Results 341 to 350 of about 1,909,880 (377)

Preparation of Nonradioactive Probes for in Situ Hybridization

Methods, 2001
In situ hybridization (ISH) enables the precise localization of RNA targets and provides an avenue to study the temporal and spatial patterns of expression of specific genes. ISH has evolved from being an esoteric technique to one that is routinely used by researchers in many areas of research. A major driving force has been the development of numerous
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In Situ Hybridization for RNA: Nonradioactive Probe: ss cDNA Probe [PDF]

, 2000
The use of single-stranded cD NA (ss cDNA) is more advantageous in sensitivity than that of double-stranded cD NA (ds cDNA) or synthetic oligonucleotide as a probe for in situ hybridization (ISH) (Sugawara et al. 1990). In this chapter, we present a simple procedure for the production of nonradioactive ss cD NA probe by combining the two-step ...
Yoshio Kanemitsu, Takehiko Koji
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DNA Hybridization Probes for the Identification of Phytobacteria [PDF]

, 1987
DNA hybridization probes are potentially powerful tools to identify pg quantities of bacterial DNA in pure or mixed cultures or directly from infected tissues. Colony hybridization techniques allow in situ application of the probes to identify colonies without prior DNA isolation and purification.
D. A. Roth, J. Johnson
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In situ hybridization: Quantitation using radiolabeled hybridization probes

1989
Publisher Summary This chapter describes several approaches for the determination of relative cellular hybridization densities using in situ hybridization. In other circumstances that might wish to determine the absolute cellular content of messenger RNA (mRNA), the accurate determination of this second quantity may be more difficult without the ...
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Preparation of Probes for Hybridization

2000
Preparation of probes for hybridization involves in vitro incorporation of reporter molecules into nuclei acids. These reporters can be incorporated at one or both ends of nucleic acid molecules, giving specific, low density labeled probes. High density labeling is usually achieved by incorporating the reporters uniformly throughout entire length of ...
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Chapter 37 Fluorescence in Situ Hybridization with DNA Probes

1990
Publisher Summary Fluorescence in situ DNA hybridization (FISH) is used to label fluorescently specific nucleic acid sequences in cells or chromosomes. The FISH procedure is used to reveal the location of these sequences and to quantify their copy number.
Barbara J. Trask, Daniel Pinkel
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Express hybridization of molecular colonies with fluorescent probes

Russian Journal of Bioorganic Chemistry, 2007
DNA colonies formed during PCR in a polyacrylamide gel and RNA colonies grown in an agarose gel containing Qbeta replicase can be identified using the procedure of transfer of molecular colonies onto a nylon membrane followed by membrane hybridization with fluorescent oligonucleotide probes.
Chetverina Ev, A. V. Kravchenko, Alexander B. Chetverin, Marina Falaleeva   +3 more
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A DNA--silver nanocluster probe that fluoresces upon hybridization.

Nano letters (Print), 2010
DNA-templated silver nanoclusters (DNA/Ag NCs) are an emerging set of fluorophores that are smaller than semiconductor quantum dots and can have better photostability and brightness than commonly used organic dyes.
H. Yeh, J. Sharma, Jason J. Han, Jennifer S. Martinez, J. Werner   +4 more
semanticscholar   +1 more source

Digoxigenin as an Alternative Probe Labeling for In Situ Hybridization

Diagnostic Molecular Pathology, 1992
In situ hybridization (ISH) techniques have proven to be invaluable tools for diagnostic molecular laboratories in the detection of gene expression and viral infection in cell and tissue samples. Radioactively labeled probes are still widely used for ISH because of their high sensitivity in detection.
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Probe-Based (Hybridization) Methods

2000
The conceptual basis of the probe is outlined on pages 19/20. Probes have a variety of uses, which include: Detection/identification of pathogens Detection of specific (e.g. toxin) genes Localization of an (intracellular) target sequence (in situ hybridization, ISH) Confirmation of correct sequence in the products from nucleic acid
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