Results 171 to 180 of about 338,330 (210)

Diagnosis of Paragonimiasis by Immunoblot

The American Journal of Tropical Medicine and Hygiene, 1988
A sensitive and specific immunoblot assay was used to rapidly and accurately diagnose paragonimiasis. The immunoreactivity of a complex Paragonimus westermani Chaffee antigen was evaluated by SDS-PAGE and Western blot analysis. Initial probing with pooled human serum from proven Paragonimus infections revealed many bands, including a significant ...
S B, Slemenda, S E, Maddison, E C, Jong, D D, Moore   +3 more
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Preparing Immunoprecipitations for Immunoblotting

Cold Spring Harbor Protocols, 2020
Immunoprecipitated proteins can be readily analyzed by immunoblotting. Proteins can be efficiently eluted from the Protein A or similar beads by addition of the SDS-PAGE sample loading buffer and heating at 95°C. This elution procedure will also remove the capturing antibody from the beads unless the antibody was cross-linked to the beads ...
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Stripping of the Immunoblot for Reprobing

Cold Spring Harbor Protocols, 2020
For most immunoblots developed with chemiluminescence or with fluorochrome-based detection systems, it is possible to remove the primary and secondary antibodies from the membrane without affecting the bound antigen. This allows you to reuse the membrane for detection of another protein antigen.
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Visualization of the Insulin Receptor by Immunoblotting

Diabetes, 1984
Insulin receptors from rat hepatoma cells (Fao) and human placenta were partially purified by detergent solu-bilization and lectin purification. The insulin receptor preparations were subjected to sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis under reducing or nonreducing conditions.
R, Maron, C R, Kahn, S, Jacobs, Y, Fujita-Yamaguchi   +3 more
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Detection of an Antigen on an Immunoblot

Cold Spring Harbor Protocols, 2019
Detection of the antigen on an immunoblot can be achieved by either enzyme-based detection systems or using detection reagents labeled with fluorochromes. Two major types of enzyme-based detection systems are used in immunoblotting based on either horseradish peroxidase (HRP)–or alkaline phosphatase (AP)–coupled antibodies, and a range of soluble ...
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Protein Blotting and Immunoblotting

2000
Immunoblotting was first devised by Towbin et al. (1979) to exploit the specificity of the reaction between antibodies and proteins transferred from SDS- polyacrylamide electrophoretic gels onto nitrocellulose. Immunoreactive bands were evidenced by labelled “second antibody” or protein A.
BINI, LUCA, Liberatori S., MAGI, BARBARA, MARZOCCHI, BARBARA, Raggiaschi R., Pallini V.   +5 more
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Affinity Immunoblotting

Journal of Immunological Methods, 1986
K A, Knisley, L S, Rodkey
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Determination of Autoantibodies by Line Immunoblotting

2018
Autoantibody detection is a useful and obligatory tool for clinicians and researchers in diagnosing autoimmune diseases. Line blotting is a simple, sensitive, and flexible technique for fast semiquantitative detection of multiple antibodies. Line blotting enables the detection of antibodies on membrane strips coated with thin parallel lines of several ...
Sternbæk, Louise, Friis, Tina, Houen, Gunnar   +2 more
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Immunoblotting 2DE Membranes

2009
Two-dimensional electrophoresis (2DE) immunoblotting combines the resolving power of 2DE and the selectivity of antibodies, allowing the selection of individual or sets of proteins from the total proteome. It is essential when immunoblotting membranes that reproducible 2DE gels are obtained consistently.
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Immunoblotting of Integrins

2003
M, Schneller, W, Arap, R, Pasqualini
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