Results 131 to 140 of about 335,110 (302)
Advanced Science, EarlyView.Mechanical stress activates BAP31 in chondrocytes. BAP31 competes with ATG14 for binding to STX17, disrupting the STX17–ATG14 complex required for autophagosome–lysosome fusion. The resultant autophagic flux blockade drives the generation of autophagy‐derived exosomes, which mediate pathological cartilage calcification in OA. Chondrocyte‐targeted BAP31
Zhi‐hua Xu +13 more
wiley +1 more source
Buffer Recipes for quantitative immunoblotting - rinsing and blocking agents v1
, 2017 These are the recipes for blocking and rinsing agents for the quantitative immunoblotting protocol from the Campbell Lab.
Doug Campbell, Natalie Donaher
core +1 more source
Advanced Science, EarlyView.This study reveals that NF‐κB1‐driven TRAIP upregulation in ALD correlates with disease severity. Mechanistically, TRAIP directly binds β‐catenin via its CC domain and promotes its K48‐linked ubiquitination and degradation, which is independent of the GSK3β/β‐TrCP pathway.
Zhan Wu +13 more
wiley +1 more source
PROTEOMICS applied to NUTRIGENOMICS: 2DE and Immunoblotting
, 2018 Theoretical-pratical explanation of how 2DE and immunoblotting technology can be involved in the characterization of bioactive compounds of food ...
Cristina Lamberti
core
Analysis of iNOS protein levels in TNBC patients by immunoblotting.
, 2015 Analysis of iNOS protein levels in TNBC patients by immunoblotting.
Yiming Li (40290) +14 more
core +1 more source
Advanced Science, EarlyView.ABSTRACT The LRRK2 G2019S mutation, a Parkinson's disease–linked variant, has been associated with depression‐like phenotypes, but mechanisms remain unclear. We chart age‐dependent behavioral changes and astrocyte reactivity in G2019S mice and define a pathway connecting LRRK2 to TFEB/GDNF signaling, cellular energetics, and inflammation.
Longping Yao +5 more
wiley +1 more source
Immunoblotting analysis of surface-exposed proteins.
, 2014 (A) Five recombinant SEPs purified from E. coli cell lysate were separated by 12% SDS-PAGE and stained by G-250 Coomassie Brilliant Blue. (B) Immunoblotting analysis of SEPs: lane M, protein molecular mass markers; lanes 1 to 5, rTolC, rPorin_4, rAdr1 ...
Xiaolu Xiong (436303) +5 more
core +1 more source
Citation: 'immunoblot' in the IUPAC Compendium of Chemical Terminology, 5th ed.; International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.13276 • License: The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International for individual terms.
openaire +1 more source
Advanced Science, EarlyView.Our research unveiled a regulatory paradigm wherein TRIM25 orchestrates the ubiquitin‐mediated degradation of UGDH. UGDH modulates the protein stability of TJP1 by regulating O‐GlcNAcylation levels, effectively impeding the metastasis of ccRCC. Our insights elevate UGDH to a pivotal biomarker and tumor suppressor, marking the first demonstration that ...
Xiaolin Chen +13 more
wiley +1 more source