Results 31 to 40 of about 174,210 (250)

Repetitive sequence environment distinguishes housekeeping genes [PDF]

Gene, 2007
Housekeeping genes are expressed across a wide variety of tissues. Since repetitive sequences have been reported to influence the expression of individual genes, we employed a novel approach to determine whether housekeeping genes can be distinguished from tissue-specific genes by their repetitive sequence context.
C Daniel, Eller, Moira, Regelson, Barry, Merriman, Stan, Nelson, Steve, Horvath, York, Marahrens   +5 more
openaire   +2 more sources

Statistical modeling for selecting housekeeper genes [PDF]

Genome Biology, 2004
Statistical models are presented for selecting the best housekeepers to normalize quantitative data within a given tissue type and across different types of tissue samples.There is a need for statistical methods to identify genes that have minimal variation in expression across a variety of experimental conditions.
Aniko, Szabo, Charles M, Perou, Mehmet, Karaca, Laurent, Perreard, Robert, Palais, John F, Quackenbush, Philip S, Bernard   +6 more
openaire   +2 more sources

Using ribosomal protein genes as reference: a tale of caution. [PDF]

PLoS ONE, 2008
BACKGROUND: Housekeeping genes are needed in every tissue as their expression is required for survival, integrity or duplication of every cell. Housekeeping genes commonly have been used as reference genes to normalize gene expression data, the ...
Lieven Thorrez, Katrijn Van Deun, Léon-Charles Tranchevent, Leentje Van Lommel, Kristof Engelen, Kathleen Marchal, Yves Moreau, Iven Van Mechelen, Frans Schuit   +8 more
doaj   +1 more source

GAPDH as housekeeping gene at renal level [PDF]

Kidney International, 2004
To the Editor: In mRNA quantitative studies, choosing a reliable internal control is mandatory for monitoring intersample variations and comparing target RNA levels if assays are performed in different experimental sessions and come from different centers.
CEOL, MONICA, DEL PRETE, DORELLA, TOSETTO, ENRICA, GRAZIOTTO, ROMINA, GAMBARO G, D'ANGELO, ANGELA, ANGLANI, FRANCA   +6 more
openaire   +3 more sources

Isolation of Housekeeping Genes on Durik-durik (Syzygium sp)

Biosaintifika: Journal of Biology & Biology Education, 2018
Housekeeping gene is a gene expressed with a fixed level and in abundant amounts under various conditions. After validation, the housekeeping gene can be used as an internal control to normalize gene expression data.
Dewi Indriyani Roslim, Liza Aulia Yusfi, Desriani Ritawati Hutagalung, Niswah Faizah, Lestari Andriani, Azrial Azrial, Herman Herman, Wahyu Lestari   +7 more
doaj   +1 more source

Genetic and symbiotic characterization of rhizobia nodulating legumes in a mining area in southeast Brazil

Scientia Agricola, 2021
: Identification and selection of nitrogen-fixing bacterial strains for inoculation into native leguminous tree species can assist in the recovery of degraded areas.
Bruna Daniela Ortiz Lopez, Anita Fernanda dos Santos Teixeira, Daniele Cabral Michel, Amanda Azarias Guimarães, Amanda Monique da Costa, Juliana Santos Costa, Marcela de Souza Pereira, Bárbara Luyse Marques Duarte, Fatima Maria de Souza Moreira   +8 more
doaj   +1 more source

Validation of housekeeping genes for normalizing RNA expression in real-time PCR

BioTechniques, 2004
Analysis of RNA expression using techniques like real-time PCR has traditionally used reference or housekeeping genes to control for error between samples.
Keertan Dheda, Jim F. Huggett, Stephen A. Bustin, Margaret A. Johnson, Graham Rook, Alimuddin Zumla   +5 more
doaj   +1 more source

Multiple gene sequence analysis using genes of the bacterial DNA repair pathway

Brazilian Archives of Biology and Technology, 2015
The ability to recognize and repair abnormal DNA structures is common to all forms of life. Physiological studies and genomic sequencing of a variety of bacterial species have identified an incredible diversity of DNA repair pathways.
Miguel Rotelok Neto, Carolina Weigert Galvão, Leonardo Magalhães Cruz, Dieval Guizelini, Leilane Caline Silva, Jarem Raul Garcia, Rafael Mazer Etto   +6 more
doaj   +1 more source

Complete genome sequencing of mycoplasma bovis type strain ningxia-1 and systematic bioinformatic characterization for housekeeping-related genes [PDF]

Kafkas Universitesi Veteriner Fakültesi Dergisi, 2020
Mycoplasma bovis is a major pathogen leading to bovine respiratory disease syndrome. The worldwide prevalence of this pathogen has caused enormous economic losses in the beef industry.
Peng SUN, Yong FU, Qiaofeng WAN, Mohamed YOSRI, Shenghu HE, Xiuying SHEN   +5 more
doaj   +1 more source

Selection and Validation of Potential Reference Genes for Quantitative Real-Time PCR Analysis in Blaptica Dubia (Serville, 1838) (Blattidae, Blaberidae)

Cumhuriyet Science Journal, 2022
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is an effective, reproducible, and dependable method for evaluating and targeting expression of genes.
Emin Ufuk Karakaş, Şeyda Berk, Ayşe Nur Pektaş   +2 more
doaj   +1 more source