Faster Surface Ligation Reactions Improve Immobilized Enzyme Structure and Activity. [PDF]
Chaparro Sosa AF+4 more
europepmc +1 more source
Electrospinning enables the hierarchical assembly of nanofiber‐based scaffolds for tissue engineering. However, encapsulated proteins often undergo structural changes. This study used mesoporous silica nanoparticles (MSNs) as protective carriers, mitigating protein denaturation and supporting controlled release.
Vera Citro+5 more
wiley +1 more source
Study of the geometry of open channels in a layer-bed-type microfluidic immobilized enzyme reactor. [PDF]
Nagy C, Huszank R, Gaspar A.
europepmc +1 more source
SIMPLE ENTRAPMENT OF ALCALASE IN DIFFERENT SILICA XEROGELS USING THE TWO STEPS SOL-GEL METHOD [PDF]
The present study has focused on the entrapment of Alcalase in different xerogels obtained by using various molar ratios of methyltriethoxysilane, dimethyldietoxisilane and tetraethoxysilane.
Chiriac, A.+2 more
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Affinity chromatography with an immobilized RNA enzyme. [PDF]
Agustı́n Vioque, Sidney Altman
openalex +1 more source
Progress in Surface Plasmon and Other Resonance Biosensors for Biomedical Applications
This is the shortened version: Recent advancements in surface plasmon resonance and other optical resonance biosensors for biomedical applications are presented. Advanced sensing strategies are examined for the detection of diverse analytes, integration of nanomaterials and machine learning, and emerging nonplasmonic modes like guided mode resonance ...
Faten Bashar Kamal Eddin+8 more
wiley +1 more source
UniZyme: A Unified Protein Cleavage Site Predictor Enhanced with Enzyme Active-Site Knowledge [PDF]
Enzyme-catalyzed protein cleavage is essential for many biological functions. Accurate prediction of cleavage sites can facilitate various applications such as drug development, enzyme design, and a deeper understanding of biological mechanisms. However, most existing models are restricted to an individual enzyme, which neglects shared knowledge of ...
arxiv
Abstract A strategy for the immobilization of Johnston's Mono(AMidine) catalyst (MAM) onto polystyrene, silica and hybrid silica‐polystyrene nanoparticles is presented. The catalyst activity was evaluated in the stereoselective aza‐Henry reaction leading to the pivotal β‐amino nitroalkane precursors of the anti‐cancer agents Nutlins (with Nutlin‐3a as ...
Sofia Toldo+8 more
wiley +1 more source
On-line Coupling of Aptamer Affinity Solid-Phase Extraction and Immobilized Enzyme Microreactor Capillary Electrophoresis-Mass Spectrometry for the Sensitive Targeted Bottom-Up Analysis of Protein Biomarkers. [PDF]
Salim H+3 more
europepmc +1 more source
Covalent immobilization of horseradish peroxidase onto magnetic nanoparticles [PDF]
The biocatalyst horseradish peroxidase (HRP) was successfully immobilized onto magnetic nanoparticles by the glutaraldehyde method. The influence of pH medium, ionic strength, the initial protein concentration, temperature and the effect of the ...
Campayo Sumalla, Tania
core