Results 141 to 150 of about 97,768,729 (189)
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Identification of iduronate-2-sulfatase in mouse pancreatic islets
American Journal of Physiology-Endocrinology and Metabolism, 2004The lysosomal enzyme iduronate-2-sulfatase (IDS) is expressed in pancreatic islets and is responsible for degradation of proteoglycans, such as perlecan and dermatan sulfate. To determine the role of IDS in islets, expression and regulation of the gene and localization of the enzyme were investigated in mouse pancreatic islets and clonal cells. The Ids
I, Coronado-Pons +4 more
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“Supercharged Cells” for Delivery of Recombinant Human Iduronate-2-Sulfatase
Molecular Genetics and Metabolism, 2000Expression of iduronate-2-sulfatase (IDS) from three different promoters in four retroviral vectors was studied in peripheral blood lymphocytes from patients with Hunter syndrome (PBL(MPS)), i.e., the LTR in vectors L2SN and L2, avian beta-actin promoter in LB2, and the CMV early promoter in LNC2.
D, Pan +4 more
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Brain-Penetrating IgG-Iduronate 2-Sulfatase Fusion Protein for the Mouse
Drug Metabolism and Disposition, 2012Mucopolysaccharidosis (MPS) type II (Hunter's syndrome) is caused by mutations in the iduronate 2-sulfatase (IDS) fusion protein. MPS-II affects the brain, and enzyme replacement therapy is not effective in the brain, because the enzyme does not cross the blood-brain barrier.
Qing-Hui, Zhou +4 more
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Genetic Engineering of a Bifunctional IgG Fusion Protein with Iduronate-2-Sulfatase
Bioconjugate Chemistry, 2009Iduronate-2-sulfatase (IDS) is a lysosomal sulfatase that prevents the accumulation within the brain of glycosoaminoglycans. However, IDS does not cross the blood-brain barrier (BBB). To enable BBB transport, human IDS, minus its signal peptide, was fused to the carboxyl terminus of the heavy chain of a chimeric monoclonal antibody (mAb) to the human ...
Jeff Zhiqiang, Lu +3 more
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Mutations in the iduronate-2-sulfatase gene in five Norwegians with Hunter syndrome
Human Genetics, 1996We have identified the mutations in the iduronate-2-sulfatase (IDS) gene of five unrelated Norwegians with Hunter syndrome by reverse transcription-polymerase chain reaction (RT-PCR) analysis of IDS mRNA followed by single strand conformation polymorphism (SSCP) analysis and cDNA sequencing. One patient had a 5-bp deletion, located at the intron 5/exon
T C, Olsen +6 more
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Intrathecal delivery of recombinant human iduronate-2-sulfatase (idursulfase-IT)
Molecular Genetics and Metabolism, 2011Background: Idursulfase has limited passage through the bloodbrain barrier when administered intravenously. Objectives: We have undertaken a program to develop an intrathecal (IT) formulation of idursulfase to target CNS manifestations of MPS II. Methods: In a safety study, single bolus injections of idursulfase-IT 3 mg (n=12), 30 mg (n=6), or 100 mg ...
P. Calias +11 more
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evaluation of a human recombinant iduronate-2-sulfatase produces in the yeast
Molecular Genetics and Metabolism, 2023María C. Duarte +2 more
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