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Immunocytochemistry on the Thinprep® processor

Diagnostic Cytopathology, 1997
For cytologic specimens, the vast majority of immunocytochemical studies (ICC) are performed on non-gynecologic specimens for diagnostic purposes, and they can be performed on unstained or previously stained direct smears. Although the ThinPrep processor (TPP) has been approved for the preparation of non-gynecologic specimens, there is scant literature
David J. Dabbs   +4 more
openaire   +3 more sources

IMMUNOCYTOCHEMISTRY

1999
Publisher Summary The outcome of immunoelectron microscope methods depends on the type of antibody used and is influenced by the methods of tissue preparation. Antigens vary considerably with respect to their sensitivity to the method of fixation and tissue processing.
Arvid B. Maunsbach, Björn A. Afzelius
openaire   +2 more sources

Immunocytochemistry of Acidophil

Archives of Neurology, 1969
IN A REVIEW of the ultrastructual organization of human acidophil and chromophobe pituitary adenomas, the acidophils were from abundantly to sparsely supplied with secretory granules measuring 300 to 500 mμ, and the secretory granules in chromophobe adenomas were of two sizes, the average diameter being about 100 mμ in some cells and approximately 300 ...
Hajime Handa   +5 more
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On the preparation of cryosections for immunocytochemistry

Journal of Ultrastructure Research, 1984
The key preparation steps in the Tokuyasu thawed frozen section technique for immunocytochemistry, namely freezing, sectioning, thawing, and drying, were studied. A spherical tissue culture cell was used as a model system. The frozen hydrated section technique indicated that glutaraldehyde-fixed, 2.1 M sucrose-infused pellets of cells were routinely ...
Griffiths, Gareth   +3 more
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Specificity controls for immunocytochemistry

Anatomy and Embryology, 2006
Antibodies have been in widespread use for more than three decades as invaluable tools for the specific detection of proteins or other molecules in biological samples. In spite of such a long experience, the field of immunocytochemistry is still troubled by spurious results due to insufficient specificity of antibodies or procedures used.
Silvia Holmseth   +2 more
openaire   +3 more sources

An automated device for immunocytochemistry

Journal of Immunological Methods, 1988
A microprocessor controlled device for automation of immunoenzyme histological and cytological staining procedures is described. As the sequence of processing is controlled by a computer program, the apparatus is flexible and the possibility of technical errors is reduced.
E Stark, R Von der Fecht, D Faltinat
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Application of cryoultramicrotomy to immunocytochemistry

Journal of Microscopy, 1986
SUMMARYThis paper reviews the most recent status of immuno‐cryoultramicrotomy. The technical aspects of each step of the method are also analysed in detail with the intention of providing a useful source of information for investigators using this method.
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Immunocytochemistry of neurotransmitter receptors

Journal of Electron Microscopy Technique, 1990
AbstractOver the last several years our knowledge of neurotransmitter receptors has increased dramatically as receptor types and subtypes have been identified through the development of selective antagonists, neuropharmacological studies, and radioactive ligand binding studies.
Richard A. Altschuler   +2 more
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Immunocytochemistry

2000
Immunocytochemistry provides an accurate way of localizing antigens in situ in tissue sections or cell preparations using antibodies combined with a label that can be identified microscopically. The most frequently used labels are fluorescent compounds that can be excited by light of specific wavelength, enzymes that can act on a specific substrate ...
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Immunocytochemistry in Cytology

1988
In several reviews (Nadji 1980; Walts and Said 1983; Koss 1984; Chess and Hajdu 1986) the considerable value of the application of immunocytochemical methods in cytology (ICC) has been indicated. However, despite the high number of reports focused on the application of ICC as a diagnostic tool it did not have the enormous practical impact of ...
H. Höfler, G. Wirnsberger, H. P. Dinges
openaire   +2 more sources

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