Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
Author Correction: Chemokine-defined macrophage niches establish spatial organization of tumor immunity. [PDF]
Ghosh S +8 more
europepmc +1 more source
Author Correction: Droughts preceding tree mortality events have increased in duration and intensity, especially in dry biomes. [PDF]
Gazol A +4 more
europepmc +1 more source
Aging impact on canine extracellular vesicles production, size, and miRNA content. [PDF]
Marina CL +11 more
europepmc +1 more source
Nicotinamide riboside reduces glial inflammation and boosts mitochondrial function. [PDF]
Yangzom T +3 more
europepmc +1 more source
Integrative Bioinformatics Analysis Reveals COL13A1 and COL23A1 as Potential Diagnostic and Prognostic Biomarkers in Thyroid Cancer. [PDF]
Islam MW +6 more
europepmc +1 more source
Cardiomyopathy and mitochondrial encephalomyopathy in a female child associated with a heterozygous X-linked AIFM1 variant. [PDF]
Sandmann C +5 more
europepmc +1 more source

