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Radioimmunoassay of 17-hydroxyprogesterone
Steroids, 1972Abstract A method is described for the determination of 17-hydroxyprogesterone in peripheral venous plasma (0.1–1.0 ml) from men and women using an antiserum to 17-hydroxyprogesterone-3-carboxymethyl oxime bovine serum albumin (BSA). The coefficients of variation on replicate analyses ranged from 7–16%.
E, Youssefnejadian +3 more
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17α-Hydroxyprogesterone in Human Plasma
The Journal of Clinical Endocrinology & Metabolism, 1971ABSTRACT A shortened approach to the measurement of 17α-hydroxyprogesterone (17-OHP) is described. The extracted plasma sample with 3H indicator is purified by only one paper chromatography. No acetylation step or further chromatography is necessary. Values obtained in males and females are the same as reported by Strott et al.
M, Stewart-Bentley, R, Horton
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A Nonchromatographic Radioimmunoassay for 17α-Hydroxyprogesterone
The Journal of Clinical Endocrinology & Metabolism, 1975A radioimmunoassay is described for the measurement of 17alpha-hydroxyprogesterone in human plasma. We have employed the principle of "immunologic purification." 17-OHP and related steroids are bound to an excess of antiserum. Steroids with a low affinity for the antibody are extracted by ether.
M, Hami, A, Rosler, D, Rabinowitz
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The effect of hydroxyprogesterone on ureteral stones
International Urology and Nephrology, 1988It has been suggested that dilatation of the ureter seen in pregnancy is caused by progesterone. Twenty-four patients with ureteral calculi, therefore, were given an intramuscular injection of 250 mg hydroxyprogesterone in order to facilitate the discharge of the stones. Irrespective of the duration of symptoms there was a potential effect of treatment
A L, Mikkelsen +3 more
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Radioimmunoassay of Plasma 17-Hydroxyprogesterone
The Journal of Clinical Endocrinology & Metabolism, 1971ABSTRACT Plasma 17-hydroxyprogesterone (17-OHP) was measured by radioimmunoassay, using an antibody preparation as specific binding reagent. One ml of plasma, with tritiated 17-OHP added for recovery estimation, was extracted with ether and chromatographed on a microcolumn of Celite.
G E, Abraham +4 more
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Measurement of 17-Hydroxyprogesterone in Human Plasma
The Journal of Clinical Endocrinology & Metabolism, 1968ABSTRACT A sensitive and precise method, based on the technique of competitive proteinbinding, was developed for measuring 17-hydroxyprogesterone (17-OHP) in peripheral blood. The plasma 17-OHP level in normal men was 95 ng/100 ml±31 (sd). In normal women the plasma 17-OHP level was 42 ng/100 ml±20 (sd) during the follicular phase and 174 ng/100 ml ±46
C A, Strott, M B, Lipsett
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The production and characterisation of antisera to 17-hydroxyprogesterone
Journal of Steroid Biochemistry, 1987Sheep were immunised with 11-deoxycortisol-21-hemisuccinate-bovine serum albumin (11-deoxycortisol-21-HS-BSA) or with 17-hydroxyprogesterone-7 alpha-carboxyethyl thioether-keyhole limpet haemocyanin (17-OHP-7 alpha-CETE-KLH) or with 17-OHP-3-(O-carboxymethyl)oxime-KLH (17-OHP-3-CMO-KLH). The resultant antisera were assessed using [3H]17-OHP and dextran-
B A, el-Gamal +4 more
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Biotransformation into 11α-hydroxyprogesterone glucosides by glucosyltransferase
Steroids, 2019Recently, studies on the steroidal hormone activity in the brain have attracted attention, and the influences of the varied glucosides and their artificial derivatives have been discussed; additionally, it has been suggested that glucosides are the synthetic precursors of glucuronide as a label molecule.
Tokutaro Yamaguchi +4 more
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The 17-alpha hydroxyprogesterone chronicle
American Journal of Obstetrics and Gynecology, 2022Jack Fitzsimmons, Wadia Mulla
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Compounded hydroxyprogesterone caproate
American Journal of Obstetrics and Gynecology, 2013John L, Chollet +2 more
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