Results 31 to 40 of about 319,510 (207)
The aim of this study was designed to select housekeeping genes for quantitative gene expression analysis in yellow-feathered broilers. Twelve 3-week-old chickens were randomly selected from 60 yellow-feathered broilers.
Jie Zhang +5 more
doaj +1 more source
Multiple gene sequence analysis using genes of the bacterial DNA repair pathway
The ability to recognize and repair abnormal DNA structures is common to all forms of life. Physiological studies and genomic sequencing of a variety of bacterial species have identified an incredible diversity of DNA repair pathways.
Miguel Rotelok Neto +6 more
doaj +1 more source
There are no validated housekeeping genes in induced pluripotent stem cells (iPSC) and derived endothelial iPSC (iPSC-EC). Thus a comparison of gene expression levels is less reliable, especially during drug treatments.
Sheena L. M. Ong +4 more
doaj +1 more source
High-Throughput and Cost-Effective Characterization of Induced Pluripotent Stem Cells. [PDF]
Reprogramming somatic cells to induced pluripotent stem cells (iPSCs) offers the possibility of studying the molecular mechanisms underlying human diseases in cell types difficult to extract from living patients, such as neurons and cardiomyocytes.
Arias, Angelo +12 more
core +2 more sources
Identification of Suitable Meloidogyne spp. Housekeeping Genes [PDF]
Abstract Gene expression studies often require reliable housekeeping (HK) genes to accurately capture gene expression levels under given conditions. This is especially true for root-knot nematodes (RKN, Meloidogyne spp.), whose drastic developmental changes are strongly dependent upon their environment.
Hu, Weiming, DiGennaro, Peter M.
openaire +3 more sources
The choice of an appropriate housekeeping gene for normalisation purposes has now become an essential requirement when designing QPCR experiments. This is of particular importance when using QPCR to measure viral and cellular gene transcription levels in
Wilkinson John +5 more
doaj +1 more source
Evaluation of potential reference genes for real-time qPCR analysis in a biparental beetle, Lethrus apterus (Coleoptera: Geotrupidae) [PDF]
Hormones play an important role in the regulation of physiological, developmental and behavioural processes. Many of these mechanisms in insects, however, are still not well understood.
Nikoletta A. Nagy +6 more
doaj +2 more sources
Multilocus sequence analysis of xanthomonads causing bacterial spot of tomato and pepper plants reveals strains generated by recombination among species and recent global spread of Xanthomonas gardneri [PDF]
Four Xanthomonas species are known to cause bacterial spot of tomato and pepper, but the global distribution and genetic diversity of these species are not well understood.
Bart, Rebecca +12 more
core +1 more source
Housekeeping genes as internal standards: use and limits [PDF]
Quantitative studies are commonly realised in the biomedical research to compare RNA expression in different experimental or clinical conditions. These quantifications are performed through their comparison to the expression of the housekeeping gene transcripts like glyceraldehyde-3-phosphate dehydrogenase (G3PDH), albumin, actins, tubulins ...
Thellin, Olivier +8 more
openaire +2 more sources
Results of overexpression or downregulation of a microRNA (miRNA) on its target mRNA expression are often validated by reverse-transcription and quantitative PCR analysis using an appropriate housekeeping gene as an internal control.
Kavleen Sikand +3 more
doaj +1 more source

