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Immunocytochemistry of the cell nucleus
Electron Microscopy Reviews, 1990This electron microscopic review addresses in situ immunocytochemistry of the mammalian cell nucleus with special reference to the use of autoantibodies, which are the major source of antinuclear antibodies. The localization of many key nuclear antigens is documented and immunocytochemical data are related to the major functional processes of ...
Ivan Raška +2 more
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Chapter 14 Cytology and Immunocytochemistry
1986Publisher Summary This chapter describes techniques that worked out primarily for use with Strongylocentrotus purpuratus and Lytechinus pictus . The chapter discusses whole mounts of fixed and embedded material, sectioned and stained material for bright-field light microscopy, and indirect immunofluorescence microscopy and other fluorochrome ...
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Principles of Immunocytochemistry
1985This outline will: 1. discuss the reasons for avoiding the covalent labeling of second antibodies in immunocytochemistry. 2. present a comparison between PAP and ABC methods. 3. discuss advantages and precautions in production and use of monoclonal antibodies for immunocytochemistry. 4.
L. A. Sternberger, N. H. Sternberger
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Immunocytochemistry in Diagnostic Cytology
1993As the decision for immunocytochemistry is usually made on the basis of findings in Papanicolaou-stained smears and uncovering of the smears takes time, the immunocytochemical results are often reported with some delay. But they are of clinical interest only if reported within a short time.
Guido Sauter +5 more
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Ultrastructural Cytochemistry and Immunocytochemistry
1991A number of histochemical reactions can, with certain modifications, be usefully applied at the ultrastructural level. The value of this approach is that chemical or biochemical information can be related to cell ultrastructure. Although it is not in the general scope of this book to cover all reaction types in ultrastructural cytochemistry a few ...
van Deurs, Bo +2 more
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Basic Immunocytochemistry for Light Microscopy
2003Immunocytochemistry, the identification of cell- or tissue-bound antigens in situ, by means of a specific antibody-antigen reaction, tagged microscopically by a visible label, has a remarkably wide range of applications. The basic techniques are straightforward and can be adapted to explore the localisation of virtually any molecule of interest to the ...
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Immunocytochemistry and sperm pathology.
Journal of submicroscopic cytology and pathology, 1988In this paper the Authors describe the localization of some significative proteins (acrosin, actin, tubulin, vimentin) detected by immunocytochemical methods in human infertile spermatozoa and correlate their distribution with the ultrastructural characteristics of the same spermatozoa, in order to clarify the structural bases of infertility.
Baccetti, B. +5 more
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Immunocytochemistry of NMDA Receptors [PDF]
Ronald S. Petralia, Robert J. Wenthold
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Immunocytochemistry in Histology and Cytology
2018Immunohistochemistry (IHC) helps to visualize the recognition of the antigen in the tissue with the help of corresponding antibody. The present chapter elucidates the principle of IHC and detailed description of the different types of antigen-antibody detection methods.
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