), Dan Wu (5969), Shanhan Cheng (8894479), Guopeng Zhu (8545638), Qin Deng (3224841)   +11 more
core   +1 more source

Her2 assessment using quantitative reverse transcriptase polymerase chain reaction reliably identifies Her2 overexpression without amplification in breast cancer cases

Journal of Translational Medicine, 2017
Background Immunohistochemistry (IHC) and fluorescent-in situ hybridization (FISH) are standard methods to assess human epidermal growth factor receptor 2 (HER2) status in breast cancer (BC) patients. Real-time quantitative polymerase-chain-reaction (qRT-
Gabriele Zoppoli, Anna Garuti, Gabriella Cirmena, Ludovica Verdun di Cantogno, Cristina Botta, Maurizio Gallo, Domenico Ferraioli, Enrico Carminati, Paola Baccini, Monica Curto, Piero Fregatti, Edoardo Isnaldi, Michela Lia, Roberto Murialdo, Daniele Friedman, Anna Sapino, Alberto Ballestrero   +16 more
doaj   +1 more source

qRT-PCR probes.

, 2020
qRT-PCR probes.
Charles E. Murry (127925), Jill J. Weyers (8313435), Timothy C. Cox (8313453), Hans Reinecke (247331), Amy Martinson (8313444), William M. Mahoney (8313447), Benjamin Van Biber (8313441), Stephen M. Schwartz (8313450), Jagadambika J. Gunaje (8313438)   +8 more
core   +1 more source

Development qRT-PCR Protocol to Predict Strawberry Fusarium Wilt Occurrence

The Plant Pathology Journal, 2018
Strawberry Fusarium wilt disease, caused by Fusarium oxysporum f. sp. fragariae, is the most devastating disease in strawberry production. The pathogen produces chlamydospores which tolerate against harsh environment, fungicide and survive for decades in
Sung Won Hong, Da-Ran Kim, Ji Su Kim, Gyeongjun Cho, Chang Wook Jeon, Youn-Sig Kwak   +5 more
doaj   +1 more source

Development of a panel of three multiplex allele-specific qRT-PCR assays for quick differentiation of recombinant variants and Omicron subvariants of SARS-CoV-2

Frontiers in Cellular and Infection Microbiology, 2022
Quick differentiation of the circulating variants and the emerging recombinant variants of SARS-CoV-2 is essential to monitor their transmission.
Jianguo Li, Zefeng Gao, Jing Chen, Ruiling Cheng, Jiahui Niu, Jialei Zhang, You Yang, Ximei Yuan, Juan Xia, Guoli Mao, Hulong Liu, Yongkang Dong, Changxin Wu   +12 more
doaj   +1 more source