An MLSA-based online scheme for the rapid identification of Stenotrophomonas isolates [PDF]
An online scheme to assign Stenotrophomonas isolates to genomic groups was developed using the multilocus sequence analysis (MLSA), which is based on the DNA sequencing of selected fragments of the housekeeping genes ATP synthase alpha subunit (atpA ...
Barbosa, Heloiza Ramos +8 more
core +3 more sources
Gene expression studies employing real-time PCR has become an intrinsic part of biomedical research. Appropriate normalization of target gene transcript(s) based on stably expressed housekeeping genes is crucial in individual experimental conditions to ...
Deepali eMathur +6 more
doaj +1 more source
Evolutionary dynamics of insertion sequences in relation to the evolutionary histories of the chromosome and symbiotic plasmid genes of Rhizobium etli populations [PDF]
Insertion sequences (IS) are mobile genetic elements that are distributed in many prokaryotes. In particular, in the genomes of the symbiotic nitrogen-fixing bacteria collectively known as rhizobia, IS are fairly abundant in plasmids or chromosomal ...
Bustos, Patricia +7 more
core +2 more sources
Accelerated Evolutionary Rate of Housekeeping Genes in Tunicates [PDF]
Phylogenomics has recently revealed that tunicates represent the sister-group of vertebrates in the newly defined clade Olfactores. However, phylogenomic and comparative genomic studies have also suggested that tunicates are characterized by an elevated rate of molecular evolution and a high degree of genomic divergence.
Tsagkogeorga, Georgia +4 more
openaire +5 more sources
Detection bias in microarray and sequencing transcriptomic analysis identified by housekeeping genes
This work includes the original data used to discover the gene ontology bias in transcriptomic analysis conducted by microarray and high throughput sequencing (Zhang et al., 2015) [1].
Yijuan Zhang +3 more
doaj +1 more source
Identification of a novel human E-Cadherin splice variant andassessment of its effects upon EMT-related events [PDF]
Epithelial Cadherin (E-cadherin) is involved in calcium-dependent cell-cell adhesion and signal transduction. The E-cadherin decrease/loss is a hallmark of Epithelial to Mesenchymal Transition (EMT), a key event in tumor progression.
Besso, María José +8 more
core +1 more source
Use of whole-genus genome sequence data to develop a multilocus sequence typing tool that accurately identifies Yersinia isolates to the species and subspecies levels [PDF]
The genus Yersinia is a large and diverse bacterial genus consisting of human-pathogenic species, a fish-pathogenic species, and a large number of environmental species.
Carniel, Elisabeth +13 more
core +1 more source
Mammalian Housekeeping Genes Evolve More Slowly than Tissue-Specific Genes [PDF]
Do housekeeping genes, which are turned on most of the time in almost every tissue, evolve more slowly than genes that are turned on only at specific developmental times or tissues? Recent large-scale gene expression studies enable us to have a better definition of housekeeping genes and to address the above question in detail.
Liqing, Zhang, Wen-Hsiung, Li
openaire +2 more sources
Choroid plexus (ChP), a vascularized secretory epithelium located in all brain ventricles, plays critical roles in development, homeostasis and brain repair. Reverse transcription quantitative real-time PCR (RT-qPCR) is a popular and useful technique for
Kim Hoa Ho, Annarita Patrizi
doaj +1 more source
Complete genome sequencing of mycoplasma bovis type strain ningxia-1 and systematic bioinformatic characterization for housekeeping-related genes [PDF]
Mycoplasma bovis is a major pathogen leading to bovine respiratory disease syndrome. The worldwide prevalence of this pathogen has caused enormous economic losses in the beef industry.
Peng SUN +5 more
doaj +1 more source

