Results 21 to 30 of about 319,510 (207)

Statistical modeling for selecting housekeeper genes [PDF]

Genome Biology, 2004
Statistical models are presented for selecting the best housekeepers to normalize quantitative data within a given tissue type and across different types of tissue samples.There is a need for statistical methods to identify genes that have minimal variation in expression across a variety of experimental conditions.
Aniko, Szabo, Charles M, Perou, Mehmet, Karaca, Laurent, Perreard, Robert, Palais, John F, Quackenbush, Philip S, Bernard   +6 more
openaire   +2 more sources

Validation of suitable internal control genes for expression studies in aging. [PDF]

, 2009
Quantitative data from experiments of gene expression are often normalized through levels of housekeeping genes transcription by assuming that expression of these genes is highly uniform. This practice is being questioned as it becomes increasingly clear
Bacalini, MARIA GIULIA, Burkle, A, Caiafa, Paola, Calabrese, Roberta, Chevanne, M, Ciccarone, Fabio, Guastafierro, Tiziana, MORENO VILLANUEVA, M, Reale, Anna, Zampieri, Michele   +9 more
core   +1 more source

Gene expression in chicken reveals correlation with structural genomic features and conserved patterns of transcription in the terrestrial vertebrates. [PDF]

PLoS ONE, 2010
BACKGROUND: The chicken is an important agricultural and avian-model species. A survey of gene expression in a range of different tissues will provide a benchmark for understanding expression levels under normal physiological conditions in birds.
Haisheng Nie, Richard P M A Crooijmans, Aart Lammers, Evert M van Schothorst, Jaap Keijer, Pieter B T Neerincx, Jack A M Leunissen, Hendrik-Jan Megens, Martien A M Groenen   +8 more
doaj   +1 more source

Soft topographic map for clustering and classification of bacteria [PDF]

, 2007
In this work a new method for clustering and building a topographic representation of a bacteria taxonomy is presented. The method is based on the analysis of stable parts of the genome, the so-called “housekeeping genes”.
G.M. Garrity, H. Klock, I.T. Joliffe, J.D. Thompson, J.E. Clarridge III, K. Rose, M. Drancourt, M. Drancourt, M. Drancourt, M. Remm, P. Rice, S. Altschul, S. Dubnov, S. Kumar, S.B. Needleman, S.P. Luttrell, T. Graepel, T. Graepel, T. Hofmann, T. Hofmann, T. Kohonen, T. Kohonen, T.H. Jukes, W.S. Torgerson   +23 more
core   +2 more sources

GAPDH as housekeeping gene at renal level [PDF]

Kidney International, 2004
To the Editor: In mRNA quantitative studies, choosing a reliable internal control is mandatory for monitoring intersample variations and comparing target RNA levels if assays are performed in different experimental sessions and come from different centers.
CEOL, MONICA, DEL PRETE, DORELLA, TOSETTO, ENRICA, GRAZIOTTO, ROMINA, GAMBARO G, D'ANGELO, ANGELA, ANGLANI, FRANCA   +6 more
openaire   +3 more sources

Average nucleotide identity of genome sequences supports the description of Rhizobium lentis sp. nov., Rhizobium bangladeshense sp. nov. and Rhizobium binae sp. nov. from lentil (Lens culinaris) nodules [PDF]

, 2015
Rhizobial strains isolated from effective root nodules of field-grown lentil (Lens culinaris) from different parts of Bangladesh were previously analysed using sequences of the 16S rRNA gene, three housekeeping genes (recA, atpD and glnll) and three ...
Braun, Markus Santosh, Clercx, Pia, Everall, Isobel, Rashid, M Harun-Or, Willems, Anne, Wink, Michael, Young, J Peter W   +6 more
core   +2 more sources

The replacement histone H2A.Z in a hyperacetylated form is a feature of active genes in the chicken [PDF]

, 2005
The replacement histone H2A.Z is variously reported as being linked to gene expression and preventing the spread of heterochromatin in yeast, or concentrated at heterochromatin in mammals.
Bonifer, C., Bruce, K., Crane-Robinson, C., Greaves, I., Lefevre, P., Mantouvalou, E., Myers, F.A., Thorne, A.W., Tremethick, D.J.   +8 more
core   +5 more sources

Identification of candidate reference genes for qRT-PCR normalization studies of salinity stress and injury in Onchidium reevesii [PDF]

PeerJ, 2019
Real-time quantitative reverse transcription-PCR (qRT-PCR) is an undeniably effective tool for measuring levels of gene expression, but the accuracy and reliability of the statistical data obtained depend mainly on the basal expression of selected ...
Teizhu Yang, Bingning Gu, Guolyu Xu, Yanmei Shi, Heding Shen, Rongcheng Rao, Hellen Lucas Mzuka   +6 more
doaj   +2 more sources

An expanded multilocus sequence typing scheme for propionibacterium acnes : investigation of 'pathogenic', 'commensal' and antibiotic resistant strains [PDF]

, 2012
The Gram-positive bacterium Propionibacterium acnes is a member of the normal human skin microbiota and is associated with various infections and clinical conditions.
Barnard, E. (Emma), Cove, Jonathan, Eady, Anne, Gao, Anna, Li, Huiying, McDowell, A. (Andrew), Nagy, I. (István), Nord, Carl Erik, Patrick, Sheila, Tomida, Shuta   +9 more
core   +3 more sources

Do Housekeeping Genes Exist?

PLOS ONE, 2015
The searching of human housekeeping (HK) genes has been a long quest since the emergence of transcriptomics, and is instrumental for us to understand the structure of genome and the fundamentals of biological processes. The resolved genes are frequently used in evolution studies and as normalization standards in quantitative gene-expression analysis ...
Zhang, Yijuan, Li, Ding, Sun, Bingyun
openaire   +5 more sources