Results 61 to 70 of about 1,823,632 (294)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Vector Directional Distance Rational Hybrid Filters for Color Image Restoration

The Journal of Engineering Research, 2005
A new class of nonlinear filters, called vector-directional distance rational hybrid filters (VDDRHF) for multispectral image processing, is introduced and applied to color image-filtering problems. These filters are based on rational functions (RF). The
L. Khriji
doaj   +1 more source

Power quality improvement of sugar factories dc motor drive using hybrid filter [PDF]

Egyptian Sugar Journal, 2019
Power quality management is the main problem facing industry today. This problem is mainly due to the generation of electric network harmonics. The growing use of variable speed drives produces a large amount of harmonics in distribution systems because ...
Mohamed Nosier, Fareed Mohamed, Gaber Ahmed   +2 more
doaj   +1 more source

Hybrid Color Filters for Multispectral Imaging

Advanced Theory and Simulations, 2020
AbstractMultispectral cameras capture images in multiple wavelengths in narrow spectral bands. They offer advanced sensing well beyond normal cameras and many single‐sensor‐based multispectral cameras are commercialized aimed at a broad range of applications, such as agroforestry research, medical analysis, and so on.
Xin He, Yajing Liu, Paul Beckett, Md Hemayet Uddin, Ampalavanapillai Nirmalathas, Ranjith Rajasekharan Unnithan   +5 more
openaire   +2 more sources

Identification and characterization of a gene conferring stress resistance in Escherichia coli and cyanobacteria

FEBS Open Bio, EarlyView.
Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada, Takaaki Tanaka, Manaka Uehara, Keigo Nakano, Ken Morishima, Rintaro Inoue, Masaaki Sugiyama, Masafumi Yohda   +7 more
wiley   +1 more source

Accurate Computation of Vocal Tract Filter Parameters Using a Hybrid Genetic Algorithm

Applied Artificial Intelligence, 2019
This paper reports an original technique for accurately estimating the parameters of human vocal tract filters for vowels in English for speech processing applications such as voice recognition.
Mathew Mithra Noel, Venkataraman Muthiah-Nakarajan, Ruban Nersisson   +2 more
doaj   +1 more source

Hybrid Overlap Filter for LiDAR Point Clouds Using Free Software

Remote Sensing, 2020
Despite the large amounts of resources destined to developing filtering algorithms of LiDAR point clouds in order to obtain a Digital Terrain Model (DTM), the task remains a challenge. As a society advancing towards the democratization of information and
Sandra Buján, Miguel Cordero, David Miranda   +2 more
doaj   +1 more source

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells

FEBS Open Bio, EarlyView.
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley   +1 more source

Optimal Baseflow Separation Through Chemical Mass Balance: Comparing the Usages of Two Tracers, Two Concentration Estimation Methods, and Four Baseflow Filters

Water Resources Research
Optimizing empirical baseflow filters using environmental tracers (e.g., specific electrical conductance (SEC), turbidity) is an effective and efficient way to quantify the contribution of baseflow to total flow.
Yiwen Mei, Dagang Wang, Jinxin Zhu, Guoping Tang, Chenkai Cai, Xinyi Shen, Yi Hong, Xinxuan Zhang   +7 more
doaj   +1 more source