Results 21 to 30 of about 494,714 (299)

Comparative analysis of the diagnostic performance of five commercial COVID-19 qRT PCR kits used in India

Scientific Reports, 2021
To meet the unprecedented requirement of diagnostic testing for SARS-CoV-2, a large number of diagnostic kits were authorized by concerned authorities for diagnostic use within a short period of time during the initial phases of the ongoing pandemic.
J. Singh, A. K. Yadav, A. Pakhare, P. Kulkarni, L. Lokhande, P. Soni, M. Dadheech, P. Gupta, N. Masarkar, A. K. Maurya, S. Nema, D. Biswas, S. Singh   +12 more
doaj   +1 more source

Gene expression and splicing alterations analyzed by high throughput RNA sequencing of chronic lymphocytic leukemia specimens. [PDF]

, 2015
BackgroundTo determine differentially expressed and spliced RNA transcripts in chronic lymphocytic leukemia specimens a high throughput RNA-sequencing (HTS RNA-seq) analysis was performed.MethodsTen CLL specimens and five normal peripheral blood CD19+ B ...
A Ameur, A Choudhury, A Hidalgo, A Rosenwald, AS Kamiguti, C Haslinger, C Soo, C Trapnell, C Trapnell, CJ David, DC Gilby, E Moon, E Moravcikova, F Colotta, F Talab, FC Kelleher, Gwen Jordaan, H Dohner, H Jiang, J Eswaran, J Vilpo, JC Marioni, JJ Dongen van, JP Venables, K Inada, K Zen, L Chen, L Wang, L Yang, LQ Zhang, M Crespo, M Rehli, M Shinohara, M Sultan, Matteo Pelegrini, ML Hermiston, MW Pfaffl, N Chiorazzi, NA Twine, O Bermudez, P Guglielmi, P Kapranov, PG Ferreira, Phillipp Nham, Q Huang, RA Higgins, RI Fernando, RK Gandhirajan, RN Damle, Ryan T. Phan, S Anders, S Courtois, S Decker, S Falt, S Ibrahim, S Liu, S Ma, S Ren, S Shen, Sanjai Sharma, SM Russell, SP Shah, SR Gunn, T Ormsby, TJ Hamblin, U Klein, V Quesada, Wei Liao, XS Puente, Y Pawitan, Y Pekarsky, Y Yu, Z Wang   +72 more
core   +1 more source

EVALUACIÓN DE LA EXPRESIÓN DE GENES IMPLICADOS EN LA BIOSÍNTESIS DE ALMIDÓN EN DIFERENTES VARIEDADES DE YUCA

Acta Biológica Colombiana, 2015
Las raíces almacenadoras de yuca representan una fuente importante de almidón. La ruta metabólica del almidón ha sido reconstruida recientemente en yuca gracias a la liberación de la secuencia completa de su genoma. En este estudio se evaluó la expresión
Simón Pedro Cortés Sierra, Paul Chavarriaga, Hernán Ceballos, Camilo Ernesto López Carrascal   +3 more
doaj   +1 more source

Serum microRNA array analysis identifies miR-140-3p, miR-33b-3p and miR-671-3p as potential osteoarthritis biomarkers involved in metabolic processes. [PDF]

, 2017
Background: MicroRNAs (miRNAs) in circulation have emerged as promising biomarkers. In this study, we aimed to identify a circulating miRNA signature for osteoarthritis (OA) patients and in combination with bioinformatics analysis to evaluate the utility
A Brazma, A Nan, A Okuhara, A Turchinovich, B Aryal, B Mayr, B Sharif, B Zhang, BM Bolstad, BM Kuhn, BM Mayr, C Beyer, C Chen, C Guay, C Taurino, CL Andersen, D Dlouha, D Iliopoulos, D Zhang, DT Felson, F Kostopoulou, FE Nicolas, G Dennis Jr, G Li, G Tardif, GD Miles, GR Sondag, GS Lien, H Causton, JB van Meurs, JD Storey, JD Storey, KC Vickers, L Qi, M Cross, M Parrizas, MB Goldring, MC Hochberg, MD Paraskevopoulou, MP Gantier, OF Laterza, Q Zhuo, R Gernapudi, RF Loeser, RI Gregory, S Kluzek, S Kumar, S Miyaki, T Thomou, TA Karlsen, VM Borgonio Cuadra, W Cleveland, WM Reichmann, X Chen, X Chen, X Tan, X Wang, Y Klipper-Aurbach, Y Zhang, YH Li   +59 more
core   +2 more sources

