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Influence of IgE Fragments on IgE Determination
International Archives of Allergy and Immunology, 1990IgE fragments were detected by polyclonal and monoclonal anti-IgE antibodies using Western blot techniques and immunoassays. Other antibodies detected only intact IgE molecules. In the presence of IgE fragments, higher IgE levels were estimated, depending on the epitope specificity of the antibodies used in the IgE determination assays.
C C, Vassella +2 more
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IgE-immunotoxins. II. IgE-ricin A-chain
Immunopharmacology, 1987In order to develop a reagent capable of killing cells with high-affinity IgE Fc receptors, such as mast cells and basophils, ricin A-chain (the toxic portion of ricin) was conjugated to rat IgE myeloma protein, IR 162, via derivatization of the IgE by n-succinimidyl-3-(2-pyridyldithio)propionate (SPDP) thus creating an IgE-immunotoxin. Monensin (10(-7)
H, Boltansky +4 more
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Human IgE+ and IgE− are not equivalent to mouse highly cytokinergic IgE
Journal of Allergy and Clinical Immunology, 2008We have previously defined IgE+ as the IgE on basophils from a subset of highly allergic asthmatic subjects that release histamine after stimulation with histamine-releasing factor (HRF). The mechanism of IgE+ remains an enigma. Recently, there have been reports of monomeric highly cytokinergic IgEs causing mediator release, cytokine release, and ...
Liping, Xie +5 more
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IgE totales et IgE spécifiques
Bio Tribune Magazine, 2003Bien que le dosage des IgE totales et specifiques ne presente pas de difficulte technique particuliere, la decision de creer une paillasse allergie n’est pas chose aisee, meme pour un laboratoire dont le volume d’activite pourrait le justifier. C’est un peu dans cette perspective que l’expose suivant est presente.
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International Archives of Allergy and Immunology, 1987
The gene for a human epsilon chain Fc fragment has been cloned and expressed at a high level in <i>Escherichia coli</i>, and its biological activity in binding to the high-affinity receptors on mast cells and basophils and mediating histamine release has been examined in a variety of assays, including the inhibition of passive cutaneous ...
H J, Gould +3 more
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The gene for a human epsilon chain Fc fragment has been cloned and expressed at a high level in <i>Escherichia coli</i>, and its biological activity in binding to the high-affinity receptors on mast cells and basophils and mediating histamine release has been examined in a variety of assays, including the inhibition of passive cutaneous ...
H J, Gould +3 more
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Current Pediatric Reviews
Abstract: The Hyper IgE Syndromes are rare primary immunodeficiencies characterized by eczema, recurrent skin and respiratory infections and elevated serum IgE levels. Nowadays a genetic-molecular characterization is possible and allows the distinction in various monogenic pathologies, which share some clinical characteristics but also important ...
Serena Gracci +4 more
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Abstract: The Hyper IgE Syndromes are rare primary immunodeficiencies characterized by eczema, recurrent skin and respiratory infections and elevated serum IgE levels. Nowadays a genetic-molecular characterization is possible and allows the distinction in various monogenic pathologies, which share some clinical characteristics but also important ...
Serena Gracci +4 more
openaire +3 more sources
Allergy. European Journal of Allergy and Clinical Immunology, 2018
Ragıp Ertaş +4 more
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Ragıp Ertaş +4 more
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