Results 11 to 20 of about 13,983 (221)

Oligomeric state of hypoxanthine−guanine phosphoribosyltransferase from Mycobacterium tuberculosis [PDF]

Biochimie, 2017
Sedimentation equilibrium and size-exclusion chromatography experiments on Mycobacterium tuberculosis hypoxanthine-guanine phosphoribosyltransferase (MtHGPRT) have established the existence of this enzyme as a reversibly associating mixture of dimeric and tetrameric species in 0.1 M Tris-HCl-0.012 M MgCl2, pH 7.4.
Wai Soon Eng, Dianne T. Keough, Dana Hockova, Donald J. Winzor, Luke W. Guddat   +4 more
openaire   +5 more sources

Investigation of the functional hot-spot residues of an enzyme by real-time monitoring of the enzymatic reaction using NMR and computational approaches [PDF]

Scientific Reports
Favipiravir is an anti-influenza prodrug that is metabolized to its phosphoribosylated form, favipiravir-ribofuranosyl-5′-monophosphate (favipiravir-RMP), by human endogenous enzyme hypoxanthine-guanine phosphoribosyltransferase (HGPRT).
Toshihiko Sugiki, Tomoki Yoshida, Masaki Tsukamoto, Koichiro Miyanishi, Akinori Kagawa, Natsuko Miura, Tomoto Ura, Jun Fukazawa, Yuko Hatanaka, Tsuyoshi Murata, Toshimichi Fujiwara, Masahiro Kitagawa, Yasushi Morita, Kumiko Sakai-Kato, Yoichi Takakusagi, Nobutada Tanaka, Makoto Negoro   +16 more
doaj   +2 more sources

Transcriptomic and Metabolomic Analysis Reveals the Impact of Autophagy Regulation on Purine Content in Mutton [PDF]

Foods
Background: Excessive purine intake increases the risk of hyperuricemia and gout. This study investigates the relationship between purine content in mutton and meat quality traits and explores the regulatory mechanism of purine metabolism through ...
Xu Han, Yang Chen, Dandan Tan, Cuiyu Lai, Xuewen Han, Jinlin Chen, Yu Fu, Xuesong Shan, Huaizhi Jiang   +8 more
doaj   +2 more sources

The Effect of Two Weeks of High-intensity Interval Training on Salvage nucleotide pathway [PDF]

فیزیولوژی ورزش و فعالیت بدنی, 2022
Purpose: The effects of exercise adaptation on improving the purine nucleotides components are inevitable, and physical activity improves the purine nucleotide salvage pathway.
Abbas Ghanbari Niaki, Rouhollah Haghshenas Gatabi   +1 more
doaj   +1 more source

Effects of Immunization with Recombinant Schistosoma mansoni Enzymes AK and HGPRT: Murine Infection Control

Pathogens, 2023
Schistosomiasis is one of the most important human helminthiases worldwide. Praziquantel is the current treatment, and no vaccine is available until the present.
Ana Carolina Maragno Fattori, Elisandra de A. Montija, Bruna D. de L. Fragelli, Ricardo de O. Correia, Cynthia Aparecida de Castro, Larissa Romanello, Camila T. Nogueira, Silmara M. Allegretti, Edson G. Soares, Humberto D. Pereira, Fernanda de F. Anibal   +10 more
doaj   +1 more source

Research Advances in Purine Phosphoribosyltransferases of Protozoan Parasites

Guangdong nongye kexue, 2023
Parasitic protozoa are single-celled organisms that have adapted to live in cells of humans and animals. The protozoan parasites include Leishmania spp., Trypanosoma spp., Plasmodium spp., Toxoplasma gondii, Cryptosporidium spp., and Eimeria spp., which ...
Zhihui YU, Siyun FANG, Mingfei SUN, Nanshan QI, Wenjun LIU, Juan LI, Junjing HU, Shenquan LIAO   +7 more
doaj   +1 more source

Analysis of parameters of purine metabolism in patients with diabetes mellitus type 2

Medičnì Perspektivi, 2023
The aim of this research is to comprehensively analyze the current state of purine metabolism as well as assess its features in patients with diabetes mellitus type 2, taking into account the clinical and metabolic polymorphism of the disease.
A.O. Chernyaeva, M.R. Mykytyuk , Yu.I. Karachentsev   +2 more
doaj   +1 more source

Adeno‐associated virus serotype 2 capsid variants for improved liver‐directed gene therapy

Hepatology, EarlyView., 2022
Abstract Background and Aims Current liver‐directed gene therapies look for adeno‐associated virus (AAV) vectors with improved efficacy. With this background, capsid engineering is explored. Whereas shuffled capsid library screenings have resulted in potent liver targeting variants with one first vector in human clinical trials, modifying natural ...
Nadja Meumann, Marti Cabanes‐Creus, Moritz Ertelt, Renina Gale Navarro, Julie Lucifora, Qinggong Yuan, Karin Nien‐Huber, Ahmed Abdelrahman, Xuan‐Khang Vu, Liang Zhang, Ann‐Christin Franke, Christian Schmithals, Albrecht Piiper, Annabelle Vogt, Maria Gonzalez‐Carmona, Jochen T. Frueh, Evelyn Ullrich, Philip Meuleman, Steven R. Talbot, Margarete Odenthal, Michael Ott, Erhard Seifried, Clara T. Schoeder, Joachim Schwäble, Leszek Lisowski, Hildegard Büning   +25 more
wiley   +1 more source

Context-Specific Metabolic Alterations in HPRT1 Knockout Cells Within a 3D Culture System. [PDF]

Cancer Med
Cancer metabolism studies in 2D culture models often overlook the in vivo complexity. We found that HPRT1 knockout suppressed cell proliferation in 3D and in vivo, but had minimal effect in 2D. Metabolomic analysis also revealed that HPRT1 knockout increased carnosine production via the β‐alanine pathway in 3D cultures. ABSTRACT Background Cancer cells
Tabata S, Fujimoto I, Soga T, Makinoshima H.   +3 more
europepmc   +2 more sources

Lesch-Nyhan Disease and Its Variants: Phenotypic and Mutation Spectrum of Hypoxanthine-Guanine Phosphoribosyltransferase Deficiency in Argentine Patients

Journal of Inborn Errors of Metabolism and Screening, 2021
Hypoxanthine-guanine phosphoribosyltransferase (HPRT) deficiency is a disorder of purine metabolism responsible for Lesch-Nyhan Disease (LND) and its variants, HPRT-related hyperuricemia with neurologic dysfunction (HND) and HPRT-related hyperuricemia ...
Laura E. Laróvere, Lynette D. Fairbanks, H. A. Jinnah, Norberto B. Guelbert, Emilia Escuredo, Adriana Becerra, Raquel Dodelson de Kremer   +6 more
doaj   +1 more source