Results 241 to 250 of about 1,001,507 (301)

Immobilized enzyme reactors in proteomics

TrAC - Trends in Analytical Chemistry, 2011
Immobilized enzyme reactorsin ...
Yichu Shan, Zhen Liang
exaly   +1 more source

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The bioengineering of immobilized enzymes

Biochimie, 1973
Summary The development of immobilized enzyme reactors depends especially on the efficient production of immobilized enzymes having a long active life under operational conditions. The effect of immobilization by adsorption or covalent-binding on the retention of enzyme activity is described.
M D, Lilly, S P, O'Neill, P, Dunnill
openaire   +2 more sources

NMR of Immobilized Enzymes

2020
Solid-state NMR has become the method of choice for the assessment of protein structure for insoluble objects lacking long-range order. In this context, it is apparent that solid-state NMR is also perfectly poised toward the characterization of immobilized proteins. For these systems, it is possible to understand at the atomic level which perturbations,
Cerofolini L., Ravera E., Fragai M., Luchinat C.   +3 more
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The Science of Enzyme Immobilization

2020
Protocols for simple immobilization of unstable enzymes are plenty, but the vast majority of them, unfortunately, have not reached their massive implementation for the preparation of improved heterogeneous biocatalyst. In this context, the science of enzyme immobilization demands new protocols capable of fabricating heterogeneous biocatalysts with ...
Jose M, Guisan, Fernando, López-Gallego, Juan M, Bolivar, Javier, Rocha-Martín, Gloria, Fernandez-Lorente   +4 more
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Immobilization of enzymes with polyaziridines

Journal of Biotechnology, 1990
A novel method of enzyme immobilization using a low molecular weight prepolymer of tri-functional aziridines which can immobilize enzymes both by covalent attachment and entrapment within a gel matrix is described. The enzymes are immobilized on a solid support and exhibit an excellent retention of enzymatic activity.
L L, Wood, C S, Cobbs, L, Lantz, L, Peng, G J, Calton   +4 more
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Bacteriolysis by immobilized enzymes

Biotechnology and Bioengineering, 1977
AbstractBacteriolytic enzymes produced by Achromobacter lunatus were immobilized in collagen membrane. Intact bacteria such as Pseudomonas solanacearum, Xanthomonas oryzae, Staphylococcus aureus, and Pseudomonas aeruginosa were lyzed with the bacteriolytic enzyme–collagen membrane. Relative activity of the bacteriolytic enzyme–collagen membrane against
I, Karube, T, Suganuma, S, Suzuki
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Immobilized Enzymes and Their Applications

2019
The main goal of enzyme immobilization is the re-use of enzymes for many reaction cycles and extended periods of time. Many novel strategies were developed during the last 20 years. The most common are thec arrier-bound technologies employing synthetic and natural polymers, but also carrier-free enzyme immobilization using chemical crosslinking ...
Cacicedo, Maximiliano Luis, Manzo, Ricardo Martín, Municoy, Sofia, Bonazza, Horacio, Islan, German Abel, Desimone, Martín Federico, Bellino, Martin Gonzalo, Mammarella, Enrique José, Castro, Guillermo Raul   +8 more
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Enzyme immobilization on heparin

Journal of Biomedical Materials Research, 1978
AbstractWe describe the preparation and some of the properties of heparin‐bound α‐chymotrypsin that were obtained via activation of heparin with water‐soluble carbodiimide. Immobilized enzyme has unchanged kinetic characteristics toward low‐molecular‐weight and macromolecular substrates.
V P, Torchilin, E V, Il'ina, Z A, Streltosova, V N, Smirnov, E I, Chazov   +4 more
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Enzyme immobilization in porous solid supports—penetration of immobilized enzyme

Biotechnology and Bioengineering, 1984
AbstractThe process of enzyme immobilization under the diffusion‐controlled regime (i.e., fast attachment of enzyme compared to its diffusion) is modeled and theoretically solved in this article. Simple and compact solutions for the penetration depth of immobilized enzyme and the bulk enzyme concentration versus time are presented.
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Enzyme immobilization on fibrin

Biotechnology and Bioengineering, 1976
The following conclusions can be drawn concerning the utilization of fibrin to immobilized enzyme systems. Fibrin can be used both as a powder or membrane, to covalently immobilize trypsin with retention of activity. Carbon-14 labeled trypsin can be used to estimate the amount of immobilized enzyme on a proteinaceous support.
J G, Dillon, C W, Wade, W H, Daly
openaire   +2 more sources