Human Deoxycytidine Kinase Is a Valuable Biocatalyst for the Synthesis of Nucleotide Analogues [PDF]
, 2019 Natural ribonucleoside-5’-monophosphates are building blocks for nucleic acids which are used for a number of purposes, including food additives. Their analogues, additionally, are used in pharmaceutical applications.
Hellendahl, Katja F. +4 more
core +1 more source
Advanced Biology, EarlyView.The paper explores the creation and characterization of vesicles through biocatalytic Polymerization‐Induced Self‐Assembly (bioPISA), focusing on achieving size uniformity using centrifugation techniques. It examines the effects of stirring speed on vesicle morphology and analyses the internal polymer‐rich structure using fluorescence correlation ...
Andrea Belluati +7 more
wiley +1 more source
Magnetic catechol-chitosan with bioinspired adhesive surface: preparation and immobilization of ω-transaminase. [PDF]
PLoS ONE, 2012 The magnetic chitosan nanocomposites have been studied intensively and been used practically in various biomedical and biological applications including enzyme immobilization.
Kefeng Ni +5 more
doaj +1 more source
, 2017 The microenvironment around an immobilized enzyme molecule significantly influences the properties of the immobilized enzyme. To explore the combined effects of macromolecular crowding and surface charge on the properties of an immobilized enzyme, epoxy ...
Qiong Yang +5 more
semanticscholar +1 more source
Regioselective chemo-enzymatic syntheses of ferulate conjugates as chromogenic substrates for feruloyl esterases [PDF]
arXiv, 2020 Generally, carbohydrate-active enzymes are studied using chromogenic substrates that provide quick and easy color-based detection of enzyme-mediated hydrolysis. In the case of feruloyl esterases, commercially available chromogenic ferulate derivatives are both costly and limited in terms of their experimental application.
arxiv
Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels
Advanced Biology, EarlyView.Bifunctional microgels carrying a linear DNA template and Ni2+‐activated NTA moieties are used as platform for cell‐free protein synthesis and in situ protein immobilization. By varying the concentration of NTA moieties in the microgels, the amount of GFP‐His immobilized inside the microgel and released to the microgel environment can be regulated ...
Anika Kaufmann +2 more
wiley +1 more source
Immobilized enzyme reactors in HPLC and its application in inhibitor screening: A review
Journal of Pharmaceutical Analysis, 2012 This paper sets out to summarize the literatures based on immobilized enzyme bio-chromatography and its application in inhibitors screening in the last decade.
Si-Meng Fang +3 more
doaj
Immobilization of Trichoderma harzianum α-Amylase on Treated Wool: Optimization and Characterization
Molecules, 2014 α-Amylase from Trichoderma harzianum was covalently immobilized on activated wool by cyanuric chloride. Immobilized α-amylase exhibited 75% of its initial activity after 10 runs.
Saleh A. Mohamed +3 more
doaj +1 more source
Design strategy and process optimization for reactors with continuous transport of an immobilized enzyme [PDF]
, 1993 In order to operate a process which uses immobilized enzymes at constant conversion and constant capacity, the refreshment of the enzyme must be continuous.
Luyben, K.Ch.A.M. +2 more
core +2 more sources
Toward Design Principles for Biomolecular Condensates for Metabolic Pathways
Advanced Biology, EarlyView.Biomolecular condensates are membrane‐less compartments found through‐out nature which can serve as reaction crucibles for biochemical processes. This review explores the design strategies underlying how condensates can be used in biotechnology to enhance multistep enzyme cascades including enhancement by mass action and substrate channeling, and ...
Alain A.M. André +3 more
wiley +1 more source