Results 91 to 100 of about 2,792,911 (252)

Direct replacement of antibodies with molecularly imprinted polymer (MIP) nanoparticles in ELISA - development of a novel assay for vancomycin [PDF]

open access: yes, 2013
A simple and straightforward technique for coating microplate wells with molecularly imprinted polymer nanoparticles (nanoMIPs) to develop ELISA type assays is presented here for the first time.
Caygill, J. S.   +7 more
core   +1 more source

Industrial applications of immobilized enzymes—A review

open access: yes, 2019
The use of immobilized enzymes is now a routine process for the manufacture of many industrial products in the pharmaceutical, chemical and food industry.
A. Basso, S. Serban
semanticscholar   +1 more source

Characterization and multi-step transketolase-ω-transaminase bioconversions in an immobilized enzyme microreactor (IEMR) with packed tube.

open access: yesJournal of Biotechnology, 2013
The concept of de novo metabolic engineering through novel synthetic pathways offers new directions for multi-step enzymatic synthesis of complex molecules. This has been complemented by recent progress in performing enzymatic reactions using immobilized
Amanatuzzakiah Abdul Halim   +2 more
semanticscholar   +1 more source

α-chymotrypsin immobilized enzyme: physical, activity and stability properties [PDF]

open access: yes, 2015
In this study, α-chymotrypsin enzyme was used as a substrate while micropore Y-zeolite, which is HY, USY and NaY as a support. The purpose of this study was to compare the physical properties of zeolite and immobilization of enzyme with zeolite.
Nazir, Mohd. Hakimi, Ngadi, Norzita
core   +1 more source

Editorial: Special Issue — Enzyme Immobilization [PDF]

open access: yesMolecules, 2014
Immobilization of enzymes and proteins is a requirement for many industrial enzyme applications, as this facilitates enzyme recovery and reuse. Bearing in mind this necessity, the coupling of immobilization to the improvement of other enzyme features has been pursued by many researchers, and nowadays immobilization is recognized as a tool to improve ...
openaire   +4 more sources

Parameters Affecting the Performance of Immobilized Enzyme

open access: yes, 2013
Enzyme immobilization has been investigated to improve lipase properties over the past few decades. Different methods and various carriers have been employed to immobilize enzyme.
Dong‐Hao Zhang   +2 more
semanticscholar   +1 more source

Strep-tag II fusion technology for the modification and immobilization of lipase B from Candida antarctica (CALB)

open access: yesJournal of Genetic Engineering and Biotechnology, 2017
Fusion tags – amino acid sequences that are genetically coded to be expressed as attached moieties to a protein – have the potential to enhance the activity of native enzyme, enable specific purification of the enzyme, and promote simple and efficient ...
Sumreet Singh Johar, Joey N. Talbert
doaj   +1 more source

The Science of Enzyme Immobilization

open access: yes, 2020
Protocols for simple immobilization of unstable enzymes are plenty, but the vast majority of them, unfortunately, have not reached their massive implementation for the preparation of improved heterogeneous biocatalyst. In this context, the science of enzyme immobilization demands new protocols capable of fabricating heterogeneous biocatalysts with ...
Guisán, José Manuel   +4 more
openaire   +3 more sources

A Comprehensive Guide to Enzyme Immobilization: All You Need to Know

open access: yesMolecules
Enzyme immobilization plays a critical role in enhancing the efficiency and sustainability of biocatalysis, addressing key challenges such as limited enzyme stability, short shelf life, and difficulties in recovery and recycling, which are pivotal for ...
M. S. Robescu, T. Bavaro
semanticscholar   +1 more source

Comparative study for enzymatic hydrolysis of sugarcane bagasse using free and nanoparticle immobilized holocellulolytic enzyme cocktail

open access: yesWaste Management Bulletin
This study aimed at a comparative analysis of enzymatic hydrolysis of sugarcane bagasse biomass by using a free holocellulase cocktail of an enzyme (Cellic CTec2) and immobilized enzyme system on IOMNP@SiO2-NH2 support to make the process cost-effective.
Arti Yadav   +5 more
doaj   +1 more source

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