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RNA colony hybridization method
Gene, 1986A method for rapid screening of specific RNA sequences in recombinant colonies by hybridization in situ is presented. The method includes two consecutive steps of lytic treatment of the nitrocellulose-filter-supported colonies (10% sodium dodecyl sulfate and 3 X SSC at 65 degrees C) and hybridization with 32P-labelled specific oligodeoxynucleotides.
I, Ivanov, L, Gigova
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Journal of Chromatography A, 1986
Abstract A new method of identification and determination of nanogram of substances is proposed, which is a combination of separation by thin-layer chromatography and X-ray fluorescence microanalysis of the separated components in the zones on the chromatogram.
M.P. Volynets, V.G. Berezkin, N.P. Ilyin
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Abstract A new method of identification and determination of nanogram of substances is proposed, which is a combination of separation by thin-layer chromatography and X-ray fluorescence microanalysis of the separated components in the zones on the chromatogram.
M.P. Volynets, V.G. Berezkin, N.P. Ilyin
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