Results 351 to 360 of about 1,909,880 (377)

Evolution of Hybridization Probes to DNA Machines and Robots.

Accounts of Chemical Research, 2019
Hybridization probes are RNA or DNA oligonucleotides or their analogs that bind to specific nucleotide sequences in targeted nucleic acids (analytes) via Watson-Crick base pairs to form probe-analyte hybrids.
D. Kolpashchikov
semanticscholar   +1 more source

Detection of RNA Hybridization by Pyrene‐Labeled Probes

ChemBioChem, 2009
AbstractPowerful pyrene probes: Two kinds of pyrene‐labeled oligonucleotides (HNA‐ and RNA‐skeleton probes) were explored. The enhanced fluorescence intensity in the monomer region and the disappearance of aggregate/excimer emission in duplexes has been successfully used to detect the hybridization of oligonucleotides.magnified imageBy covalently ...
Steven De Feyter, Sergey N. Mikhailov, Mark Van der Auweraer, Guojie Wang, Jef Rozenski, Arthur Van Aerschot, Piet Herdewijn, Guy Schepers, Georgii V. Bobkov   +8 more
openaire   +3 more sources

Hybridization-sensitive on-off DNA probe: application of the exciton coupling effect to effective fluorescence quenching.

Chemistry - An Asian Journal, 2008
The design of dyes that emit fluorescence only when they recognize the target molecule, that is, chemistry for the effective quenching of free dyes, must play a significant role in the development of the next generation of functional fluorescent dyes. On
S. Ikeda, A. Okamoto
semanticscholar   +1 more source

Sequence dependence of fluorescence emission and quenching of doubly thiazole orange labeled DNA: effective design of a hybridization-sensitive probe.

Bioconjugate chemistry, 2008
We have designed a doubly thiazole orange labeled nucleoside showing high fluorescence intensity for a hybrid with the target DNA and effective quenching for a single-stranded state.
S. Ikeda, Takeshi Kubota, K. Kino, A. Okamoto   +3 more
semanticscholar   +1 more source

In Situ Hybridization for RNA: Nonradioactive Probe: RNA Probe

2000
Non-radioactive in situ hybridization method is now widely used to detect nucleic acid in tissue sections. However, the results for the detection of tissue mRNA are not always consistent. We have to consider several factors for this purpose. We describe our current methods for the most consistent detection.
openaire   +2 more sources

Rapid identification of hybridization probes for chromosomal walking

Gene, 1987
The presence of repeated elements in restriction fragments used as hybridization probes for chromosomal walking poses a major obstacle to the success of this gene-cloning strategy. This report describes a simple and rapid means of identifying restriction fragments devoid of repeated sequences and therefore useful as hybridization probes for chromosomal
Martin F. Kagnoff, Jennifer Resner, Mark D. Howell, Raleigh K. Austin   +3 more
openaire   +3 more sources

In Situ Hybridization for DNA: Fluorescent Probe

2000
Fluorescence in situ hybridization (FISH) with chromosomespecific probes is one of the non-isotopic in situ hybridization methods that opens up several new areas of cytogenetic investigation by allowing visual determination of the presence and normality of specific genetic sequences in single metaphase or interphase cells (Pinkel 1986a, 1986b).
Mayumi Matsuta, Morimasa Matsuta
openaire   +2 more sources

Identification of human DNA in forensic evidence by loop-mediated isothermal amplification combined with a colorimetric gold nanoparticle hybridization probe

Zeitschrift für Rechtsmedizin, 2014
Khanistha Watthanapanpituck, W. Kiatpathomchai, E. Chu, N. Panvisavas   +3 more
semanticscholar   +1 more source

In Situ Hybridization for RNA: Nonradioactive Probe: ds cDNA Probe

2000
As has been shown previously (Koji et al. 1988; 1990; 1996), nonradioactively labeled probes are widely used for in situ hybridization because they have several advantages compared to radioactive ones.
Hirotaka Matsumi, Tetsu Yano
openaire   +2 more sources

Microsatellite instability typing in serum and tissue of patients with colorectal cancer: comparing real time PCR with hybridization probe and high-performance liquid chromatography

Molecular Biology Reports, 2014
P. Mokarram, M. Rismanchi, M. A. Naeeni, S. Samiee, M. Paryan, Abbas Alipour, Z. Honardar, S. Kavousipour, F. Naghibalhossaini, Zohreh Mostafavi-Pour, Ahmad Monabati, S. V. Hosseni, S. A. Shamsdin   +12 more
semanticscholar   +1 more source