RNA-seq reveals post-transcriptional regulation of Drosophila insulin-like peptide dilp8 and the neuropeptide-like precursor Nplp2 by the exoribonuclease Pacman/XRN1 [PDF]

, 2015
Ribonucleases are critically important in many cellular and developmental processes and defects in their expression are associated with human disease.
Amy L. Pashler, Benjamin P. Towler, Christopher I. Jones, Kannan, Sarah F. Newbury, Sophie R. Robinson   +5 more
core   +1 more source

Covid-19 Detection using qRT-PCR - A Review

Journal for Research in Applied Sciences and Biotechnology, 2022
In the ongoing Covid pandemic, the monitoring of SARSnCoV-2 with the help of viral loads/viral kinetics has become more essential via the RT-PCR technique. However, the interpretations of qRT-PCR technique results are made as qualitative and quantitative or semi-quantitative, and day by day, this interpretation is becoming more important.
Yagyavalkya Sharma, Lalita Sharma, Priya Agrawal, Megha Gupta, Harsh Vardhan   +4 more
openaire   +1 more source

The biogenesis pathway of tRNA-derived piRNAs in Bombyx germ cells. [PDF]

, 2017
Transfer RNAs (tRNAs) function in translational machinery and further serves as a source of short non-coding RNAs (ncRNAs). tRNA-derived ncRNAs show differential expression profiles and play roles in many biological processes beyond translation ...
Honda, Shozo, Kawamura, Takuya, Kirino, PhD, Yohei, Loher, Phillipe, Morichika, Keisuke, Rigoutsos, Isidore   +5 more
core   +2 more sources

Selection and validation of reference genes for qRT‐PCR analysis during fruit ripening of red pitaya (Hylocereus polyrhizus)

FEBS Open Bio, 2021
Red pitaya (Hylocereus polyrhizus) is widely cultivated in southern and southwestern China. To provide a basis for studying the molecular mechanisms of the ripening of this fruit, we carried out RNA sequencing (RNA‐seq) analysis to identify ...
Qianming Zheng, Xiaoke Wang, Yong Qi, Yuhua Ma   +3 more
doaj   +1 more source

Hemojuvelin-Neogenin Interaction Is Required for Bone Morphogenic Protein-4-induced Hepcidin Expression [PDF]

, 2009
Hemojuvelin (HJV) is a glycosylphosphatidylinositol-linked protein and binds both bone morphogenic proteins (BMPs) and neogenin. Cellular HJV acts as a BMP co-receptor to enhance the transcription of hepcidin, a key iron regulatory hormone secreted ...
An-Sheng Zhang, Andriopoulos, Babitt, Babitt, Babitt, Camaschella, Caroline A. Enns, Conrad, Davies, De Domenico, De Gobbi, Du, Fan Yang, Feng, Feng, Finberg, Frazer, Goldschneider, Hentze, Hidekazu Tsukamoto, Huang, Jiaohong Wang, Kautz, Kuns-Hashimoto, Lin, Lin, Luxon, Matsunaga, Matsunaga, Maxson, Meynard, Niederkofler, Papanikolaou, Pigeon, Samad, Schaffar, Silvestri, Wang, Xia, Xia, Yang, Zhang, Zhang, Zhang, Zhang   +44 more
core   +4 more sources

Comparison of 2 Assays for Diagnosing Rotavirus and Evaluating Vaccine Effectiveness in Children with Gastroenteritis

Emerging Infectious Diseases, 2013
We compared rotavirus detection rates in children with acute gastroenteritis (AGE) and in healthy controls using enzyme immunoassays (EIAs) and semiquantitative real-time reverse transcription PCR (qRT-PCR).
Jacqueline E. Tate, Slavica Mijatovic-Rustempasic, Ka Ian Tam, Freda C. Lyde, Daniel C. Payne, Peter Szilagyi, Kathryn Edwards, Mary Allen Staat, Geoffrey A. Weinberg, Caroline B. Hall, James Chappell, Monica McNeal, Jon R. Gentsch, Michael D. Bowen, Umesh D. Parashar   +14 more
doaj   +1 more source