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Alveolar epithelial depletion of βPAK-interacting exchange factor drives spontaneous pulmonary fibrosis by impairing stemness. [PDF]
Kim HB +12 more
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FAM134B Restricts African Swine Fever Virus Capsid Assembly via Reticulophagy and Its Antiviral Activity is Antagonized by the Viral Virulence-Associated Factor pMGF300-2R. [PDF]
Luo R +9 more
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Structural and functional divergence of cancer hotspot mutations in CREBBP. [PDF]
Zhang W +10 more
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Atg23 prevents aberrant fusion of Atg9 vesicles during delivery to autophagosome formation sites. [PDF]
Kimura T +3 more
europepmc +1 more source
Profiling Inflammatory Responses with Microfluidic Immunoblotting [PDF]
Rapid profiling of signaling pathways has been a long sought after goal in biological sciences and clinical medicine. To understand these signaling pathways, their protein components must be profiled.
Timothy T Cornell, Sascha Goonewardena
exaly +2 more sources
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Immunoblotting and Immunodetection
Current Protocols in Immunology, 1998AbstractImmunoblotting (western blotting) is used to identify specific antigens recognized by polyclonal or monoclonal antibodies. This unit provides protocols for all steps, starting with solubilization of the protein samples, usually by means of SDS and reducing agents.
Sean, Gallagher +3 more
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Cold Spring Harbor Protocols, 2020
Immunoblotting allows detection of a protein antigen immobilized on the protein-retaining membrane support such as nitrocellulose or polyvinylidene fluoride (PVDF). The detection of the protein of interest relies on the binding of an antibody that specifically recognizes the protein of interest exposed on the membrane.
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Immunoblotting allows detection of a protein antigen immobilized on the protein-retaining membrane support such as nitrocellulose or polyvinylidene fluoride (PVDF). The detection of the protein of interest relies on the binding of an antibody that specifically recognizes the protein of interest exposed on the membrane.
openaire +2 more sources
Immunoblotting and dot blotting
A variety of methods has been available for many years for the analytical separation of mixtures of proteins into their component parts by electrophoresis in a gel, usually agarose or polyacrylamide. Popular techniques are: zonal electrophoresis in agarose gel or on cellulose acetate membranes; discontinuous electrophoresis in polyacrylamide gel (PAGE);
D I Stott, D I Stott
exaly +3 more sources
Immunoblotting and Immunodetection
Current Protocols in Immunology, 2016AbstractImmunoblotting (western blotting) is used to identify specific antigens recognized by polyclonal or monoclonal antibodies. This unit provides protocols for all steps, starting with solubilization of the protein samples, usually by means of SDS and reducing agents.
Duojiao, Ni, Peng, Xu, Sean, Gallagher
openaire +5 more sources

