Results 11 to 20 of about 18,582 (196)

A Modular Cloning Toolkit for the production of recombinant proteins in Leishmania tarentolae

open access: yesMicrobial Cell, 2022
Modular Cloning (MoClo) is based on libraries of standardized genetic parts that can be directionally assembled via Golden Gate cloning in one-pot reactions into transcription units and multigene constructs.
Katrin Hieronimus   +20 more
doaj   +1 more source

Toehold switch based biosensors for sensing the highly trafficked rosewood Dalbergia maritima

open access: yesSynthetic and Systems Biotechnology, 2022
Nucleic acid sensing is a 3 decades old but still challenging area of application for different biological sub-domains, from pathogen detection to single cell transcriptomics analysis.
Paul Soudier   +15 more
doaj   +1 more source

Engineering of High-Yield Recombinant Adeno-Associated Virus Producer Plasmids. [PDF]

open access: yesBiotechnol J
Graphical Abstract and Lay Summary Recombinant adeno‐associated viruses (AAV) are important in gene therapy but their production may be impaired by non‐optimized gene design. In this study, the authors demonstrate that production was best when the AAV replicase gene was in a wild‐type configuration while the complexity of other genetic elements could ...
Radukic MT   +10 more
europepmc   +2 more sources

X-ray absorption spectroscopy study of the chemistry of «invisible» Au in arsenian pyrites [PDF]

open access: yesE3S Web of Conferences, 2019
Arsenian pyrite is an abundant mineral occurring in many geological settings at the Earth’s surface, including hydrothermal ore deposits which are the main source of Au.
Filimonova Olga   +8 more
doaj   +1 more source

A Forward-Design Approach to Increase the Production of Poly-3-Hydroxybutyrate in Genetically Engineered Escherichia coli [PDF]

open access: yes, 2015
Biopolymers, such as poly-3-hydroxybutyrate (P(3HB)) are produced as a carbon store in an array of organisms and exhibit characteristics which are similar to oil-derived plastics, yet have the added advantages of biodegradability and biocompatibility ...
Bower, I   +12 more
core   +3 more sources

Why Plasmodium vivax and Plasmodium falciparum are so different? A tale of two clades and their species diversities

open access: yesMalaria Journal, 2022
The global malaria burden sometimes obscures that the genus Plasmodium comprises diverse clades with lineages that independently gave origin to the extant human parasites.
Ananias A. Escalante   +2 more
doaj   +1 more source

Auxotrophy to Xeno-DNA: an exploration of combinatorial mechanisms for a high-fidelity biosafety system for synthetic biology applications

open access: yesJournal of Biological Engineering, 2018
Background Biosafety is a key aspect in the international Genetically Engineered Machine (iGEM) competition, which offers student teams an amazing opportunity to pursue their own research projects in the field of Synthetic Biology.
Christopher M. Whitford   +9 more
doaj   +1 more source

Implementing adaptive risk management for synthetic biology: Lessons from iGEM's safety and security programme

open access: yesEngineering Biology, 2021
Synthetic biology offers exciting possibilities to deal with local and global challenges over the coming decades. As we make greater use of biological engineering, it will be increasingly important to balance potential risks and benefits. The rate, scale,
Piers Millett, Tessa Alexanian
doaj   +1 more source

‘Can Simple Biological Systems be Built from Standardized Interchangeable Parts?’:Negotiating Biology and Engineering in a Synthetic Biology Competition [PDF]

open access: yes, 2013
Synthetic biology represents a recent attempt to bring engineering principles and practices to working with biology. In practice, the nature of the relationship between engineering and biology in synthetic biology is a subject of ongoing debate.
Calvert, Jane, Frow, Emma
core   +1 more source

Bi-Directional Learning: Identifying Contaminants on the Yurok Indian Reservation. [PDF]

open access: yes, 2019
The Yurok Tribe partnered with the University of California Davis (UC Davis) Superfund Research Program to identify and address contaminants in the Klamath watershed that may be impairing human and ecosystem health.
Cosby, Christine   +7 more
core   +1 more source

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