Results 41 to 50 of about 174,210 (250)

A comprehensive functional analysis of tissue specificity of human gene expression

BMC Biology, 2008
Background In recent years, the maturation of microarray technology has allowed the genome-wide analysis of gene expression patterns to identify tissue-specific and ubiquitously expressed ('housekeeping') genes.
Guryanov Alexey, Brennan Richard J, Rakhmatulin Eugene, Bugrim Andrej, Dosymbekov Damir, Serebriyskaya Tatiana, Shi Weiwei, Sviridov Evgeny, Nikolsky Yuri, Dezső Zoltán, Li Kelly, Blake Julie, Samaha Raymond R, Nikolskaya Tatiana   +13 more
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Identification and validation of suitable housekeeping genes for normalizing quantitative real-time PCR assays in injured peripheral nerves. [PDF]

PLoS ONE, 2014
Injury to the peripheral nerve induces dramatic changes in terms of cellular composition that are reflected on RNA quality and quantity, making messenger RNA expression analysis very complex.
Giovanna Gambarotta, Giulia Ronchi, Olivier Friard, Pantaleo Galletta, Isabelle Perroteau, Stefano Geuna   +5 more
doaj   +1 more source

Housekeeping genes as internal standards: use and limits [PDF]

Journal of Biotechnology, 1999
Quantitative studies are commonly realised in the biomedical research to compare RNA expression in different experimental or clinical conditions. These quantifications are performed through their comparison to the expression of the housekeeping gene transcripts like glyceraldehyde-3-phosphate dehydrogenase (G3PDH), albumin, actins, tubulins ...
Thellin, Olivier, Zorzi, Willy, Lakaye, Bernard, De Borman, B., Coumans, Bernard, Hennen, Georges, Grisar, Thierry, Igout, Ahmed, Heinen, Ernst   +8 more
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Identification of human housekeeping genes and tissue-selective genes by microarray meta-analysis. [PDF]

PLoS ONE, 2011
BACKGROUND: Categorizing protein-encoding transcriptomes of normal tissues into housekeeping genes and tissue-selective genes is a fundamental step toward studies of genetic functions and genetic associations to tissue-specific diseases. Previous studies
Cheng-Wei Chang, Wei-Chung Cheng, Chaang-Ray Chen, Wun-Yi Shu, Min-Lung Tsai, Ching-Lung Huang, Ian C Hsu   +6 more
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Accelerated Evolutionary Rate of Housekeeping Genes in Tunicates [PDF]

Journal of Molecular Evolution, 2010
Phylogenomics has recently revealed that tunicates represent the sister-group of vertebrates in the newly defined clade Olfactores. However, phylogenomic and comparative genomic studies have also suggested that tunicates are characterized by an elevated rate of molecular evolution and a high degree of genomic divergence.
Tsagkogeorga, Georgia, Turon, Xavier, Galtier, Nicolas, Douzery, Emmanuel, Delsuc, Frédéric   +4 more
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Gene expression in chicken reveals correlation with structural genomic features and conserved patterns of transcription in the terrestrial vertebrates. [PDF]

PLoS ONE, 2010
BACKGROUND: The chicken is an important agricultural and avian-model species. A survey of gene expression in a range of different tissues will provide a benchmark for understanding expression levels under normal physiological conditions in birds.
Haisheng Nie, Richard P M A Crooijmans, Aart Lammers, Evert M van Schothorst, Jaap Keijer, Pieter B T Neerincx, Jack A M Leunissen, Hendrik-Jan Megens, Martien A M Groenen   +8 more
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Mammalian Housekeeping Genes Evolve More Slowly than Tissue-Specific Genes [PDF]

Molecular Biology and Evolution, 2004
Do housekeeping genes, which are turned on most of the time in almost every tissue, evolve more slowly than genes that are turned on only at specific developmental times or tissues? Recent large-scale gene expression studies enable us to have a better definition of housekeeping genes and to address the above question in detail.
Liqing, Zhang, Wen-Hsiung, Li
openaire   +2 more sources

Genetic Diversity of Neisseria gonorrhoeae Housekeeping Genes [PDF]

Journal of Clinical Microbiology, 2003
ABSTRACT Molecular typing of Neisseria gonorrhoeae strains is an important tool for epidemiological studies of gonococcal infection and transmission. The recently developed multilocus sequence typing (MLST) method is based on the genetic variation among housekeeping genes.
Raphael P, Viscidi, James C, Demma
openaire   +2 more sources

Housekeeping gene validation for RT-qPCR studies on synovial fibroblasts derived from healthy and osteoarthritic patients with focus on mechanical loading.

PLoS ONE, 2019
Selection of appropriate housekeeping genes is essential for the validity of data normalization in reverse transcription quantitative PCR (RT-qPCR). Synovial fibroblasts (SF) play a mediating role in the development and progression of osteoarthritis (OA)
Ute Nazet, Agnes Schröder, Susanne Grässel, Dominique Muschter, Peter Proff, Christian Kirschneck   +5 more
doaj   +1 more source

Selection of housekeeping genes for quantitative gene expression analysis in yellow-feathered broilers

Italian Journal of Animal Science, 2018
The aim of this study was designed to select housekeeping genes for quantitative gene expression analysis in yellow-feathered broilers. Twelve 3-week-old chickens were randomly selected from 60 yellow-feathered broilers.
Jie Zhang, Yu-Yun Gao, Yi-Qiang Huang, Qian Fan, Xin-Tao Lu, Chang-Kang Wang   +5 more
doaj   +1 more source