Results 11 to 20 of about 1,161,528 (204)
No control genes required: Bayesian analysis of qRT-PCR data.
PLoS ONE, 2013 BackgroundModel-based analysis of data from quantitative reverse-transcription PCR (qRT-PCR) is potentially more powerful and versatile than traditional methods.
Mikhail V Matz +2 more
doaj +5 more sources
Development of a qRT - PCR Diagnostic Kit for the Detection of FCoV
Cyprus Journal of Medical SciencesBACKGROUND/AIMS: In recent years, feline infectious peritonitis (FIP), which led to the death of more than 3,000 cats on the island of Cyprus, has become a significant concern. FIP is a fatal disease in cats caused by feline coronavirus (FCoV).
Gülten Tuncel Dereboylu +4 more
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qRT-PCR: a method and its difficulties [PDF]
Naunyn-Schmiedeberg's Archives of Pharmacology, 2012 Today, quantitative reverse transcription polymerase chain reaction (qRT-PCR) is the favored method to quantify gene expression in molecular biology and clinical studies. qRTPCR analyses can be performed in a high-throughput manner and are very sensitive and specific, cost-effective, and reproducible.
Franziska, Bollmann +3 more
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Keeping qRT-PCR rigorous and biologically relevant [PDF]
Plant Cell Reports, 2014 Because of the importance of differential gene expression in the growth, development, and reproduction of organisms, assays of mRNA transcript levels are widely employed in biological research. The most frequently used assay combines reverse transcription (RT) with quantitative polymerase chain reaction (qPCR) and is known as qRT-PCR (or RT-qPCR).
John, Bennett +2 more
openaire +2 more sources
Virology Journal, 2020 Background Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2, is posing a serious threat to global public health. Reverse transcriptase real-time quantitative polymerase chain reaction (qRT-PCR) is widely used as the gold standard for clinical ...
Yang Zhang +10 more
doaj +1 more source
Development of a Crystal Digital RT-PCR for the Detection of Atypical Porcine Pestivirus
Veterinary Sciences, 2023 Atypical porcine pestivirus (APPV), a newly discovered virus, is associated with the type A-II congenital tremor (CT) in neonatal piglets. APPV distributes throughout the world and causes certain economic losses to the swine industry.
Huixin Liu +5 more
doaj +1 more source