Results 241 to 250 of about 420,786 (307)

Closing the gap: Oxford Nanopore Technologies R10 sequencing allows comparable results to Illumina sequencing for SNP-based outbreak investigation of bacterial pathogens. [PDF]

J Clin Microbiol
Bogaerts B, Van den Bossche A, Verhaegen B, Delbrassinne L, Mattheus W, Nouws S, Godfroid M, Hoffman S, Roosens NHC, De Keersmaecker SCJ, Vanneste K.   +10 more
europepmc   +1 more source

Decoding Human Placental Cellular and Molecular Responses to Obesity and Fetal Growth

Advanced Science, EarlyView.
Women with obesity often deliver large‐for‐gestational‐age (LGA) infants. Single‐nucleus RNA sequencing of term placenta reveals that hypoxia and TNF‐α signaling in syncytiotrophoblasts are featured in maternal obesity, but inflammatory signatures in Hofbauer cells and response to lipid or carbohydrate metabolism in fibroblasts are specific to LGA.
Hong Jiang, Emilie Derisoud, Denise Parreira, Nayere Taebnia, Paulo R. Jannig, Reza Zandi Shafagh, Allan Zhao, Congru Li, Macarena Ortiz, Manuel Alejandro Maliqueo, Elisabet Stener‐Victorin, Volker M. Lauschke, Qiaolin Deng   +12 more
wiley   +1 more source

Comprehensive Profiling of N6‐methyladnosine (m6A) Readouts Reveals Novel m6A Readers That Regulate Human Embryonic Stem Cell Differentiation

Advanced Science, EarlyView.
This research deciphers the m6A transcriptome by profiling its sites and functional readout effects: from mRNA stability, translation to alternative splicing, across five different cell types. Machine learning model identifies novel m6A‐binding proteins DDX6 and FXR2 and novel m6A reader proteins FUBP3 and L1TD1.
Zhou Huang, Rucong Liu, Zibaguli Wubulikasimu, Wanqing Zhao, Jiaqi Huang, Jiaxuan Wang, Tianyuan Zhang, Rui Fan, Wei Kong, Qinghua Cui, Yang Li, Yuan Zhou   +11 more
wiley   +1 more source

Whole genome sequencing of hepatitis B virus using tiled amplicon (HEPTILE) and probe based enrichment on Illumina and Nanopore platforms [PDF]

Sheila Lumley, Chris Kent, Daisy Jennings, Haiting Chai, George Airey, Elizabeth Waddilove, Marion Delphin, Amy Trebes, Anna McNaughton, Khadija Said Mohammed, Samuel Wilkinson, Yanxia Wu, George MacIntyre-Cockett, Beatrice Kimono, Kwizera Moses Mbonye, Kevin Ojambo, Tongai Maponga, Cedric Tan, Catherine de Lara, Jacqueline Martin, James Campbell, Marije Van Schalkwyk, Dominique Goedhals, Robert Newton, Eleanor Barnes, Nicholas J. Loman, Paolo Piazza, Joshua Quick, M. Azim Ansari, Philippa C. Matthews   +29 more
openalex   +1 more source

Alkyltriphenylphosphonium Binding to Cardiolipin Triggers Oncosis in Cancer Cells

Advanced Science, EarlyView.
Alkyltriphenylphosphonium, exemplified by TPP+‐C14, preferentially accumulates in mitochondria and selectively binds to cardiolipin, a key phospholipid of the inner mitochondrial membrane, causing loss of mitochondrial membrane potential, severe cellular ATP depletion, and calcium imbalance.
Jin Li, Hang Zheng, Yuxing Lin, Ziwei Zhao, Yijia Mai, Zhangrong Lou, Qiang Liu, Zhao Ma, Chengjun Wu   +8 more
wiley   +1 more source

Additional file 1: of A comparison of Illumina and Ion Torrent sequencing platforms in the context of differential gene expression

, 2017
Nicholas F. Lahens, Emanuela Ricciotti, Olga Smirnova, Erik Toorens, Eun Ji Kim, Giacomo Baruzzo, Katharina E. Hayer, Tapan Ganguly, Jonathan Schug, Gregory Grant   +9 more
openalex   +1 more source

Recalibration of mapping quality scores in Illumina short-read alignments improves SNP detection results in low-coverage sequencing data

, 2020
Eliot Cline, Nuttachat Wisittipanit, Tossapon Boongoen, Ekachai Chukeatirote, D. Struss, Anant Eungwanichayapant   +5 more
openalex   +1 more source

Calcium Shock Enables Efficient and Programmable Particle Delivery for Genome Editing Applications

Advanced Science, EarlyView.
Classical transfection and transduction are inefficient, particularly with confluent cells and organoids, and lack cell type‐specific programmability. This study presents calcium shock (CaSh), a method that dramatically improves particle delivery into single cells, colonies, and organoids.
Nicole Vo, Lorena de Oñate, Maximillian Frank, Xian Hu, Sophie M. Blackburn, Dana V. Foss, Jasmine Villegas, Kunica Asija, Dirk Hockemeyer, Hongxia Fu, Raymond J. Monnat, Ross C. Wilson, Benjamin S. Freedman   +12 more
wiley   +1 more source

Complete reference genomes of two ceftriaxone-resistant <i>Neisseria gonorrhoeae</i> strains identified in routine surveillance in Bangkok, Thailand, using Nanopore Q20+ chemistry, VolTRAX V2b, and Illumina sequencing. [PDF]

Microbiol Resour Announc
Sangprasert P, Golparian D, Paopang P, Girdthep N, Lawung R, Gopinath D, Thammawijaya P, Kittiyaowanarn R, Unemo M.   +8 more
europepmc   +1 more source

p16Ink4a‐Positive Hepatocytes Drive Liver Fibrosis Through Activation of LIFR Family Pathway

Advanced Science, EarlyView.
This study found that, following the long‐term CCl4 treatment, p16high hepatocytes appeared in zone 3, spatially co‐localizing with fibrotic areas. A specific cluster of p16high hepatocytes upregulated CTF1/LIF expression which induced HSC activation and further liver fibrosis, as revealed by single cell transcriptomic analysis.
Koji Nishikawa, Teh‐Wei Wang, Satoshi Kawakami, Shota Tanimoto, Kiyoshi Yamaguchi, Taketomo Kido, Masamichi Kimura, Tsunekazu Hishima, Yuki T. Okamura, Satotaka Omori, Takumi Iritani, Toshikaze Chiba, Takehiro Jimbo, Michio Katano, Kansuporn Kamataki, Ryoichi Yokoyama, Eigo Shimizu, Kiminori Kimura, Satoshi Yamzaki, Seiya Imoto, Yoichi Furukawa, Atsushi Miyajima, Yoshikazu Johmura, Makoto Nakanishi   +23 more
wiley   +1 more source