Results 41 to 50 of about 1,073 (151)

Ribosomal RNA operon copy number: A trait‐informed framework to close the microbial cultivation gap

iMetaOmics, Volume 3, Issue 1, March 2026.
The vast majority of microbes remain uncultured, constraining functional validation and limiting microbiome‐based applications. We show that ribosomal RNA operon (rrn) copy number—a stable, phylogenetically conserved genomic trait—is strongly correlated with cultivation outcomes across ecosystems.
Yuxiao Huang, Qingliu Wu, Chang Liu, Pugang Yu, Hao Li, Shengyue Tang, Fusuo Zhang, Shuangjiang Liu, Bin Ni   +8 more
wiley   +1 more source

Advanced Microfluidics for Single Cell‐Based Cancer Research

Advanced Science, Volume 12, Issue 41, November 6, 2025.
Cutting‐edge microfluidic platforms are transforming single‐cell cancer research. This review highlights advanced technologies, from droplet microfluidics to tumour‐chips, that enable functional and spatial single‐cell analyses. By integrating biosensing, immune components, and patient‐derived materials, these systems offer new insights into tumour ...
Adriana Carneiro, Marta Aranda Palomer, Margarida Esteves, Carolina Rodrigues, José Maria Fernandes, Francisca Oliveira, Alexandra Teixeira, Carlos Honrado, Lorena Dieguez, Sara Abalde‐Cela, Miguel Xavier   +10 more
wiley   +1 more source

Microbial Population Changes in Decaying Ascophyllum nodosum Result in Macroalgal-Polysaccharide-Degrading Bacteria with Potential Applicability in Enzyme-Assisted Extraction Technologies

Marine Drugs, 2019
Seaweeds are of significant interest in the food, pharmaceutical, and agricultural industries as they contain several commercially relevant bioactive compounds.
Maureen W. Ihua, Freddy Guihéneuf, Halimah Mohammed, Lekha M. Margassery, Stephen A. Jackson, Dagmar B. Stengel, David J. Clarke, Alan D. W. Dobson   +7 more
doaj   +1 more source

A promising frontier of circulating messenger RNA in liquid biopsy: From mechanisms to clinical applications

International Journal of Cancer, Volume 157, Issue 8, Page 1519-1537, 15 October 2025.
Abstract Liquid biopsy can revolutionize cancer patient management as repeated sampling allows real‐time monitoring of disease progression and response to treatment. The circulating transcriptome represents a rich source of potential cancer biomarkers, including coding and non‐coding RNAs.
Mengqian Li, Yijin Yu, Guangyue Liu, Shunhong Zhang, Chenchen Luo, Saidi Hu, Siran Wan, Linyong Zhao   +7 more
wiley   +1 more source

Scheme of iChIP analysis of the Pax5 1A promoter.

, 2015
(A) Scheme of iChIP analysis. 8 x LexA-binding elements (LexA BE) were inserted into 0.3 kbp upstream of the transcription start site (TSS) of the Pax5 exon 1A. (B) The insertion site of LexA BE.
Toshitsugu Fujita (25023), Hodaka Fujii (200793), Fusako Kitaura (685165)   +2 more
core   +1 more source

Heterogeneity Landscape of Circulating Tumor Cells in Clinical Utility

Advanced Therapeutics, Volume 8, Issue 10, October 2025.
This study reports the heterogeneity spectrum of circulating tumor cells. The factors involved in the spectrum promote CTCs to overcome barriers in the metastatic cascade but impact the clinical outcome of cancer patients. In summary, the heterogeneities in association with CTCs ultimately reduce patient survival and facilitate increasing mortality of ...
Md Kowsar Alam, Lu Yanjun, Bee Luan Khoo, Tongxu Si, Heng Zou, Mengsu Yang   +5 more
wiley   +1 more source

iChIP analysis of the Pax5 1A promoter.

, 2015
(A) Immunoblot analysis of 3xFNLDD and Pax5 proteins in KI(B) cell lines retaining LexA BE in the Pax5 1A promoter. (B) Expression of Pax5 and AID mRNA in KI(B) #4 (hereafter referred simply as KI(B)).
Toshitsugu Fujita (25023), Hodaka Fujii (200793), Fusako Kitaura (685165)   +2 more
core   +1 more source

Identification of Proteins Interacting with Genomic Regions of Interest in vivo Using Engineered DNA-binding Molecule-mediated Chromatin Immunoprecipitation (enChIP)

Bio-Protocol, 2014
Elucidation of molecular mechanisms of genome functions requires identification of molecules interacting with genomic regions of interest in vivo. To this end, it is useful to isolate the target regions retaining molecular interactions.
Toshitsugu Fujita, Hodaka Fujii
doaj   +1 more source

Circulating Tumor Cell‐Derived Organoids: Current Progress, Applications, and Future

MedComm – Future Medicine, Volume 4, Issue 3, September 2025.
CTC‐derived organoids, which combine the characteristics of patient‐derived organoids and circulating tumor cells (CTCs), represent a novel tool in cancer research. This article reviews the progress, applications, and future prospects of CTC‐derived organoids.
Tiantian Li, Biao Deng, Sheng Li, Yanxia Wu, Zhenghao Lu, Zhu Liang   +5 more
wiley   +1 more source

Microfluidic Systems in Cancer Diagnosis: Advancing Exosome and CTC Detection From Laboratory Research to Clinical Application

Nano Select, Volume 6, Issue 7, July 2025.
Detection of cancer through the identification of EV markers and CTCs using microfluidic systems is rapidly advancing. These markers are typically identified from liquid biopsy samples, which offer a highly favorable alternative to traditional biopsy methods due to their non‐invasive nature.
Bahareh Valinezhad Saghezi, Kamran Mansouri, Rahman Hallaj   +2 more
wiley   +1 more source