Results 31 to 40 of about 319,510 (207)

Selection of housekeeping genes for quantitative gene expression analysis in yellow-feathered broilers

open access: yesItalian Journal of Animal Science, 2018
The aim of this study was designed to select housekeeping genes for quantitative gene expression analysis in yellow-feathered broilers. Twelve 3-week-old chickens were randomly selected from 60 yellow-feathered broilers.
Jie Zhang   +5 more
doaj   +1 more source

Multiple gene sequence analysis using genes of the bacterial DNA repair pathway

open access: yesBrazilian Archives of Biology and Technology, 2015
The ability to recognize and repair abnormal DNA structures is common to all forms of life. Physiological studies and genomic sequencing of a variety of bacterial species have identified an incredible diversity of DNA repair pathways.
Miguel Rotelok Neto   +6 more
doaj   +1 more source

Identification of stable housekeeping genes for induced pluripotent stem cells and -derived endothelial cells for drug testing

open access: yesScientific Reports, 2022
There are no validated housekeeping genes in induced pluripotent stem cells (iPSC) and derived endothelial iPSC (iPSC-EC). Thus a comparison of gene expression levels is less reliable, especially during drug treatments.
Sheena L. M. Ong   +4 more
doaj   +1 more source

High-Throughput and Cost-Effective Characterization of Induced Pluripotent Stem Cells. [PDF]

open access: yes, 2017
Reprogramming somatic cells to induced pluripotent stem cells (iPSCs) offers the possibility of studying the molecular mechanisms underlying human diseases in cell types difficult to extract from living patients, such as neurons and cardiomyocytes.
Arias, Angelo   +12 more
core   +2 more sources

Identification of Suitable Meloidogyne spp. Housekeeping Genes [PDF]

open access: yesJournal of Nematology, 2019
Abstract Gene expression studies often require reliable housekeeping (HK) genes to accurately capture gene expression levels under given conditions. This is especially true for root-knot nematodes (RKN, Meloidogyne spp.), whose drastic developmental changes are strongly dependent upon their environment.
Hu, Weiming, DiGennaro, Peter M.
openaire   +3 more sources

Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses

open access: yesVirology Journal, 2007
The choice of an appropriate housekeeping gene for normalisation purposes has now become an essential requirement when designing QPCR experiments. This is of particular importance when using QPCR to measure viral and cellular gene transcription levels in
Wilkinson John   +5 more
doaj   +1 more source

Evaluation of potential reference genes for real-time qPCR analysis in a biparental beetle, Lethrus apterus (Coleoptera: Geotrupidae) [PDF]

open access: yesPeerJ, 2017
Hormones play an important role in the regulation of physiological, developmental and behavioural processes. Many of these mechanisms in insects, however, are still not well understood.
Nikoletta A. Nagy   +6 more
doaj   +2 more sources

Multilocus sequence analysis of xanthomonads causing bacterial spot of tomato and pepper plants reveals strains generated by recombination among species and recent global spread of Xanthomonas gardneri [PDF]

open access: yes, 2015
Four Xanthomonas species are known to cause bacterial spot of tomato and pepper, but the global distribution and genetic diversity of these species are not well understood.
Bart, Rebecca   +12 more
core   +1 more source

Housekeeping genes as internal standards: use and limits [PDF]

open access: yesJournal of Biotechnology, 1999
Quantitative studies are commonly realised in the biomedical research to compare RNA expression in different experimental or clinical conditions. These quantifications are performed through their comparison to the expression of the housekeeping gene transcripts like glyceraldehyde-3-phosphate dehydrogenase (G3PDH), albumin, actins, tubulins ...
Thellin, Olivier   +8 more
openaire   +2 more sources

Housekeeping gene selection advisory: glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-actin are targets of miR-644a.

open access: yesPLoS ONE, 2012
Results of overexpression or downregulation of a microRNA (miRNA) on its target mRNA expression are often validated by reverse-transcription and quantitative PCR analysis using an appropriate housekeeping gene as an internal control.
Kavleen Sikand   +3 more
doaj   +1 more source

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