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Current Protocols in Molecular Biology, 1989
AbstractAutoradiography is used to detect and quantitate radioactive probe hybridized to cytological preparations. Autoradiographic film is used to detect 32P‐ or 35S‐labeled probe, and can be useful in experiments dealing with large organs or tissues. Emulsion autoradiography is required to obtain resolution at the level of a single cell.
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AbstractAutoradiography is used to detect and quantitate radioactive probe hybridized to cytological preparations. Autoradiographic film is used to detect 32P‐ or 35S‐labeled probe, and can be useful in experiments dealing with large organs or tissues. Emulsion autoradiography is required to obtain resolution at the level of a single cell.
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In situ hybridization with oligonucleotide probes
2002Publisher Summary In situ hybridization (ISH) is an important method for tracing the regional and cellular sites of gene expression (mRNA distribution) within a tissue. This method comes into its own when applied to the intermingled cell types of the brain.
W, Wisden, B J, Morris
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Amplifiable hybridization probes.
Annales de biologie clinique, 1990Amplifiable hybridization probes enable the development of extremely sensitive clinical assays. These novel molecules consist of a probe sequence embedded within the sequences of a replicatable RNA. The molecules are first hybridized to target sequences in a conventional manner.
F R, Kramer, P M, Lizardi
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Biotinylated Probes in Colony Hybridization
1998Colony hybridization is a procedure that allows the detection of cells containing nucleic acid sequences of interest (1). In this method, microbial colonies grown on, or transferred to, a supporting membrane are lysed and their nucleic adds denatured to single strands and fixed in place on the membrane.
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In situ hybridization: Quantitation using radiolabeled hybridization probes
1989Publisher Summary This chapter describes several approaches for the determination of relative cellular hybridization densities using in situ hybridization. In other circumstances that might wish to determine the absolute cellular content of messenger RNA (mRNA), the accurate determination of this second quantity may be more difficult without the ...
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Hybrid Small-Molecule/Protein Fluorescent Probes
Chemical ReviewsHybrid small-molecule/protein fluorescent probes are powerful tools for visualizing protein localization and function in living cells. These hybrid probes are constructed by diverse site-specific chemical protein labeling approaches through chemical reactions to exogenous peptide/small protein tags, enzymatic post-translational modifications ...
Masafumi, Minoshima +4 more
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Probe Labeling and Fluorescence In Situ Hybridization
Current Protocols in Cytometry, 1997AbstractThis unit describes in detail basic protocols for probe labeling, denaturing of in situ target DNA, in situ hybridization, and post‐hybridization washes. Support protocols for probe labeling cover probe purification and quality control.
J, Wiegant, A K, Raap
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Molecular Hybridization Probes Prepared with 4-Aminooxybutylamine
Bioconjugate Chemistry, 1998A versatile method is described for preparing nonradioactive DNA probes for molecular hybridization. The method is based on the transamination reaction of double-stranded DNA with 4-aminooxybutylamine (ABA). To optimize the procedure for obtaining stable and sensitive hybridization probes, time of modification, pH, and reaction temperature were varied.
V A, Adarichev +3 more
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