An Escherichia coli hydrogenase‐3‐type hydrogenase in methanogenic archaea [PDF]
European Journal of Biochemistry, 1998 Methanogenic archaea are known to contain two types of [NiFe] hydrogenases designated F420‐reducing hydrogenase and F420‐non‐reducing hydrogenase. We report here that they additionally contain Escherichia coli hydrogenase‐3‐type [NiFe] hydrogenases.
Künkel, A. +3 more
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How [FeFe]-Hydrogenase Facilitates Bidirectional Proton Transfer
Journal of the American Chemical Society, 2019 Hydrogenases are metalloenzymes that catalyze the conversion of protons and molecular hydrogen, H2. [FeFe]-hydrogenases show particularly high rates of hydrogen turnover and have inspired numerous compounds for biomimetic H2 production.
M. Senger +10 more
semanticscholar +2 more sources
Angewandte Chemie - International Edition, 2015 A novel in situ IR spectroscopic approach is demonstrated for the characterization of hydrogenase during catalytic turnover. E. coli hydrogenase 1 (Hyd-1) is adsorbed on a high surface-area carbon electrode and subjected to the same electrochemical ...
Philip A Ash, Kylie A Vincent
exaly +2 more sources
Key role of hydrogen in regulating hydrogenases and the reductive TCA cycle in a thermophilic, autotrophic sulfur-reducing bacterium [PDF]
Applied and Environmental MicrobiologyMany thermophilic autotrophs in deep-sea hydrothermal vents are anaerobes that require H2 for growth. However, our understanding of how non-methanogenic thermophilic hydrogenotrophs adapt to low H2 conditions is nascent.
Briana C. Kubik, James F. Holden
doaj +2 more sources
Multi-omics analysis reveals the genetic basis for rapid CO2 utilization in the acetogenic bacterium Sporomusa sphaeroides KIAC [PDF]
mSystemsSporomusa sphaeroides KIAC is a novel acetogen isolated from cattle feces that exhibits rapid CO2 utilization. To investigate the molecular basis of this phenotype, we performed a comprehensive multi-omics analysis, including Genome-seq, RNA-seq, dRNA ...
Jiyun Bae +13 more
doaj +2 more sources
A safety cap protects hydrogenase from oxygen attack
Nature Communications, 2021 [FeFe]-hydrogenases are efficient H2-catalysts, yet upon contact with dioxygen their catalytic cofactor (H-cluster) is irreversibly inactivated. Here, we combine X-ray crystallography, rational protein design, direct electrochemistry, and Fourier ...
M. Winkler +13 more
semanticscholar +1 more source
H2 Production from Methyl Viologen–Dependent Hydrogenase Activity Monitored by Gas Chromatography
Bio-Protocol, 2023 Bio-hydrogen production is an eco-friendly alternative to commercial H2 production, taking advantage of natural systems. Microbial hydrogenases play a main role in biological mechanisms, catalyzing proton reduction to molecular hydrogen (H2) formation ...
Nuttavut Kosem
doaj +1 more source
Structure-function analyses of metal-binding sites of HypA reveal residues important for hydrogenase maturation in Helicobacter pylori. [PDF]
PLoS ONE, 2017 The nickel-containing enzymes of Helicobacter pylori, urease and hydrogenase, are essential for efficient colonization in the human stomach. The insertion of nickel into urease and hydrogenase is mediated by the accessory protein HypA. HypA contains an N-
Faith C Blum +6 more
doaj +1 more source
Overproduction of the cyanobacterial hydrogenase and selection of a mutant thriving on urea, as a possible step towards the future production of hydrogen coupled with water treatment. [PDF]
PLoS ONE, 2018 Using a combination of various types of genetic manipulations (promoter replacement and gene cloning in replicating plasmid expression vector), we have overproduced the complex hydrogenase enzyme in the model cyanobacterium Synechocystis PCC6803.
Théo Veaudor +5 more
doaj +1 more source
Current Opinion in Biotechnology, 2010 Decades of biophysical study on the hydrogenase (H(2)ase) enzymes have yielded sufficient information to guide the synthesis of analogs of their active sites. Three families of enzymes serve as inspiration for this work: the [FeFe]-H(2)ases, [NiFe]-H(2)ases, and [Fe]-H(2)ases, all of which feature iron centers bound to both CO and thiolate.
Bryan E, Barton +2 more
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