Results 81 to 90 of about 56,323 (263)

Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region

FEBS Open Bio, EarlyView.
Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson, Anja H. Schiemann, Evelyn Sattlegger   +2 more
wiley   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Optimisation of Garbage Bin Layout in Rural Infrastructure for Promoting the Renovation of Rural Human Settlements: Case Study of Yuding Village in China. [PDF]

Int J Environ Res Public Health, 2021
Shen Y, Wang C.
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

The role of agricultural projects interventions on the incomes and material wealth among farming youth in Tanzania. A case study of Kilombero District

Cogent Food & Agriculture
Agricultural development projects play a crucial role in enhancing the livelihoods of young farmers in rural areas. This study investigated the impact of such project interventions on income levels and material wealth among youth engaged in farming in ...
Deogratias Clemence Maksi, Makarius Victor Mdemu, Tatu Mtwangi Limbumba   +2 more
doaj   +1 more source

Puma (Puma concolor) in the Neighborhood? Records Near Human Settlements and Insights into Human-Carnivore Coexistence in Central Chile. [PDF]

Animals (Basel), 2021
Ramírez-Álvarez D, Napolitano C, Salgado I.   +2 more
europepmc   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

The Impact of System Softness on Haines Jumps and Drainage in Porous Media

Water Resources Research
Haines jumps are abrupt movements of fluid interfaces accompanied by sudden changes in capillary pressure during multiphase flow in porous media. They are a fundamental pore‐scale mechanism influencing displacement patterns and efficiency.
Bowen Zhang, Dianrun Yang, Ling Xu, Zhonghao Sun   +3 more
doaj   +1 more source

High-Rise Residential District Morphology Optimization for Enhancing the Green Space Cooling Effect

Buildings
Large-scale urban green spaces exert a cooling effect in cities and have great potential for optimizing the urban climate. In this study, taking the typical green space Xingfulindai in Xi’an as an example, we carried out field measurements and ENVI-met ...
Feng Shi, Yuan Chen, Wenru Yue, Yupeng Wang   +3 more
doaj   +1 more source

Chronobiology of Cancer: How Aging Fuels Oncogenesis at the Molecular Level

Aging and Cancer, EarlyView.
This graphical abstract illustrates the key biological pathways linking aging with cancer development and progression. In the upper left, cumulative exposure to ultraviolet radiation, toxins, and reactive oxygen species (ROS) causes DNA damage and genomic instability, whereas age‐related decline in repair mechanisms, such as ATM/ATR, BER, and NER ...
Anu Singh, Aroonima Misra, Sufian Zaheer
wiley   +1 more source