Results 81 to 90 of about 28,002,475 (294)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Investigation of mixed element hybrid grid-based CFD methods for rotorcraft flow analysis [PDF]

, 2010
Accurate first-principles flow prediction is essential to the design and development of rotorcraft, and while current numerical analysis tools can, in theory, model the complete flow field, in practice the accuracy of these tools is limited by various ...
Smith, Marlyn J., Whitehouse, Glen R., Boschitsch, Alexander H., Lynch, C. Eric, Brown, Richard   +4 more
core   +3 more sources

A Study on Adaptive Implicit–Explicit and Explicit–Explicit Time Integration Procedures for Wave Propagation Analyses

Acoustics
This study delves into the effectiveness of two time integration techniques, namely the adaptive implicit–explicit (imp–exp) and explicit–explicit (exp–exp) methods, which stand as efficient formulations for tackling intricate systems characterized by ...
Delfim Soares, Isabelle de Souza Sales, Lucas Ruffo Pinto, Webe João Mansur   +3 more
doaj   +1 more source

From patient advocacy to patient‐driven research: Building active partnerships beginning at the bench to reach the bedside

FEBS Open Bio, EarlyView.
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia, Rachel Heilmann, Maximiliano Barrientos, Laura MacNeill, Ingrid Vallee, Sarah K. Schultz, Patrick O'Donoghue, Angelica A. Moresco, Victoria M. Siu, Ilka U. Heinemann   +9 more
wiley   +1 more source

The Analysis of Hybrid Trie Structures.

, 1997
This paper provides a detailed analysis of various implementations of digital tries, including the "ternary search tries" of Bentley and Sedgewick. The methods employed combine symbolic uses of generating functions, Poisson models, and Mellin transforms.
Clément, Julien, Flajolet, Philippe, Vallée, Brigitte   +2 more
openaire   +3 more sources

Hybrid System for solving MPC of piecewise affine system

, 2003
IFAC Analysis and Design of Hybrid Systems, Saint-Malo-Brittany - Francia, 16/06/2003This paper presents a hybrid procedure to solve Model Predictive Control (MPC) of Piecewice Affine (PWA) system.
Camacho, Eduardo F., Miguel Peña, Peña, Miguel, Sandra Piñón, Piñón, Sandra, E.F. Camacho   +5 more
core   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Hybrid bases in graphs [PDF]

In this paper we introduce a new concept, that of hybrid base, which is a maximal circuitless and cutsetless subset of a graph. Although this concept of simultaneous circuitlessness and cutsetlessness has been used in proofs of some theorems in so called
Gibbons, Alan, Novak, Ladislav
core  

Using cell‐free RNA to identify B‐ and T‐cell clonality for diagnosis and monitoring of B‐ and T‐cell neoplasms

FEBS Open Bio, EarlyView.
Using peripheral blood for determining B‐cell or T‐cell clonality is more reliable when we use cell‐free RNA (cfRNA) because cells release blood significantly more RNA than DNA. Next‐generation sequencing (NGS) of cfRNA allows us to evaluate fragment cfRNA and evaluate clonality reliably without the need for prior determination of the specific dominant
Adam Albitar, Sally Agersborg, Ahmed Charifa, Pooja Phull, Noa Biran, David Vesole, Harsh Parmar, Andrew Pecora, Andrew Ip, Andre Goy, David Siegel, Maher Albitar   +11 more
wiley   +1 more source

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source