BMC Biotechnology, 2007 Background Molecular hydrogen is an environmentally-clean fuel and the reversible (bi-directional) hydrogenase of the cyanobacterium Synechocystis sp.
Wood Thomas K +3 more
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[NiFeSe]-Hydrogenase Chemistry [PDF]
Accounts of Chemical Research, 2015 The development of technology for the inexpensive generation of the renewable energy vector H2 through water splitting is of immediate economic, ecological, and humanitarian interest. Recent interest in hydrogenases has been fueled by their exceptionally high catalytic rates for H2 production at a marginal overpotential, which is presently only matched
Wombwell, Claire +2 more
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Identifying conformational changes with site-directed spin labeling reveals that the GTPase domain of HydF is a molecular switch [PDF]
, 2017 [FeFe]-hydrogenases catalyse the reduction of protons to hydrogen at a complex 2Fe[4Fe4S] center called H-cluster. The assembly of this active site is a multistep process involving three proteins, HydE, HydF and HydG.
Acquasaliente, Laura +8 more
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Heterologous expression of Alteromonas macleodii and Thiocapsa roseopersicina [NiFe] hydrogenases in Synechococcus elongatus. [PDF]
PLoS ONE, 2011 Oxygen-tolerant [NiFe] hydrogenases may be used in future photobiological hydrogen production systems once the enzymes can be heterologously expressed in host organisms of interest.
Philip D Weyman +6 more
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A nonmitochondrial hydrogen production in Naegleria gruberi [PDF]
, 2014 Naegleria gruberi is a free-living heterotrophic aerobic amoeba well known for its ability to transform from an amoeba to a flagellate form. The genome of N.
Anastasios D. Tsaousis +39 more
core +2 more sources
Heterologous expression and maturation of an NADP-dependent [NiFe]-hydrogenase: a key enzyme in biofuel production. [PDF]
PLoS ONE, 2010 Hydrogen gas is a major biofuel and is metabolized by a wide range of microorganisms. Microbial hydrogen production is catalyzed by hydrogenase, an extremely complex, air-sensitive enzyme that utilizes a binuclear nickel-iron [NiFe] catalytic site ...
Junsong Sun +4 more
doaj +1 more source
Metagenomic sequencing unravels gene fragments with phylogenetic signatures of O2-tolerant NiFe membrane-bound hydrogenases in lacustrine sediment [PDF]
, 2015 Many promising hydrogen technologies utilising hydrogenase enzymes have been slowed by the fact that most hydrogenases are extremely sensitive to O2. Within the group 1 membrane-bound NiFe hydrogenase, naturally occurring tolerant enzymes do exist, and ...
Couto, Jillian M. +4 more
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Integration of an [FeFe]-hydrogenase into the anaerobic metabolism of Escherichia coli
Biotechnology Reports, 2015 Biohydrogen is a potentially useful product of microbial energy metabolism. One approach to engineering biohydrogen production in bacteria is the production of non-native hydrogenase activity in a host cell, for example Escherichia coli. In some microbes,
Ciarán L. Kelly +6 more
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High-yield expression of heterologous [FeFe] hydrogenases in Escherichia coli. [PDF]
PLoS ONE, 2010 BACKGROUND: The realization of hydrogenase-based technologies for renewable H(2) production is presently limited by the need for scalable and high-yielding methods to supply active hydrogenases and their required maturases.
Jon M Kuchenreuther +5 more
doaj +1 more source
The Geometry of the Catalytic Active Site in [FeFe]-hydrogenases is Determined by Hydrogen Bonding and Proton Transfer [PDF]
, 2019 [FeFe]-hydrogenases are efficient metalloenzymes that catalyze the oxidation and evolution of molecular hydrogen, H2. They serve as a blueprint for the design of synthetic H2-forming catalysts.
Apfel, Ulf-Peter +11 more
core +2 more sources