Results 91 to 100 of about 338,330 (210)
Lysis of Cultured Animal Cells, Yeast, and Bacteria for Immunoblotting
, 2010 INTRODUCTIONThis protocol describes cell lysis for immunoblotting. Cell lysis with detergents is commonly used with cultured animal cells. Typically, a 2% solution of the ionic detergent sodium dodecyl sulfate (SDS) is sufficient for lysing cells for the
Hong Ji
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PROTEOMICS applied to NUTRIGENOMICS: 2DE and Immunoblotting
, 2018 Theoretical-pratical explanation of how 2DE and immunoblotting technology can be involved in the characterization of bioactive compounds of food ...
Cristina Lamberti
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Immunoreactivity of canine, feline, and equine D‐dimer with antibodies to human D‐dimer
Journal of Veterinary Internal MedicineBackground Commercially available D‐dimer assays use antibodies against human D‐dimer, with limited sensitivity and specificity data in companion animals.
Juliet E. Brown +2 more
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پزشکی بالینی ابن سینا, 2005 Western blotting or immunoblotting commonly use for study of reaction between antigens and antibodies. Denaturation of many proteins in immunoblotting can affect greatly the reactivity of antibodies and outcome of the procedure.
Ramin Karimi +4 more
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, 2013 AGS Cells were transfected with plasmid coding for VCP-Flag or empty vector, pretreated with MG-132 for 3 hr, and incubated for 6 hr with H. pylori. Immunoprecipitations were performed with anti-Flag M2 antibody affinity gel and the immunoprecipitates ...
Cheng-Chou Yu (278753) +6 more
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Immunoblotting and 77 K fluorescence analysis.
, 2013 A. Immunoblotting analysis of thylakoid membranes isolated from wt and stn7, exposed to 20 min of anaerobic condition. B,C. 77 K fluorescence emission spectra recorded for thylakoid membranes isolated from wt and stn7, exposed to 20 min of anaerobic ...
Sreedhar Nellaepalli (297688) +3 more
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Immunoblotting of cervical cancer tissue extracts.
, 2016 Whole (W), cytoplasmic (C), and nuclear (N) fractions were prepared from tissues of one adenocarcinoma (ADC) and five squamous cell carcinomas (SqCC), and GS28 expression in the fractions was examined by immunoblotting using the anti-GS28 antibody ...
Oh-Joo Kwon (3112221) +5 more
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Experimental PhysiologyWe examined how resistance exercise (RE), cycling exercise and disuse atrophy affect myosin heavy chain (MyHC) protein fragmentation. The 1boutRE study involved younger men (n = 8; 5 ± 2 years of RE experience) performing a lower body RE bout with vastus
Daniel L. Plotkin +16 more
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Effect of protein kinase N1 on the biological behavior of human glioma
Chinese Journal of Contemporary Neurology and Neurosurgery, 2020 Objective To study the expression of protein kinase N1 (PKN1) in glioma and its influence on the biological behavior of glioma cells. Methods The expression of PKN1 gene was verified via GEPIA database, and LN229, U87 and A172 gliomas cells were detected
Yu⁃bing HAO +5 more
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