Delivery of iron-sulfur clusters to the hydrogen-oxidizing [NiFe]-hydrogenases in Escherichia coli requires the A-type carrier proteins ErpA and IscA. [PDF]
During anaerobic growth Escherichia coli synthesizes two membrane-associated hydrogen-oxidizing [NiFe]-hydrogenases, termed hydrogenase 1 and hydrogenase 2.
Constanze Pinske, R Gary Sawers
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Structural basis for GTP-dependent dimerization of hydrogenase maturation factor HypB. [PDF]
Maturation of [NiFe]-hydrogenase requires the insertion of iron, cyanide and carbon monoxide, followed by nickel, to the catalytic core of the enzyme. Hydrogenase maturation factor HypB is a metal-binding GTPase that is essential for the nickel delivery ...
Kwok-Ho Chan +3 more
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A widely distributed hydrogenase oxidises atmospheric H2 during bacterial growth
Diverse aerobic bacteria persist by consuming atmospheric hydrogen (H2) using group 1h [NiFe]-hydrogenases. However, other hydrogenase classes are also distributed in aerobes, including the group 2a [NiFe]-hydrogenase.
Zahra F. Islam +6 more
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A novel endo-hydrogenase activity recycles hydrogen produced by nitrogen fixation. [PDF]
BACKGROUND: Nitrogen (N(2)) fixation also yields hydrogen (H(2)) at 1:1 stoichiometric amounts. In aerobic diazotrophic (able to grow on N(2) as sole N-source) bacteria, orthodox respiratory hupSL-encoded hydrogenase activity, associated with the cell ...
Gordon Ng +4 more
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The trace amounts (0.53 ppmv) of atmospheric hydrogen gas (H2) can be utilized by microorganisms to persist during dormancy. This process is catalyzed by certain Actinobacteria, Acidobacteria, and Chloroflexi, and is estimated to convert 75 × 1012 g H2 ...
Rob A. Schmitz +7 more
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Homology modeling of the spatial structure of HydSL hydrogenase from purple sulphur bacterium Thiocapsa roseopersicina BBS [PDF]
The results of homology modeling of HydSL, a NiFe-hydrogenase from purple sulphur bacterium Thiocapsa roseopersicina BBS are presented in this work. It is shown that the models have larger confidence level than earlier published ones; a full-size model ...
Azat Vadimovich Abdullatypov +1 more
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The legume host affects the expression of Rhizobium leguminosarum hydrogenase activity in root nodules. High levels of symbiotic hydrogenase activity were detected in R.
Belén Brito +5 more
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Background Molecular hydrogen is an environmentally-clean fuel and the reversible (bi-directional) hydrogenase of the cyanobacterium Synechocystis sp.
Wood Thomas K +3 more
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Heterologous expression of Alteromonas macleodii and Thiocapsa roseopersicina [NiFe] hydrogenases in Synechococcus elongatus. [PDF]
Oxygen-tolerant [NiFe] hydrogenases may be used in future photobiological hydrogen production systems once the enzymes can be heterologously expressed in host organisms of interest.
Philip D Weyman +6 more
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TEMPERATURE-SENSITIVE HYDROGENASE AND HYDROGENASE SYNTHESIS IN A PSYCHROPHILIC BACTERIUM [PDF]
Upadhyay, J. (Washington State University, Pullman)and J. L. Stokes. Temperature-sensitive hydrogenase and hydrogenase synthesis in a psychrophilic bacterium. J. Bacteriol.86:992–998. 1963.—Hydrogenase and its synthesis were more heat-sensitive in psychrophilic strain 82 than in mesophilicEscherichia coli.
J, UPADHYAY, J L, STOKES
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