Results 51 to 60 of about 248,967 (264)

Immersion and invariance observers with time-delayed output measurements

Communications in Nonlinear Science and Numerical Simulation, 2016
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Carlos Murguia, Rob H. B. Fey, Henk Nijmeijer   +2 more
openaire   +3 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Temporary Immersion Culture: A Potential In Vitro Culture Method for the Clonal Propagation of Coconut

Horticulturae
As one of the most important members of the palm family, coconut (Cocos nucifera L.) currently faces a substantial gap between demand and production. Current plantings of this crop are aging, and these traditional varieties are susceptible to several ...
Zhihua Mu, Zhiying Li, Amirhossein Bazrafshan, Sundaravelpandian Kalaipandian, Eveline Yee Yan Kong, Julianne Biddle, Naga Prafulla Chandrika Nulu, Steve Adkins   +7 more
doaj   +1 more source

Nickel ion release of niti archwire in variations of immersion time and toothpaste

Majalah Kedokteran Gigi Indonesia, 2022
Nickel-titanium archwires in orthodontic wires have nickel ion releases. The use toothpaste and saliva immersion time may cause its releases. This study aims to prove the difference and interaction between immersion time and toothpaste variations towards
Syamsiar Cahayati, Lusiana Batubara, Diah Ajeng Purbaningrum, Budi Wibowo   +3 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Implicit-explicit time integration for the immersed wave equation

Computers & Mathematics with Applications
Immersed boundary methods simplify mesh generation by embedding the domain of interest into an extended domain that is easy to mesh, introducing the challenge of dealing with cells that intersect the domain boundary. Combined with explicit time integration schemes, the finite cell method introduces a lower bound for the critical time step size ...
Christian Faßbender, Tim Bürchner, Philipp Kopp, Ernst Rank, Stefan Kollmannsberger   +4 more
openaire   +2 more sources

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Can the Immersion Time of PUVA Bath Therapy be Shortened?

Acta Dermato-Venereologica, 2002
Up to now, there are only a few data available concerning the influence of bathing time on skin phototoxicity. We compared the erythemal responses of normal skin to bath PUVA with 8-methoxypsoralen (8-MOP) after 5, 10 and 20 min immersion time. Currently, 20 min is the routinely performed immersion time in many European countries, including Germany ...
Ralf, Schiener, Stefanie C, Behrens-Williams, Hans, Pillekamp, Peter, Kaskel, Ralf Uwe, Peter, Martina, Kerscher   +5 more
openaire   +3 more sources

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Experimental investigation on corrosion behavior of Fe-C- Mn –Cr P/M steels

Materials Research Express
The corrosion behavior of Fe–C–Mn–Cr powder metallurgy (P/M) steels containing chromium contents of 5 wt%, 10 wt%, and 15 wt% was systematically investigated in acidic environments through the weight loss method.
Atalay Bayable Tiruneh, Teshager Awoke Yeshiwas   +1 more
doaj   +1 more source