Results 11 to 20 of about 17,663 (189)
Current Opinion in Biotechnology, 2010 Decades of biophysical study on the hydrogenase (H(2)ase) enzymes have yielded sufficient information to guide the synthesis of analogs of their active sites. Three families of enzymes serve as inspiration for this work: the [FeFe]-H(2)ases, [NiFe]-H(2)ases, and [Fe]-H(2)ases, all of which feature iron centers bound to both CO and thiolate.
Bryan E, Barton +2 more
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Molecular Plant-Microbe Interactions, 2008 The legume host affects the expression of Rhizobium leguminosarum hydrogenase activity in root nodules. High levels of symbiotic hydrogenase activity were detected in R.
Belén Brito +5 more
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BMC Biotechnology, 2007 Background Molecular hydrogen is an environmentally-clean fuel and the reversible (bi-directional) hydrogenase of the cyanobacterium Synechocystis sp.
Wood Thomas K +3 more
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TEMPERATURE-SENSITIVE HYDROGENASE AND HYDROGENASE SYNTHESIS IN A PSYCHROPHILIC BACTERIUM [PDF]
Journal of Bacteriology, 1963 Upadhyay, J. (Washington State University, Pullman)and J. L. Stokes. Temperature-sensitive hydrogenase and hydrogenase synthesis in a psychrophilic bacterium. J. Bacteriol.86:992–998. 1963.—Hydrogenase and its synthesis were more heat-sensitive in psychrophilic strain 82 than in mesophilicEscherichia coli.
J, UPADHYAY, J L, STOKES
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Heterologous expression of Alteromonas macleodii and Thiocapsa roseopersicina [NiFe] hydrogenases in Synechococcus elongatus. [PDF]
PLoS ONE, 2011 Oxygen-tolerant [NiFe] hydrogenases may be used in future photobiological hydrogen production systems once the enzymes can be heterologously expressed in host organisms of interest.
Philip D Weyman +6 more
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Integration of an [FeFe]-hydrogenase into the anaerobic metabolism of Escherichia coli
Biotechnology Reports, 2015 Biohydrogen is a potentially useful product of microbial energy metabolism. One approach to engineering biohydrogen production in bacteria is the production of non-native hydrogenase activity in a host cell, for example Escherichia coli. In some microbes,
Ciarán L. Kelly +6 more
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Heterologous expression and maturation of an NADP-dependent [NiFe]-hydrogenase: a key enzyme in biofuel production. [PDF]
PLoS ONE, 2010 Hydrogen gas is a major biofuel and is metabolized by a wide range of microorganisms. Microbial hydrogen production is catalyzed by hydrogenase, an extremely complex, air-sensitive enzyme that utilizes a binuclear nickel-iron [NiFe] catalytic site ...
Junsong Sun +4 more
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Proceedings of the Japan Academy, Series B, 2013 Hydrogenases are microbial enzymes which catalyze uptake and production of H(2). Hydrogenases are classified into 10 classes based on the electron carrier specificity, or into 3 families, [NiFe]-family (including [NiFeSe]-subfamily), [FeFe]-family and [Fe]-family, based on the metal composition of the active site. H(2) is heterolytically cleaved on the
YAGI, Tatsuhiko, HIGUCHI, Yoshiki
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Turning cellulose waste into electricity: hydrogen conversion by a hydrogenase electrode. [PDF]
PLoS ONE, 2013 Hydrogen-producing thermophilic cellulolytic microorganisms were isolated from cow faeces. Rates of cellulose hydrolysis and hydrogen formation were 0.2 mM L(-1) h(-1) and 1 mM L(-1) h(-1), respectively.
Sergey M Abramov +6 more
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Production of biohydrogen by recombinant expression of [NiFe]-hydrogenase 1 in
Microbial Cell Factories, 2010 Background Hydrogenases catalyze reversible reaction between hydrogen (H2) and proton. Inactivation of hydrogenase by exposure to oxygen is a critical limitation in biohydrogen production since strict anaerobic conditions are required.
Kim Jaoon YH, Jo Byung, Cha Hyung
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