Veterinary Sciences, 2023 A duplex real-time quantitative reverse transcription-polymerase chain reaction (dqRT-PCR) assay was successfully developed to simultaneously detect canine parainfluenza virus 5 (CPIV5) and a canine endogenous internal positive control (EIPC) in canine ...
Gyu-Tae Jeon +6 more
doaj +1 more source
Clinical Value of S-poly (T) plus Real-time PCR in Detection of Liver Cancer-related miR-199
Zhongliu Fangzhi Yanjiu, 2020 Objective To explore the advantages and disadvantages of S-poly(T) plus qRT-PCR and Poly (A) qRT-PCR methods in detecting peripheral blood serum miR-199.
WU Juan +5 more
doaj +1 more source
Spit or Swab? The Diagnostic Accuracy of Saliva-Based Testing as a Sars-Cov-2 Surveillance Tool
Philippine Journal of Pathology, 2022 Background. Nasopharyngeal swab/oropharyngeal swab (NPS/OPS) qRT-PCR is the gold standard for detecting SARS-CoV-2. However, it has its own limitations including cost and invasiveness.
Raymundo Lo +19 more
doaj +1 more source
Keeping qRT-PCR rigorous and biologically relevant [PDF]
Plant Cell Reports, 2014 Because of the importance of differential gene expression in the growth, development, and reproduction of organisms, assays of mRNA transcript levels are widely employed in biological research. The most frequently used assay combines reverse transcription (RT) with quantitative polymerase chain reaction (qPCR) and is known as qRT-PCR (or RT-qPCR).
John, Bennett +2 more
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qRT-PCR: a method and its difficulties [PDF]
Naunyn-Schmiedeberg's Archives of Pharmacology, 2012 Today, quantitative reverse transcription polymerase chain reaction (qRT-PCR) is the favored method to quantify gene expression in molecular biology and clinical studies. qRTPCR analyses can be performed in a high-throughput manner and are very sensitive and specific, cost-effective, and reproducible.
Franziska, Bollmann +3 more
openaire +2 more sources
Presence of SARS-CoV-2 in human tears detected by quantitative real time PCR (qRT-PCR)
Medical Journal of Dr. D.Y. Patil Vidyapeeth, 2022 Background: COVID-19 has caused a pandemic since the end of the year 2019. Controversy regarding the presence of the SARS-CoV-2 in tears and conjunctival sac has created suspense throughout.
Neeta Mishra +6 more
doaj +1 more source
Development of a Crystal Digital RT-PCR for the Detection of Atypical Porcine Pestivirus
Veterinary Sciences, 2023 Atypical porcine pestivirus (APPV), a newly discovered virus, is associated with the type A-II congenital tremor (CT) in neonatal piglets. APPV distributes throughout the world and causes certain economic losses to the swine industry.
Huixin Liu +5 more
doaj +1 more source
A two-step lyssavirus real-time polymerase chain reaction using degenerate primers with superior sensitivity to the fluorescent antigen test [PDF]
, 2014 A generic two-step lyssavirus real-time reverse transcriptase polymerase chain reaction (qRT-PCR), based on a nested PCR strategy, was validated for the detection of different lyssavirus species.
De Craeye, Stéphane +6 more
core +4 more sources
Virology Journal, 2020 Background Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2, is posing a serious threat to global public health. Reverse transcriptase real-time quantitative polymerase chain reaction (qRT-PCR) is widely used as the gold standard for clinical ...
Yang Zhang +10 more
doaj +1 more source
Development of reverse-transcription PCR techniques to analyse the density and sex ratio of gametocytes in genetically diverse Plasmodium chabaudi infections [PDF]
, 2007 We have developed cross-genotype and genotype-specific quantitative reverse-transcription PCR (qRT-PCR) assays to detect and quantify the number of parasites, transmission stages (gametocytes) and male gametocytes in blood stage Plasmodium chabaudi ...
Abdel-Wahab +44 more
core +1 more source