H2 Production from Methyl Viologen–Dependent Hydrogenase Activity Monitored by Gas Chromatography
Bio-Protocol, 2023 Bio-hydrogen production is an eco-friendly alternative to commercial H2 production, taking advantage of natural systems. Microbial hydrogenases play a main role in biological mechanisms, catalyzing proton reduction to molecular hydrogen (H2) formation ...
Nuttavut Kosem
doaj +1 more source
Structure-function analyses of metal-binding sites of HypA reveal residues important for hydrogenase maturation in Helicobacter pylori. [PDF]
PLoS ONE, 2017 The nickel-containing enzymes of Helicobacter pylori, urease and hydrogenase, are essential for efficient colonization in the human stomach. The insertion of nickel into urease and hydrogenase is mediated by the accessory protein HypA. HypA contains an N-
Faith C Blum +6 more
doaj +1 more source
Current Opinion in Biotechnology, 2010 Decades of biophysical study on the hydrogenase (H(2)ase) enzymes have yielded sufficient information to guide the synthesis of analogs of their active sites. Three families of enzymes serve as inspiration for this work: the [FeFe]-H(2)ases, [NiFe]-H(2)ases, and [Fe]-H(2)ases, all of which feature iron centers bound to both CO and thiolate.
Bryan E, Barton +2 more
openaire +2 more sources
Overproduction of the cyanobacterial hydrogenase and selection of a mutant thriving on urea, as a possible step towards the future production of hydrogen coupled with water treatment. [PDF]
PLoS ONE, 2018 Using a combination of various types of genetic manipulations (promoter replacement and gene cloning in replicating plasmid expression vector), we have overproduced the complex hydrogenase enzyme in the model cyanobacterium Synechocystis PCC6803.
Théo Veaudor +5 more
doaj +1 more source
Symbiotic legume nodules employ both rhizobial exo- and endo-hydrogenases to recycle hydrogen produced by nitrogen fixation. [PDF]
PLoS ONE, 2010 BACKGROUND: In symbiotic legume nodules, endosymbiotic rhizobia (bacteroids) fix atmospheric N(2), an ATP-dependent catalytic process yielding stoichiometric ammonium and hydrogen gas (H(2)).
Christopher O Ciccolella +4 more
doaj +1 more source
Delivery of iron-sulfur clusters to the hydrogen-oxidizing [NiFe]-hydrogenases in Escherichia coli requires the A-type carrier proteins ErpA and IscA. [PDF]
PLoS ONE, 2012 During anaerobic growth Escherichia coli synthesizes two membrane-associated hydrogen-oxidizing [NiFe]-hydrogenases, termed hydrogenase 1 and hydrogenase 2.
Constanze Pinske, R Gary Sawers
doaj +1 more source
Structural basis for GTP-dependent dimerization of hydrogenase maturation factor HypB. [PDF]
PLoS ONE, 2012 Maturation of [NiFe]-hydrogenase requires the insertion of iron, cyanide and carbon monoxide, followed by nickel, to the catalytic core of the enzyme. Hydrogenase maturation factor HypB is a metal-binding GTPase that is essential for the nickel delivery ...
Kwok-Ho Chan +3 more
doaj +1 more source
A novel endo-hydrogenase activity recycles hydrogen produced by nitrogen fixation. [PDF]
PLoS ONE, 2009 BACKGROUND: Nitrogen (N(2)) fixation also yields hydrogen (H(2)) at 1:1 stoichiometric amounts. In aerobic diazotrophic (able to grow on N(2) as sole N-source) bacteria, orthodox respiratory hupSL-encoded hydrogenase activity, associated with the cell ...
Gordon Ng +4 more
doaj +1 more source
Homology modeling of the spatial structure of HydSL hydrogenase from purple sulphur bacterium Thiocapsa roseopersicina BBS [PDF]
Компьютерные исследования и моделирование, 2013 The results of homology modeling of HydSL, a NiFe-hydrogenase from purple sulphur bacterium Thiocapsa roseopersicina BBS are presented in this work. It is shown that the models have larger confidence level than earlier published ones; a full-size model ...
Azat Vadimovich Abdullatypov +1 more
doaj +1 more source
Molecular Plant-Microbe Interactions, 2008 The legume host affects the expression of Rhizobium leguminosarum hydrogenase activity in root nodules. High levels of symbiotic hydrogenase activity were detected in R.
Belén Brito +5 more
doaj +1 more source